PMID- 27525319
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160815
LR  - 20200928
IS  - 2476-4108 (Print)
IS  - 2476-3772 (Electronic)
IS  - 2476-3772 (Linking)
VI  - 14
IP  - 6
DP  - 2016 Jun
TI  - The effect of heracleum persicum (Golpar) oil and alcoholic extracts on sperm 
      parameters and chromatin quality in mice.
PG  - 365-70
AB  - BACKGROUND: Evaluating the significance and the effects of plant-derived drugs on 
      laboratory animal's fertility was recognized. There was antioxidant activity 
      reported from Heracleum persicum (Golpar). OBJECTIVE: Current study aims to study 
      the antioxidant effect of Golpar extracts on sperm parameters and chromatin 
      quality in mice. MATERIALS AND METHODS: Eighteen adult male mice were divided to 
      3 groups (10 wk old, 35 gr weight): group1 received hydro alcoholic extract (1000 
      mg/kg, ip), group 2 received oil extract (200 mg/kg, ip) and group 3 serving as 
      the sham control group that received sterile water. Finally, left cauda 
      epididymis of each animal was dissected and sperm analysis was done accordingly. 
      To asses sperm chromatin and DNA quality, we used aniline blue (AB), toluidine 
      blue (TB), chromomycin A3 (CMA3) and acridine orange (AO) staining. RESULTS: 
      Progressive and non-progressive sperm motility were significantly increased in 
      group 1 in comparison with group 3 (p=0.032). There was an increasing trend in 
      progressive sperm motility and decreasing trend in non-progressive sperm motility 
      in group 2 in comparison with group 3, but the differences were not significant 
      (p=0.221 and p=0.144, respectively). According to the sperm chromatin quality, 
      the results of TB and AO tests revealed significant differences (p=0.004, 
      p=0.000, respectively) between those groups and showed that the extracts of 
      Golpar cause DNA damage, but no differences can be observed between them in AB 
      and CMA3 staining (p>0.05). CONCLUSION: The results showed that Heracleum 
      persicum extracts may improve sperm motility. Also, it has harmful effects on 
      sperm chromatin condensation and DNA integrity in mice.
FAU - Taghizabet, Neda
AU  - Taghizabet N
AD  - Department of Biology and Anatomical Sciences, Shahid Sadoughi University of 
      Medical Sciences, Yazd, Iran.
FAU - Mangoli, Esmat
AU  - Mangoli E
AD  - Research and Clinical Center for Infertility, Shahid Sadoughi University of 
      Medical Sciences, Yazd, Iran.
FAU - Anbari, Fatemeh
AU  - Anbari F
AD  - Research and Clinical Center for Infertility, Shahid Sadoughi University of 
      Medical Sciences, Yazd, Iran.
FAU - Masoodi, Seyed Ali
AU  - Masoodi SA
AD  - School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
FAU - Talebi, Ali Reza
AU  - Talebi AR
AD  - Research and Clinical Center for Infertility, Shahid Sadoughi University of 
      Medical Sciences, Yazd, Iran.
FAU - Mazrooei, Malihe
AU  - Mazrooei M
AD  - School of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Int J Reprod Biomed
JT  - International journal of reproductive biomedicine
JID - 101679102
PMC - PMC4971554
OTO - NOTNLM
OT  - Chromatin
OT  - DNA integrity
OT  - Heracleum persicum extracts
OT  - Mice
OT  - Sperm
EDAT- 2016/08/16 06:00
MHDA- 2016/08/16 06:01
PMCR- 2016/06/01
CRDT- 2016/08/16 06:00
PHST- 2016/08/16 06:00 [entrez]
PHST- 2016/08/16 06:00 [pubmed]
PHST- 2016/08/16 06:01 [medline]
PHST- 2016/06/01 00:00 [pmc-release]
AID - ijrb-14-365 [pii]
PST - ppublish
SO  - Int J Reprod Biomed. 2016 Jun;14(6):365-70.