PMID- 2752130
OWN - NLM
STAT- MEDLINE
DCOM- 19890830
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 74
IP  - 1
DP  - 1989 Jul
TI  - Hematopoiesis on cellulose ester membranes. XI. Induction of new bone and a 
      hematopoietic microenvironment by matrix factors secreted by marrow stromal 
      cells.
PG  - 66-70
AB  - Cellulose ester membranes (CEM) were coated with stromal cells from bone marrow 
      (BM) or bone and implanted intraperitoneally (IP) in CAF1 mice for intervals of 1 
      to 6 months. Previous studies indicated that matrix factors [glycoproteins (GPs), 
      proteoglycans (PGs), and glycosaminoglycans (GAGs)] were secreted by the 
      regenerating stromal cells and adsorbed by the CEM. After 1 to 6 months, the CEMs 
      were removed, scraped free of adherent cells, and irradiated in vitro with 40 Gy. 
      The scraped and irradiated CEMs were then reimplanted IP or subcutaneously (SC) 
      for periods of 1 to 6 months in secondary syngeneic murine hosts. They were then 
      removed for histologic study. CEMs reimplanted in SC sites developed bone and 
      hematopoiesis as early as 1 month after implantation. Maximum hematopoiesis and 
      bone formation was observed after 3 months. CEMs coated during the initial 
      implantation with bone-derived stromal cells contained more bone and 
      hematopoietic cells than did CEMs coated with marrow-derived stromal cells after 
      SC implementation. Neither the CEMs coated with bone stromal cells nor those 
      coated with marrow stromal cells developed new bone or trilineal hematopoiesis 
      after being implanted IP. A few CEMs contained small foci of granulopoiesis only. 
      We conclude that noncellular matrix substances deposited on CEMs by bone, and to 
      a lesser degree by marrow cells, can induce prestromal cells in the SC tissues to 
      produce a microenvironment suitable for trilineal hematopoiesis.
FAU - Knospe, W H
AU  - Knospe WH
AD  - Department of Medicine, Rush-Presbyterian-St Luke's Medical Center, Chicago IL 
      60612.
FAU - Husseini, S G
AU  - Husseini SG
FAU - Fried, W
AU  - Fried W
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Membranes, Artificial)
RN  - 9004-34-6 (Cellulose)
SB  - IM
MH  - Animals
MH  - *Bone Marrow Cells
MH  - Bone and Bones/*cytology
MH  - Cellulose
MH  - Extracellular Matrix/physiology
MH  - *Hematopoiesis
MH  - In Vitro Techniques
MH  - Membranes, Artificial
MH  - Mice
EDAT- 1989/07/01 00:00
MHDA- 1989/07/01 00:01
CRDT- 1989/07/01 00:00
PHST- 1989/07/01 00:00 [pubmed]
PHST- 1989/07/01 00:01 [medline]
PHST- 1989/07/01 00:00 [entrez]
AID - S0006-4971(20)77322-2 [pii]
PST - ppublish
SO  - Blood. 1989 Jul;74(1):66-70.