PMID- 27518582
OWN - NLM
STAT- MEDLINE
DCOM- 20170203
LR  - 20171024
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 86
DP  - 2016 Dec 15
TI  - Label-free immunoassay for porcine circovirus type 2 based on excessively tilted 
      fiber grating modified with staphylococcal protein A.
PG  - 1054-1060
LID - S0956-5663(16)30731-X [pii]
LID - 10.1016/j.bios.2016.07.100 [doi]
AB  - Using excessively tilted fiber grating (Ex-TFG) inscribed in standard single mode 
      fiber, we developed a novel label-free immunoassay for specific detection of 
      porcine circovirus type 2 (PCV2), which is a minim animal virus. Staphylococcal 
      protein A (SPA) was used to modify the silanized fiber surface thus forming a SPA 
      layer, which would greatly enhance the proportion of anti-PCV2 monoclonal 
      antibody (MAb) bioactivity, thus improving the effectiveness of specific 
      adsorption and binding events between anti-PCV2 MAbs and PCV2 antigens. 
      Immunoassay experiments were carried out by monitoring the resonance wavelength 
      shift of the proposed sensor under different PCV2 titer levels. Anti-PCV2 MAbs 
      were thoroughly dissociated from the SPA layer by treatment with urea, and 
      recombined to the SPA layer on the sensor surface for repeated immunoassay of 
      PCV2. The specificity of the immunosensor was inspected by detecting porcine 
      reproductive and respiratory syndrome virus (PRRSV) first, and PCV2 subsequently. 
      The results showed a limit of detection (LOD) for the PCV2 immunosensor of 
      ~9.371TCID50/mL, for a saturation value of ~4.801x10(3)TCID50/mL, with good 
      repeatability and excellent specificity.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Luo, Binbin
AU  - Luo B
AD  - Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, 
      Chongqing University of Technology, Chongqing 400054, China; School of 
      Opto-electronic Information, University of Electronic Science and Technology of 
      China, Chengdu 610054, China; Aston Institute of Photonic Technologies, Aston 
      University, Birmingham B4 7ET, UK.
FAU - Wu, Shengxi
AU  - Wu S
AD  - School of Pharmacy and Bioengineering, Chongqing University of Technology, 
      Chongqing 400054, China. Electronic address: sxwu2004@cqut.edu.cn.
FAU - Zou, Wengen
AU  - Zou W
AD  - Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, 
      Chongqing University of Technology, Chongqing 400054, China.
FAU - Zhang, Zhonghao
AU  - Zhang Z
AD  - School of Pharmacy and Bioengineering, Chongqing University of Technology, 
      Chongqing 400054, China.
FAU - Zhao, Mingfu
AU  - Zhao M
AD  - Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, 
      Chongqing University of Technology, Chongqing 400054, China.
FAU - Shi, Shenghui
AU  - Shi S
AD  - Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, 
      Chongqing University of Technology, Chongqing 400054, China.
FAU - Liu, Yong
AU  - Liu Y
AD  - School of Opto-electronic Information, University of Electronic Science and 
      Technology of China, Chengdu 610054, China.
FAU - Xi, Xiangfeng
AU  - Xi X
AD  - National Research Center for Veterinary Medicine, Henan 471003, China.
FAU - Zeng, Zheng
AU  - Zeng Z
AD  - Chongqing Animal Disease Prevention and Control Center, Chongqing 401120, China.
FAU - Liang, Wangwang
AU  - Liang W
AD  - Chongqing Animal Disease Prevention and Control Center, Chongqing 401120, China.
FAU - Yan, Zhijun
AU  - Yan Z
AD  - Aston Institute of Photonic Technologies, Aston University, Birmingham B4 7ET, 
      UK.
FAU - Zhang, Lin
AU  - Zhang L
AD  - Aston Institute of Photonic Technologies, Aston University, Birmingham B4 7ET, 
      UK.
LA  - eng
PT  - Journal Article
DEP - 20160729
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Staphylococcal Protein A)
SB  - IM
MH  - Circovirus/classification/*immunology/*isolation & purification
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Immunoassay/*instrumentation
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Staining and Labeling
MH  - Staphylococcal Protein A/*immunology
OTO - NOTNLM
OT  - Excessively tilted fiber grating (Ex-TFG)
OT  - Label-free immunoassay
OT  - Monoclonal antibody (MAb)
OT  - Porcine circovirus type 2 (PCV2)
OT  - Staphylococcal protein A (SPA)
EDAT- 2016/08/16 06:00
MHDA- 2017/02/06 06:00
CRDT- 2016/08/13 06:00
PHST- 2016/04/29 00:00 [received]
PHST- 2016/07/21 00:00 [revised]
PHST- 2016/07/27 00:00 [accepted]
PHST- 2016/08/13 06:00 [entrez]
PHST- 2016/08/16 06:00 [pubmed]
PHST- 2017/02/06 06:00 [medline]
AID - S0956-5663(16)30731-X [pii]
AID - 10.1016/j.bios.2016.07.100 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2016 Dec 15;86:1054-1060. doi: 10.1016/j.bios.2016.07.100. 
      Epub 2016 Jul 29.