PMID- 27527768
OWN - NLM
STAT- MEDLINE
DCOM- 20170421
LR  - 20220408
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 192
DP  - 2016 Aug 30
TI  - Inactivated porcine reproductive and respiratory syndrome virus vaccine 
      adjuvanted with Montanide Gel 01 ST elicits virus-specific cross-protective 
      inter-genotypic response in piglets.
PG  - 81-89
LID - S0378-1135(16)30177-8 [pii]
LID - 10.1016/j.vetmic.2016.06.014 [doi]
AB  - The efficacy of a novel BEI-inactivated porcine reproductive and respiratory 
      syndrome virus (PRRSV) candidate vaccine in pigs, developed at RIBSP Republic of 
      Kazakhstan and delivered with an adjuvant Montanide Gel 01 ST (D/KV/ADJ) was 
      compared with a commercial killed PRRSV vaccine (NVDC-JXA1, C/KV/ADJ) used widely 
      in swine herds of the Republic of Kazakhstan. Clinical parameters (body 
      temperature and respiratory disease scores), virological and immunological 
      profiles [ELISA and virus neutralizing (VN) antibody titers], macroscopic lung 
      lesions and viral load in the lungs (quantitative real-time PCR and cell culture 
      assay) were assessed in vaccinated and both genotype 1 and 2 PRRSV challenged 
      pigs. Our results showed that the commercial vaccine failed to protect pigs 
      adequately against the clinical disease, viremia and lung lesions caused by the 
      challenged field isolates, Kazakh strains of PRRSV type 1 and type 2 genotypes. 
      In contrast, clinical protection, absence of viremia and lung lesions in D/KV/ADJ 
      vaccinated pigs was associated with generation of VN antibodies in both 
      homologous vaccine strain LKZ/2010 (PRRSV type 2) and a heterogeneous type 1 
      PRRSV strain (CM/08) challenged pigs. Thus, our data indicated the induction of 
      cross-protective VN antibodies by D/KV/ADJ vaccine, and importantly demonstrated 
      that an inactivated PRRSV vaccine could also induce cross-protective response 
      across the viral genotype.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Tabynov, Kairat
AU  - Tabynov K
AD  - Research Institute for Biological Safety Problems (RIBSP), Science Committee, 
      Ministry of Education and Science of the Republic of Kazakhstan, Zhambylskaya 
      oblast, Kordaiskiy rayon, Gvardeiskiy 080409, Kazakhstan. Electronic address: 
      tabynov777@mail.ru.
FAU - Sansyzbay, Abylay
AU  - Sansyzbay A
AD  - Research Institute for Biological Safety Problems (RIBSP), Science Committee, 
      Ministry of Education and Science of the Republic of Kazakhstan, Zhambylskaya 
      oblast, Kordaiskiy rayon, Gvardeiskiy 080409, Kazakhstan.
FAU - Tulemissova, Zhanara
AU  - Tulemissova Z
AD  - Faculty of Veterinary Science, Department of Biological Safety, Kazakh National 
      Agrarian University (KazNAU), Almaty 050010, Kazakhstan.
FAU - Tabynov, Kaissar
AU  - Tabynov K
AD  - Research Institute for Biological Safety Problems (RIBSP), Science Committee, 
      Ministry of Education and Science of the Republic of Kazakhstan, Zhambylskaya 
      oblast, Kordaiskiy rayon, Gvardeiskiy 080409, Kazakhstan.
FAU - Dhakal, Santosh
AU  - Dhakal S
AD  - Food Animal Health Research Program, Ohio Agricultural Research and Development 
      Center, Department of Veterinary Preventive Medicine, The Ohio State University 
      (OSU), Wooster, OH 44691, USA.
FAU - Samoltyrova, Aigul
AU  - Samoltyrova A
AD  - Research Institute for Biological Safety Problems (RIBSP), Science Committee, 
      Ministry of Education and Science of the Republic of Kazakhstan, Zhambylskaya 
      oblast, Kordaiskiy rayon, Gvardeiskiy 080409, Kazakhstan.
FAU - Renukaradhya, Gourapura J
AU  - Renukaradhya GJ
AD  - Food Animal Health Research Program, Ohio Agricultural Research and Development 
      Center, Department of Veterinary Preventive Medicine, The Ohio State University 
      (OSU), Wooster, OH 44691, USA.
FAU - Mambetaliyev, Muratbay
AU  - Mambetaliyev M
AD  - Research Institute for Biological Safety Problems (RIBSP), Science Committee, 
      Ministry of Education and Science of the Republic of Kazakhstan, Zhambylskaya 
      oblast, Kordaiskiy rayon, Gvardeiskiy 080409, Kazakhstan.
LA  - eng
PT  - Journal Article
DEP - 20160701
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Vaccines, Inactivated)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Adjuvants, Immunologic/*pharmacology
MH  - Animals
MH  - Antibodies, Viral/*blood
MH  - Antibody Formation/immunology
MH  - Antibody Specificity
MH  - Genotype
MH  - Porcine Reproductive and Respiratory Syndrome/blood/*prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Swine
MH  - Vaccines, Inactivated
MH  - Viral Vaccines/*immunology
MH  - Viremia
PMC - PMC7111292
OTO - NOTNLM
OT  - Binary ethylenimine
OT  - Cross-protection
OT  - Killed vaccines
OT  - Neutralizing antibodies
OT  - PRRSV
OT  - Polymeric adjuvant
EDAT- 2016/08/17 06:00
MHDA- 2017/04/22 06:00
PMCR- 2016/07/01
CRDT- 2016/08/17 06:00
PHST- 2016/04/21 00:00 [received]
PHST- 2016/06/29 00:00 [revised]
PHST- 2016/06/30 00:00 [accepted]
PHST- 2016/08/17 06:00 [entrez]
PHST- 2016/08/17 06:00 [pubmed]
PHST- 2017/04/22 06:00 [medline]
PHST- 2016/07/01 00:00 [pmc-release]
AID - S0378-1135(16)30177-8 [pii]
AID - 10.1016/j.vetmic.2016.06.014 [doi]
PST - ppublish
SO  - Vet Microbiol. 2016 Aug 30;192:81-89. doi: 10.1016/j.vetmic.2016.06.014. Epub 
      2016 Jul 1.