PMID- 27534563
OWN - NLM
STAT- MEDLINE
DCOM- 20171017
LR  - 20221207
IS  - 1437-1596 (Electronic)
IS  - 0937-9827 (Linking)
VI  - 131
IP  - 1
DP  - 2017 Jan
TI  - Deposition of diazepam and its metabolites in hair following a single dose of 
      diazepam.
PG  - 131-141
LID - 10.1007/s00414-016-1429-x [doi]
AB  - Only sporadic data are available on hair concentrations of diazepam and some of 
      its metabolites (nordazepam, oxazepam, and temazepam) following a single 
      controlled dose. The aim of this study was to investigate the deposition of 
      diazepam and its metabolites in human hair after eight healthy volunteers (four 
      women and four men, ages 24-26, East Asian) consumed 10 mg of diazepam. Hair was 
      collected from all volunteers 1 month after exposure, and also 2 months 
      post-exposure from men and 10 months post-exposure from women. Diazepam and the 
      complete metabolite profile, including oxazepam glucuronide and temazepam 
      glucuronide, were measured by ultra-high pressure liquid chromatography-tandem 
      mass spectrometry (UHPLC-MS/MS) with limits of quantifications (LOQs) of 
      0.5-2.5 pg/mg for diazepam, nordazepam, oxazepam, and temazepam, and of 10 pg/mg 
      for oxazepam glucuronide and temazepam glucuronide. There were no differences by 
      gender in the amounts of diazepam or metabolites found. The concentration of the 
      main metabolite nordazepam was consistently higher than that of diazepam at both 
      1 and 2 months after consumption. Oxazepam and temazepam traces were found in 
      some volunteers' hair, but the glucuronides were not detected. Diazepam and 
      nordazepam levels at 10 months post-exposure were extremely low (near the LOQ), 
      indicating drug loss by personal hygiene and physical handling. To our knowledge, 
      this is the first single-dose diazepam study using black hair and the first study 
      to include measurements of oxazepam glucuronide and temazepam glucuronide in 
      human hair.
FAU - Wang, Xin
AU  - Wang X
AD  - Section of Forensic Chemistry, Department of Forensic Medicine, Faculty of Health 
      and Medical Sciences, University of Copenhagen, Frederik V's Vej 11, 2100, 
      Copenhagen, Denmark. xinwangyao@163.com.
FAU - Johansen, Sys Stybe
AU  - Johansen SS
AD  - Section of Forensic Chemistry, Department of Forensic Medicine, Faculty of Health 
      and Medical Sciences, University of Copenhagen, Frederik V's Vej 11, 2100, 
      Copenhagen, Denmark.
FAU - Zhang, Yurong
AU  - Zhang Y
AUID- ORCID: 0000-0001-7674-7010
AD  - Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Institute of Forensic 
      Science, 803 Zhong Shan Bei Yi Road, Shanghai, 200083, People's Republic of 
      China. yr_zhang@126.com.
FAU - Jia, Jingying
AU  - Jia J
AD  - Central Laboratory, Shanghai Xuhui Central Hospital, Shanghai Clinical Center, 
      Chinese Academy of Sciences, Shanghai, People's Republic of China.
FAU - Rao, Yulan
AU  - Rao Y
AD  - Department of Forensic Medicine, School of Basic Medical Sciences, Fudan 
      University, Shanghai, People's Republic of China.
FAU - Jiang, Fengli
AU  - Jiang F
AD  - Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Institute of Forensic 
      Science, 803 Zhong Shan Bei Yi Road, Shanghai, 200083, People's Republic of 
      China.
AD  - Department of Forensic Medicine, School of Basic Medical Sciences, Fudan 
      University, Shanghai, People's Republic of China.
FAU - Linnet, Kristian
AU  - Linnet K
AD  - Section of Forensic Chemistry, Department of Forensic Medicine, Faculty of Health 
      and Medical Sciences, University of Copenhagen, Frederik V's Vej 11, 2100, 
      Copenhagen, Denmark.
LA  - eng
PT  - Journal Article
DEP - 20160817
PL  - Germany
TA  - Int J Legal Med
JT  - International journal of legal medicine
JID - 9101456
RN  - 0 (Hypnotics and Sedatives)
RN  - 26IK2C76NO (oxazepam glucuronide)
RN  - 3703-53-5 (temazepam glucuronide)
RN  - 67220MCM01 (Nordazepam)
RN  - 6GOW6DWN2A (Oxazepam)
RN  - CHB1QD2QSS (Temazepam)
RN  - Q3JTX2Q7TU (Diazepam)
SB  - IM
MH  - Adult
MH  - Asian People
MH  - Chromatography, Liquid
MH  - Diazepam/administration & dosage/*analysis
MH  - Female
MH  - Hair/*chemistry
MH  - Healthy Volunteers
MH  - Humans
MH  - Hypnotics and Sedatives/administration & dosage/*analysis
MH  - Male
MH  - Nordazepam/analysis
MH  - Oxazepam/analogs & derivatives/analysis
MH  - Tandem Mass Spectrometry
MH  - Temazepam/analogs & derivatives/analysis
MH  - Young Adult
OTO - NOTNLM
OT  - Diazepam
OT  - Glucuronides
OT  - Hair
OT  - Metabolites
OT  - Single dose
OT  - UHPLC-MS/MS
EDAT- 2016/08/19 06:00
MHDA- 2017/10/19 06:00
CRDT- 2016/08/19 06:00
PHST- 2016/03/02 00:00 [received]
PHST- 2016/07/26 00:00 [accepted]
PHST- 2016/08/19 06:00 [pubmed]
PHST- 2017/10/19 06:00 [medline]
PHST- 2016/08/19 06:00 [entrez]
AID - 10.1007/s00414-016-1429-x [pii]
AID - 10.1007/s00414-016-1429-x [doi]
PST - ppublish
SO  - Int J Legal Med. 2017 Jan;131(1):131-141. doi: 10.1007/s00414-016-1429-x. Epub 
      2016 Aug 17.