PMID- 27517951
OWN - NLM
STAT- MEDLINE
DCOM- 20170926
LR  - 20181113
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 8
IP  - 8
DP  - 2016 Aug 9
TI  - Experimental Approaches to Study Genome Packaging of Influenza A Viruses.
LID - 10.3390/v8080218 [doi]
LID - 218
AB  - The genome of influenza A viruses (IAV) consists of eight single-stranded 
      negative sense viral RNAs (vRNAs) encapsidated into viral ribonucleoproteins 
      (vRNPs). It is now well established that genome packaging (i.e., the 
      incorporation of a set of eight distinct vRNPs into budding viral particles), 
      follows a specific pathway guided by segment-specific cis-acting packaging 
      signals on each vRNA. However, the precise nature and function of the packaging 
      signals, and the mechanisms underlying the assembly of vRNPs into sub-bundles in 
      the cytoplasm and their selective packaging at the viral budding site, remain 
      largely unknown. Here, we review the diverse and complementary methods currently 
      being used to elucidate these aspects of the viral cycle. They range from 
      conventional and competitive reverse genetics, single molecule imaging of vRNPs 
      by fluorescence in situ hybridization (FISH) and high-resolution electron 
      microscopy and tomography of budding viral particles, to solely in vitro 
      approaches to investigate vRNA-vRNA interactions at the molecular level.
FAU - Isel, Catherine
AU  - Isel C
AD  - Architecture et Reactivite de l'ARN, Universite de Strasbourg, Centre National de 
      la Recherche Scientifique (CNRS), Institut de Biologie Moleculaire et Cellulaire 
      (IBMC), 15 rue Rene Descartes, 67084 Strasbourg, France. 
      c.isel@ibmc-cnrs.unistra.fr.
AD  - Departement de Virologie, Unite de Genetique Moleculaire des Virus a ARN, 
      Institut Pasteur, 75015 Paris, France. c.isel@ibmc-cnrs.unistra.fr.
FAU - Munier, Sandie
AU  - Munier S
AD  - Departement de Virologie, Unite de Genetique Moleculaire des Virus a ARN, 
      Institut Pasteur, 75015 Paris, France. sandie.munier@pasteur.fr.
AD  - Centre National de la Recherche Scientifique (CNRS), Unite Mixte de Recherche 
      3569, 75016 Paris, France. sandie.munier@pasteur.fr.
AD  - Unite de Genetique Moleculaire des Virus a ARN, Sorbonne Paris Cite, Universite 
      Paris Diderot, 75013 Paris, France. sandie.munier@pasteur.fr.
FAU - Naffakh, Nadia
AU  - Naffakh N
AD  - Departement de Virologie, Unite de Genetique Moleculaire des Virus a ARN, 
      Institut Pasteur, 75015 Paris, France. nadia.naffakh@pasteur.fr.
AD  - Centre National de la Recherche Scientifique (CNRS), Unite Mixte de Recherche 
      3569, 75016 Paris, France. nadia.naffakh@pasteur.fr.
AD  - Unite de Genetique Moleculaire des Virus a ARN, Sorbonne Paris Cite, Universite 
      Paris Diderot, 75013 Paris, France. nadia.naffakh@pasteur.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20160809
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
SB  - IM
MH  - Electron Microscope Tomography
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Influenza A virus/*physiology
MH  - Microscopy, Electron, Transmission
MH  - Reverse Genetics
MH  - Single Molecule Imaging
MH  - Virology/*methods
MH  - *Virus Assembly
PMC - PMC4997580
OTO - NOTNLM
OT  - RNA-RNA interaction
OT  - competitive reverse genetics
OT  - influenza virus
OT  - packaging assay
OT  - packaging signal
OT  - single-molecule FISH
EDAT- 2016/08/16 06:00
MHDA- 2017/09/28 06:00
PMCR- 2016/08/01
CRDT- 2016/08/13 06:00
PHST- 2016/06/10 00:00 [received]
PHST- 2016/07/26 00:00 [revised]
PHST- 2016/08/01 00:00 [accepted]
PHST- 2016/08/13 06:00 [entrez]
PHST- 2016/08/16 06:00 [pubmed]
PHST- 2017/09/28 06:00 [medline]
PHST- 2016/08/01 00:00 [pmc-release]
AID - v8080218 [pii]
AID - viruses-08-00218 [pii]
AID - 10.3390/v8080218 [doi]
PST - epublish
SO  - Viruses. 2016 Aug 9;8(8):218. doi: 10.3390/v8080218.