PMID- 27524058
OWN - NLM
STAT- MEDLINE
DCOM- 20170406
LR  - 20180320
IS  - 1873-0191 (Electronic)
IS  - 0928-4931 (Linking)
VI  - 68
DP  - 2016 Nov 1
TI  - A label-free aptasensor based on polyethyleneimine wrapped carbon nanotubes in 
      situ formed gold nanoparticles as signal probe for highly sensitive detection of 
      dopamine.
PG  - 585-593
LID - S0928-4931(16)30510-0 [pii]
LID - 10.1016/j.msec.2016.05.077 [doi]
AB  - Herein, a highly sensitive and selective aptamer biosensor for quantitative 
      detection of a model target, dopamine (DA), was developed by using a gold (Au) 
      electrode modified with highly dispersed gold nanoparticles (AuNPs) and 
      acid-oxidized carbon nanotubes (CNTs-COOH) functionalized with polyethyleneimine 
      (PEI). Amine-terminated12-mercaptureprobe (ssDNA1) as a capture probe and 
      specific DA-aptamer (ssDNA2) as a detection probe was immobilized on the surface 
      of a modified electrode via the formation of covalent amide bond and 
      hybridization, respectively. Methylene blue (MB) was used as the redox probe, 
      which was intercalated into the aptamer through the specific interaction with its 
      guanine bases. In the presence of DA, the interaction between aptamer and DA 
      displaced the MB from the electrode surface, rendering a lowered electrochemical 
      signal attributed to decreased amount of adsorbed MB. The developed 
      electrochemical DA aptasensor showed a good linear response to DA from 5 to 300nM 
      with detection limit of 2.1nM. The biosensor also exhibited satisfactory 
      selectivity and could be successfully used to detect DA in blood serum sample.
CI  - Copyright (c) 2016. Published by Elsevier B.V.
FAU - Azadbakht, Azadeh
AU  - Azadbakht A
AD  - Department of Chemistry, Khorramabad Branch, Islamic Azad University, 
      Khorramabad, Iran. Electronic address: Azadbakht.a@gmail.com.
FAU - Roushani, Mahmoud
AU  - Roushani M
AD  - Department of Chemistry, Ilam University, Ilam, Iran.
FAU - Abbasi, Amir Reza
AU  - Abbasi AR
AD  - Department of Chemistry, Khorramabad Branch, Islamic Azad University, 
      Khorramabad, Iran.
FAU - Menati, Saeid
AU  - Menati S
AD  - Department of Chemistry, Khorramabad Branch, Islamic Azad University, 
      Khorramabad, Iran.
FAU - Derikvand, Zohreh
AU  - Derikvand Z
AD  - Department of Chemistry, Khorramabad Branch, Islamic Azad University, 
      Khorramabad, Iran.
LA  - eng
PT  - Journal Article
DEP - 20160520
PL  - Netherlands
TA  - Mater Sci Eng C Mater Biol Appl
JT  - Materials science & engineering. C, Materials for biological applications
JID - 101484109
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Nanotubes, Carbon)
RN  - 7440-57-5 (Gold)
RN  - 9002-98-6 (Polyethyleneimine)
RN  - T42P99266K (Methylene Blue)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - *Biosensing Techniques
MH  - Dopamine/*analysis
MH  - Gold/*chemistry
MH  - Metal Nanoparticles/*chemistry
MH  - Methylene Blue/chemistry
MH  - Nanotubes, Carbon/*chemistry
MH  - Polyethyleneimine/*chemistry
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - Aptasensor
OT  - Dopamine
OT  - Gold nanoparticles
OT  - Polyethyleneimine
EDAT- 2016/08/16 06:00
MHDA- 2017/04/07 06:00
CRDT- 2016/08/16 06:00
PHST- 2016/02/01 00:00 [received]
PHST- 2016/04/10 00:00 [revised]
PHST- 2016/05/18 00:00 [accepted]
PHST- 2016/08/16 06:00 [entrez]
PHST- 2016/08/16 06:00 [pubmed]
PHST- 2017/04/07 06:00 [medline]
AID - S0928-4931(16)30510-0 [pii]
AID - 10.1016/j.msec.2016.05.077 [doi]
PST - ppublish
SO  - Mater Sci Eng C Mater Biol Appl. 2016 Nov 1;68:585-593. doi: 
      10.1016/j.msec.2016.05.077. Epub 2016 May 20.