PMID- 27524971
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160815
LR  - 20200930
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 7
DP  - 2016
TI  - A Novel Chip for Cyclic Stretch and Intermittent Hypoxia Cell Exposures Mimicking 
      Obstructive Sleep Apnea.
PG  - 319
LID - 10.3389/fphys.2016.00319 [doi]
LID - 319
AB  - Intermittent hypoxia (IH), a hallmark of obstructive sleep apnea (OSA), plays a 
      critical role in the pathogenesis of OSA-associated morbidities, especially in 
      the cardiovascular and respiratory systems. Oxidative stress and inflammation 
      induced by IH are suggested as main contributors of end-organ dysfunction in OSA 
      patients and animal models. Since the molecular mechanisms underlying these in 
      vivo pathological responses remain poorly understood, implementation of 
      experimental in vitro cell-based systems capable of inducing high-frequency IH 
      would be highly desirable. Here, we describe the design, fabrication, and 
      validation of a versatile chip for subjecting cultured cells to fast changes in 
      gas partial pressure and to cyclic stretch. The chip is fabricated with 
      polydimethylsiloxane (PDMS) and consists of a cylindrical well-covered by a thin 
      membrane. Cells cultured on top of the membrane can be subjected to fast changes 
      in oxygen concentration (equilibrium time ~6 s). Moreover, cells can be subjected 
      to cyclic stretch at cardiac or respiratory frequencies independently or 
      simultaneously. Rat bone marrow-derived mesenchymal stem cells (MSCs) exposed to 
      IH mimicking OSA and cyclic stretch at cardiac frequencies revealed that 
      hypoxia-inducible factor 1alpha (HIF-1alpha) expression was increased in response to both 
      stimuli. Thus, the chip provides a versatile tool for the study of cellular 
      responses to cyclical hypoxia and stretch.
FAU - Campillo, Noelia
AU  - Campillo N
AD  - Unitat de Biofisica i Bioenginyeria, Facultat de Medicina, Universitat de 
      BarcelonaBarcelona, Spain; Cellular and Respiratory Biomechanics, Institute for 
      Bioengineering of CataloniaBarcelona, Spain; Centro de Investigacion Biomedica en 
      Red de Enfermedades RespiratoriasMadrid, Spain.
FAU - Jorba, Ignasi
AU  - Jorba I
AD  - Unitat de Biofisica i Bioenginyeria, Facultat de Medicina, Universitat de 
      BarcelonaBarcelona, Spain; Cellular and Respiratory Biomechanics, Institute for 
      Bioengineering of CataloniaBarcelona, Spain; Centro de Investigacion Biomedica en 
      Red de Enfermedades RespiratoriasMadrid, Spain.
FAU - Schaedel, Laura
AU  - Schaedel L
AD  - Unitat de Biofisica i Bioenginyeria, Facultat de Medicina, Universitat de 
      BarcelonaBarcelona, Spain; Cellular and Respiratory Biomechanics, Institute for 
      Bioengineering of CataloniaBarcelona, Spain.
FAU - Casals, Blai
AU  - Casals B
AD  - Unitat de Biofisica i Bioenginyeria, Facultat de Medicina, Universitat de 
      BarcelonaBarcelona, Spain; Cellular and Respiratory Biomechanics, Institute for 
      Bioengineering of CataloniaBarcelona, Spain.
FAU - Gozal, David
AU  - Gozal D
AD  - Biological Sciences Division, Department of Pediatrics, Pritzker School of 
      Medicine, The University of Chicago Chicago, IL, USA.
FAU - Farre, Ramon
AU  - Farre R
AD  - Unitat de Biofisica i Bioenginyeria, Facultat de Medicina, Universitat de 
      BarcelonaBarcelona, Spain; Centro de Investigacion Biomedica en Red de 
      Enfermedades RespiratoriasMadrid, Spain; Institut d'Investigacions Biomediques 
      August Pi i SunyerBarcelona, Spain.
FAU - Almendros, Isaac
AU  - Almendros I
AD  - Unitat de Biofisica i Bioenginyeria, Facultat de Medicina, Universitat de 
      BarcelonaBarcelona, Spain; Centro de Investigacion Biomedica en Red de 
      Enfermedades RespiratoriasMadrid, Spain; Institut d'Investigacions Biomediques 
      August Pi i SunyerBarcelona, Spain.
FAU - Navajas, Daniel
AU  - Navajas D
AD  - Unitat de Biofisica i Bioenginyeria, Facultat de Medicina, Universitat de 
      BarcelonaBarcelona, Spain; Cellular and Respiratory Biomechanics, Institute for 
      Bioengineering of CataloniaBarcelona, Spain; Centro de Investigacion Biomedica en 
      Red de Enfermedades RespiratoriasMadrid, Spain.
LA  - eng
PT  - Journal Article
DEP - 20160729
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC4965455
OTO - NOTNLM
OT  - cell stretch
OT  - hypoxia-inducible factor
OT  - intermittent hypoxia
OT  - lab-on-a-chip
OT  - obstructive sleep apnea
EDAT- 2016/08/16 06:00
MHDA- 2016/08/16 06:01
PMCR- 2016/07/29
CRDT- 2016/08/16 06:00
PHST- 2016/05/13 00:00 [received]
PHST- 2016/07/13 00:00 [accepted]
PHST- 2016/08/16 06:00 [entrez]
PHST- 2016/08/16 06:00 [pubmed]
PHST- 2016/08/16 06:01 [medline]
PHST- 2016/07/29 00:00 [pmc-release]
AID - 10.3389/fphys.2016.00319 [doi]
PST - epublish
SO  - Front Physiol. 2016 Jul 29;7:319. doi: 10.3389/fphys.2016.00319. eCollection 
      2016.