PMID- 27529188
OWN - NLM
STAT- MEDLINE
DCOM- 20171030
LR  - 20210620
IS  - 2050-084X (Electronic)
IS  - 2050-084X (Linking)
VI  - 5
DP  - 2016 Aug 16
TI  - Structural characterization of encapsulated ferritin provides insight into iron 
      storage in bacterial nanocompartments.
LID - e18972 [pii]
LID - 10.7554/eLife.18972 [doi]
AB  - Ferritins are ubiquitous proteins that oxidise and store iron within a protein 
      shell to protect cells from oxidative damage. We have characterized the structure 
      and function of a new member of the ferritin superfamily that is sequestered 
      within an encapsulin capsid. We show that this encapsulated ferritin (EncFtn) has 
      two main alpha helices, which assemble in a metal dependent manner to form a 
      ferroxidase center at a dimer interface. EncFtn adopts an open decameric 
      structure that is topologically distinct from other ferritins. While EncFtn acts 
      as a ferroxidase, it cannot mineralize iron. Conversely, the encapsulin shell 
      associates with iron, but is not enzymatically active, and we demonstrate that 
      EncFtn must be housed within the encapsulin for iron storage. This encapsulin 
      nanocompartment is widely distributed in bacteria and archaea and represents a 
      distinct class of iron storage system, where the oxidation and mineralization of 
      iron are distributed between two proteins.
FAU - He, Didi
AU  - He D
AUID- ORCID: 0000-0002-3360-9352
AD  - Institute of Quantitative Biology, Biochemistry and Biotechnology, School of 
      Biological Sciences, The University of Edinburgh, Edinburgh, United Kingdom.
FAU - Hughes, Sam
AU  - Hughes S
AD  - The School of Chemistry, The University of Edinburgh, Edinburgh, United Kingdom.
FAU - Vanden-Hehir, Sally
AU  - Vanden-Hehir S
AD  - The School of Chemistry, The University of Edinburgh, Edinburgh, United Kingdom.
FAU - Georgiev, Atanas
AU  - Georgiev A
AD  - Institute of Quantitative Biology, Biochemistry and Biotechnology, School of 
      Biological Sciences, The University of Edinburgh, Edinburgh, United Kingdom.
FAU - Altenbach, Kirsten
AU  - Altenbach K
AD  - Institute of Quantitative Biology, Biochemistry and Biotechnology, School of 
      Biological Sciences, The University of Edinburgh, Edinburgh, United Kingdom.
FAU - Tarrant, Emma
AU  - Tarrant E
AD  - Institute for Cell and Molecular Biosciences, Newcastle University, Newcasle upon 
      Tyne, United Kingdom.
FAU - Mackay, C Logan
AU  - Mackay CL
AD  - The School of Chemistry, The University of Edinburgh, Edinburgh, United Kingdom.
FAU - Waldron, Kevin J
AU  - Waldron KJ
AUID- ORCID: 0000-0002-5577-7357
AD  - Institute for Cell and Molecular Biosciences, Newcastle University, Newcasle upon 
      Tyne, United Kingdom.
FAU - Clarke, David J
AU  - Clarke DJ
AUID- ORCID: 0000-0002-3741-2952
AD  - The School of Chemistry, The University of Edinburgh, Edinburgh, United Kingdom.
FAU - Marles-Wright, Jon
AU  - Marles-Wright J
AUID- ORCID: 0000-0002-9156-3284
AD  - Institute of Quantitative Biology, Biochemistry and Biotechnology, School of 
      Biological Sciences, The University of Edinburgh, Edinburgh, United Kingdom.
AD  - School of Biology, Newcastle University, Newcastle upon Tyne, United Kingdom.
LA  - eng
GR  - WT_/Wellcome Trust/United Kingdom
GR  - 098375/Z/12/Z/WT_/Wellcome Trust/United Kingdom
GR  - BB/N005570/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160816
PL  - England
TA  - Elife
JT  - eLife
JID - 101579614
RN  - 9007-73-2 (Ferritins)
RN  - E1UOL152H7 (Iron)
RN  - EC 1.16.3.1 (Ceruloplasmin)
SB  - IM
MH  - Ceruloplasmin/chemistry/metabolism
MH  - Crystallography, X-Ray
MH  - Ferritins/*chemistry/*metabolism
MH  - Iron/*metabolism
MH  - Microscopy, Electron, Transmission
MH  - Models, Molecular
MH  - Protein Conformation
MH  - Protein Multimerization
MH  - Rhodospirillum rubrum/*enzymology/*metabolism
PMC - PMC5012862
OTO - NOTNLM
OT  - Rhodospirillum rubrum
OT  - biochemistry
OT  - biophysics
OT  - encapsulated ferritin
OT  - encapsulin
OT  - ferritin
OT  - structural biology
COIS- The authors declare that no competing interests exist.
EDAT- 2016/08/17 06:00
MHDA- 2017/10/31 06:00
PMCR- 2016/08/17
CRDT- 2016/08/17 06:00
PHST- 2016/06/24 00:00 [received]
PHST- 2016/08/14 00:00 [accepted]
PHST- 2016/08/17 06:00 [entrez]
PHST- 2016/08/17 06:00 [pubmed]
PHST- 2017/10/31 06:00 [medline]
PHST- 2016/08/17 00:00 [pmc-release]
AID - e18972 [pii]
AID - 18972 [pii]
AID - 10.7554/eLife.18972 [doi]
PST - epublish
SO  - Elife. 2016 Aug 16;5:e18972. doi: 10.7554/eLife.18972.