PMID- 27519314
OWN - NLM
STAT- MEDLINE
DCOM- 20170327
LR  - 20231213
IS  - 1471-9053 (Electronic)
IS  - 0032-0781 (Linking)
VI  - 57
IP  - 11
DP  - 2016 Nov
TI  - Combination of Synthetic Chemistry and Live-Cell Imaging Identified a Rapid Cell 
      Division Inhibitor in Tobacco and Arabidopsis thaliana.
PG  - 2255-2268
AB  - Cell proliferation is crucial to the growth of multicellular organisms, and thus 
      the proper control of cell division is important to prevent developmental arrest 
      or overgrowth. Nevertheless, tools for controlling cell proliferation are still 
      poor in plant. To develop novel tools, we focused on a specific compound family, 
      triarylmethanes, whose members show various antiproliferative activities in 
      animals. By combining organic chemistry to create novel and diverse compounds 
      containing the triarylmethyl moiety and biological screens based on live-cell 
      imaging of a fluorescently labeled tobacco Bright Yellow-2 (BY-2) culture cell 
      line (Nicotiana tabacum), we isolated (3-furyl)diphenylmethane as a strong but 
      partially reversible inhibitor of plant cell division. We also found that this 
      agent had efficient antiproliferative activity in developing organs of 
      Arabidopsis thaliana without causing secondary defects in cell morphology, and 
      induced rapid cell division arrest independent of the cell cycle stage. Given 
      that (3-furyl)diphenylmethane did not affect the growth of a human cell line 
      (HeLa) and a budding yeast (Saccharomyces cerevisiae), it should act specifically 
      on plants. Taking our results together, we propose that the combination of 
      desired chemical synthesis and detailed biological analysis is an effective tool 
      to create novel drugs, and that (3-furyl)diphenylmethane is a specific 
      antiproliferative agent for plants.
CI  - (c) The Author 2016. Published by Oxford University Press on behalf of Japanese 
      Society of Plant Physiologists. All rights reserved. For permissions, please 
      email: journals.permissions@oup.com.
FAU - Nambo, Masakazu
AU  - Nambo M
AD  - Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, 
      Chikusa-ku, Nagoya, Aichi, 464-8601 Japan m-ueda@itbm.nagoya-u.ac.jp 
      mnambo@itbm.nagoya-u.ac.jp.
AD  - These authors contributed equally to this work.
FAU - Kurihara, Daisuke
AU  - Kurihara D
AD  - Division of Biological Science, Graduate School of Science, Nagoya University, 
      Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602 Japan.
AD  - JST, ERATO Higashiyama Live-Holonics Project, Nagoya University, Furo-cho, 
      Chikusa-ku, Nagoya, Aichi, 464-8602 Japan.
FAU - Yamada, Tomomi
AU  - Yamada T
AD  - Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, 
      Chikusa-ku, Nagoya, Aichi, 464-8601 Japan.
FAU - Nishiwaki-Ohkawa, Taeko
AU  - Nishiwaki-Ohkawa T
AD  - Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, 
      Chikusa-ku, Nagoya, Aichi, 464-8601 Japan.
AD  - Laboratory of Animal Physiology, Department of Applied Molecular Biosciences, 
      Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, 
      Chikusa-ku, Nagoya, Aichi, 464-8601 Japan.
FAU - Kadofusa, Naoya
AU  - Kadofusa N
AD  - Laboratory of Animal Physiology, Department of Applied Molecular Biosciences, 
      Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, 
      Chikusa-ku, Nagoya, Aichi, 464-8601 Japan.
FAU - Kimata, Yusuke
AU  - Kimata Y
AD  - Division of Biological Science, Graduate School of Science, Nagoya University, 
      Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602 Japan.
FAU - Kuwata, Keiko
AU  - Kuwata K
AD  - Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, 
      Chikusa-ku, Nagoya, Aichi, 464-8601 Japan.
FAU - Umeda, Masaaki
AU  - Umeda M
AD  - Laboratory of Plant Growth Regulation, Graduate School of Biological Sciences, 
      Nara Institute of Science and Technology (NAIST), Takayama-cho 8916-5, Ikoma, 
      Nara, 630-0192 Japan.
AD  - JST, CREST, Takayama-cho 8916-5, Ikoma, Nara, 630-0192 Japan.
FAU - Ueda, Minako
AU  - Ueda M
AD  - Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, 
      Chikusa-ku, Nagoya, Aichi, 464-8601 Japan m-ueda@itbm.nagoya-u.ac.jp 
      mnambo@itbm.nagoya-u.ac.jp.
AD  - Division of Biological Science, Graduate School of Science, Nagoya University, 
      Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602 Japan.
AD  - These authors contributed equally to this work.
LA  - eng
PT  - Journal Article
DEP - 20160812
PL  - Japan
TA  - Plant Cell Physiol
JT  - Plant & cell physiology
JID - 9430925
RN  - 0 (Benzhydryl Compounds)
RN  - K3E387I0BC (diphenylmethane)
SB  - IM
MH  - Arabidopsis/*cytology
MH  - Benzhydryl Compounds/chemistry
MH  - Cell Cycle
MH  - *Cell Division
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Chemistry Techniques, Synthetic/*methods
MH  - HeLa Cells
MH  - High-Throughput Screening Assays
MH  - Humans
MH  - Molecular Imaging/*methods
MH  - Organ Specificity
MH  - Nicotiana/*cytology
OTO - NOTNLM
OT  - Arabidopsis thaliana
OT  - Cell division inhibitor
OT  - Live-cell imaging
OT  - Organic chemistry
OT  - Tobacco BY-2 cell
OT  - Triarylmethane
EDAT- 2016/08/16 06:00
MHDA- 2017/03/28 06:00
CRDT- 2016/08/14 06:00
PHST- 2016/05/20 00:00 [received]
PHST- 2016/08/02 00:00 [accepted]
PHST- 2016/08/16 06:00 [pubmed]
PHST- 2017/03/28 06:00 [medline]
PHST- 2016/08/14 06:00 [entrez]
AID - pcw140 [pii]
AID - 10.1093/pcp/pcw140 [doi]
PST - ppublish
SO  - Plant Cell Physiol. 2016 Nov;57(11):2255-2268. doi: 10.1093/pcp/pcw140. Epub 2016 
      Aug 12.