PMID- 27521783
OWN - NLM
STAT- MEDLINE
DCOM- 20170111
LR  - 20181202
IS  - 1873-2976 (Electronic)
IS  - 0960-8524 (Linking)
VI  - 219
DP  - 2016 Nov
TI  - The near-ideal catalytic property of Candida antarctica lipase A to highly 
      concentrate n-3 polyunsaturated fatty acids in monoacylglycerols via one-step 
      ethanolysis of triacylglycerols.
PG  - 466-478
LID - S0960-8524(16)31128-2 [pii]
LID - 10.1016/j.biortech.2016.08.007 [doi]
AB  - Declining quantity/quality of available n-3 polyunsaturated fatty acids (n-3 
      PUFAs) resources demand innovative technology to concentrate n-3 PUFAs from low 
      quality oils into value-added products/health-beneficial ingredients rich in n-3 
      PUFAs. This work proposed the catalytic property and specificity of an ideal 
      enzyme required to tackle this task and identified Candida antarctica lipase A 
      (CAL-A) is such a near-ideal enzyme in practice, which concentrates n-3 PUFAs 
      from 25% to 27% in oils to a theoretically closer value 90% in monoacylglycerols 
      (MAGs) via one-step enzymatic ethanolysis. Non-regiospecificity and high non-n-3 
      PUFAs preference of CAL-A are the catalytic feature to selectively cleave non-n-3 
      PUFAs in all 3 positions of triacylglycerols (TAGs); while high ethanol/TAGs 
      ratio, low operation temperature and high tolerance to polar ethanol are 
      essential conditions beyond biocatalyst itself. C-13 Nuclear magnetic resonance 
      ((13)C NMR) analysis and competitive factor estimation verified the hypothesis 
      and confirmed the plausible suggestion of catalytic mechanism of CAL-A.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - He, Yongjin
AU  - He Y
AD  - College of Life Science, Fujian Normal University, Fuzhou 350117, China; 
      Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, 
      Denmark.
FAU - Li, Jingbo
AU  - Li J
AD  - Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, 
      Denmark.
FAU - Kodali, Sitharam
AU  - Kodali S
AD  - Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, 
      Denmark.
FAU - Chen, Bilian
AU  - Chen B
AD  - College of Life Science, Fujian Normal University, Fuzhou 350117, China; 
      Engineering Research Center of Industrial Microbiology of Ministry of Education, 
      Fujian Normal University, Fuzhou 350117, China. Electronic address: 
      chenbil@fjnu.edu.cn.
FAU - Guo, Zheng
AU  - Guo Z
AD  - Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, 
      Denmark.
LA  - eng
PT  - Journal Article
DEP - 20160805
PL  - England
TA  - Bioresour Technol
JT  - Bioresource technology
JID - 9889523
RN  - 0 (Fatty Acids, Omega-3)
RN  - 0 (Fungal Proteins)
RN  - 0 (Monoglycerides)
RN  - 0 (Triglycerides)
RN  - 3K9958V90M (Ethanol)
RN  - EC 3.1.1.3 (Lipase)
SB  - IM
MH  - Candida/enzymology
MH  - Catalysis
MH  - Ethanol/chemistry/metabolism
MH  - *Fatty Acids, Omega-3/chemistry/isolation & purification/metabolism
MH  - Fungal Proteins/metabolism
MH  - Lipase/*metabolism
MH  - *Monoglycerides/chemistry/metabolism
MH  - *Triglycerides/chemistry/metabolism
OTO - NOTNLM
OT  - Candida antarctica lipase A (CAL-A)
OT  - Ethanolysis
OT  - Fish oil
OT  - Microalgae oil
OT  - Monoacylglycerols (MAGs)
OT  - n-3 polyunsaturated fatty acids (n-3 PUFAs)
EDAT- 2016/08/16 06:00
MHDA- 2017/01/12 06:00
CRDT- 2016/08/14 06:00
PHST- 2016/05/31 00:00 [received]
PHST- 2016/08/01 00:00 [revised]
PHST- 2016/08/03 00:00 [accepted]
PHST- 2016/08/14 06:00 [entrez]
PHST- 2016/08/16 06:00 [pubmed]
PHST- 2017/01/12 06:00 [medline]
AID - S0960-8524(16)31128-2 [pii]
AID - 10.1016/j.biortech.2016.08.007 [doi]
PST - ppublish
SO  - Bioresour Technol. 2016 Nov;219:466-478. doi: 10.1016/j.biortech.2016.08.007. 
      Epub 2016 Aug 5.