PMID- 27521994
OWN - NLM
STAT- MEDLINE
DCOM- 20170208
LR  - 20170208
IS  - 1873-2933 (Electronic)
IS  - 0009-9120 (Linking)
VI  - 49
IP  - 16-17
DP  - 2016 Nov
TI  - Elevated methylation of CMTM3 promoter in the male laryngeal squamous cell 
      carcinoma patients.
PG  - 1278-1282
LID - S0009-9120(16)30181-3 [pii]
LID - 10.1016/j.clinbiochem.2016.08.002 [doi]
AB  - OBJECTIVE: CKLF-like MARVEL transmembrane domain containing 3 (CMTM3), as a tumor 
      suppressor gene, plays an important role in the suppression of cell growth and 
      apoptosis. The goal of our study is to investigate the association between CMTM3 
      promoter methylation and laryngeal squamous cell carcinoma (LSCC). DESIGN AND 
      METHODS: Using the bisulfite pyrosequencing technology, DNA methylation levels of 
      seven CpG sites in CMTM3 promoter are measured in tumor tissues and their 
      adjacent tissues of 76 male LSCC patients. RESULTS: Our results reveal a 
      significantly elevated promoter methylation of CMTM3 in tumor tissues compared 
      with their adjacent tissues (P<0.001). A breakdown analysis by age shows that 
      significant association of CMTM3 promoter methylation with cancer risk is 
      specific to the LSCC patients older than 55years (P<0.001) but not in the younger 
      patients (P=0.305). Moreover, the association is only observed in the LSCC 
      patients with smoking behavior (P=0.001). Breakdown analysis also shows that 
      CMTM3 promoter methylation is associated with cancer risk among patients with 
      stage I LSCC (P<0.001). CONCLUSION: In conclusion, our study indicates that 
      elevated CMTM3 methylation is a risk factor in male LSCC patients, especially in 
      the patients with age over 55years and with smoking behavior.
CI  - Copyright (c) 2016 The Canadian Society of Clinical Chemists. Published by Elsevier 
      Inc. All rights reserved.
FAU - Shen, Zhisen
AU  - Shen Z
AD  - Department of Otorhinolaryngology (Head and Neck Surgery), Ningbo Medical Center 
      Lihuili Hospital, Ningbo, Zhejiang 315040, China. Electronic address: 
      szs7216@163.com.
FAU - Chen, Xiaoying
AU  - Chen X
AD  - Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 
      315211, China.
FAU - Li, Qun
AU  - Li Q
AD  - Department of Otorhinolaryngology (Head and Neck Surgery), Ningbo Medical Center 
      Lihuili Hospital, Ningbo, Zhejiang 315040, China.
FAU - Zhou, Chongchang
AU  - Zhou C
AD  - Department of Otorhinolaryngology (Head and Neck Surgery), Ningbo Medical Center 
      Lihuili Hospital, Ningbo, Zhejiang 315040, China; Medical Genetics Center, School 
      of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China.
FAU - Xu, Yan
AU  - Xu Y
AD  - Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 
      315211, China.
FAU - Yu, Rui
AU  - Yu R
AD  - Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 
      315211, China.
FAU - Ye, Huadan
AU  - Ye H
AD  - Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 
      315211, China.
FAU - Li, Jinyun
AU  - Li J
AD  - Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 
      315211, China.
FAU - Duan, Shiwei
AU  - Duan S
AD  - Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 
      315211, China. Electronic address: duanshiwei@nbu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20160810
PL  - United States
TA  - Clin Biochem
JT  - Clinical biochemistry
JID - 0133660
RN  - 0 (CMTM3 protein, human)
RN  - 0 (Chemokines)
RN  - 0 (MARVEL Domain-Containing Proteins)
SB  - IM
MH  - Base Sequence
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Chemokines/*genetics
MH  - *DNA Methylation
MH  - HEK293 Cells
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics
MH  - MARVEL Domain-Containing Proteins/*genetics
MH  - Male
MH  - *Promoter Regions, Genetic
OTO - NOTNLM
OT  - CMTM3
OT  - DNA methylation
OT  - Laryngeal carcinoma
OT  - Male
OT  - Promoter
EDAT- 2016/10/23 06:00
MHDA- 2017/02/09 06:00
CRDT- 2016/08/14 06:00
PHST- 2016/02/21 00:00 [received]
PHST- 2016/06/28 00:00 [revised]
PHST- 2016/08/06 00:00 [accepted]
PHST- 2016/10/23 06:00 [pubmed]
PHST- 2017/02/09 06:00 [medline]
PHST- 2016/08/14 06:00 [entrez]
AID - S0009-9120(16)30181-3 [pii]
AID - 10.1016/j.clinbiochem.2016.08.002 [doi]
PST - ppublish
SO  - Clin Biochem. 2016 Nov;49(16-17):1278-1282. doi: 
      10.1016/j.clinbiochem.2016.08.002. Epub 2016 Aug 10.