PMID- 27534746 OWN - NLM STAT- MEDLINE DCOM- 20171025 LR - 20181113 IS - 1755-8794 (Electronic) IS - 1755-8794 (Linking) VI - 9 Suppl 1 IP - Suppl 1 DP - 2016 Aug 12 TI - Major methylation alterations on the CpG markers of inflammatory immune associated genes after IVIG treatment in Kawasaki disease. PG - 37 LID - 10.1186/s12920-016-0197-2 [doi] LID - 37 AB - BACKGROUND: Kawasaki disease (KD) is an autoimmune disease preferentially attacking children younger than five years worldwide. So far, the principal treatment to KD is the administration of Intravenous immunoglobulin (IVIG). Although DNA methylation plays important regulation roles in diseases, few studies investigated the regulation roles of DNA methylation in KD. METHODS: In this study, we focused not only on the DNA methylation alterations resulted from KD onset but also on DNA methylation alterations resulted from IVIG administration. To do so, we investigated the effects of KD's onset and IVIG administration on CpG marker's methylation alterations. RESULTS: We first found that DNA methylation alterations reflecting disease onset or IVIG administration are contributed mainly by the CpG markers on autosomes. In addition, we also demonstrated that some CpG markers carry methylation alteration among samples, forcing the expression abundance of the downstream genes to be also altered and negatively correlated with methylation profile. Finally, compared with KD's onset, IVIG administration brings stronger impact on methylation alteration. And, such alterations were conducted mainly by hyper-methylating CpG markers, forcing the corresponding genes to keep lower expression levels. Moreover, the genes regulated by the altered CpG markers with IVIG administration are enriched in the pathways associated with inflammatory immune response. CONCLUSIONS: In summary, our result provides researchers with another way into the regulation mechanism through which IVIG represses excessive inflammatory responses. FAU - Li, Sung-Chou AU - Li SC AD - Genomics and Proteomics Core Laboratory, Department of Medical Research, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan. FAU - Chan, Wen-Ching AU - Chan WC AD - Center for Research Informatics, The University of Chicago, Chicago, IL, USA. FAU - Huang, Ying-Hsien AU - Huang YH AD - Kawasaki Disease Center, Kaohsiung Chang Gung Memorial Hospital, No.123, Dapi Rd, Niaosong Distict, Kaohsiung, 83301, Taiwan. AD - Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, No.123, Dapi Rd, Niaosong Distict, Kaohsiung, 83301, Taiwan. FAU - Guo, Mindy Ming-Huey AU - Guo MM AD - Kawasaki Disease Center, Kaohsiung Chang Gung Memorial Hospital, No.123, Dapi Rd, Niaosong Distict, Kaohsiung, 83301, Taiwan. AD - Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, No.123, Dapi Rd, Niaosong Distict, Kaohsiung, 83301, Taiwan. FAU - Yu, Hong-Ren AU - Yu HR AD - Kawasaki Disease Center, Kaohsiung Chang Gung Memorial Hospital, No.123, Dapi Rd, Niaosong Distict, Kaohsiung, 83301, Taiwan. AD - Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, No.123, Dapi Rd, Niaosong Distict, Kaohsiung, 83301, Taiwan. FAU - Huang, Fu-Chen AU - Huang FC AD - Kawasaki Disease Center, Kaohsiung Chang Gung Memorial Hospital, No.123, Dapi Rd, Niaosong Distict, Kaohsiung, 83301, Taiwan. AD - Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, No.123, Dapi Rd, Niaosong Distict, Kaohsiung, 83301, Taiwan. FAU - Kuo, Hsing-Chun AU - Kuo HC AD - Department of Nursing, Chang Gung University of Science and Technology, Chiayi, Taiwan. FAU - Kuo, Ho-Chang AU - Kuo HC AD - Kawasaki Disease Center, Kaohsiung Chang Gung Memorial Hospital, No.123, Dapi Rd, Niaosong Distict, Kaohsiung, 83301, Taiwan. erickuo48@yahoo.com.tw. AD - Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, No.123, Dapi Rd, Niaosong Distict, Kaohsiung, 83301, Taiwan. erickuo48@yahoo.com.tw. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160812 PL - England TA - BMC Med Genomics JT - BMC medical genomics JID - 101319628 RN - 0 (Genetic Markers) RN - 0 (Immunoglobulins, Intravenous) SB - IM MH - Case-Control Studies MH - CpG Islands/*genetics MH - DNA Methylation/*immunology MH - Genetic Markers/*genetics MH - Humans MH - Immunoglobulins, Intravenous/*therapeutic use MH - Inflammation/genetics MH - Mucocutaneous Lymph Node Syndrome/*genetics/immunology/*therapy MH - Transcriptome/immunology PMC - PMC4989893 OTO - NOTNLM OT - CpG marker OT - DNA methylation OT - Intravenous immunoglobulin OT - Kawasaki disease OT - Methylation alteration EDAT- 2016/08/19 06:00 MHDA- 2017/10/27 06:00 PMCR- 2016/08/12 CRDT- 2016/08/19 06:00 PHST- 2016/08/19 06:00 [entrez] PHST- 2016/08/19 06:00 [pubmed] PHST- 2017/10/27 06:00 [medline] PHST- 2016/08/12 00:00 [pmc-release] AID - 10.1186/s12920-016-0197-2 [pii] AID - 197 [pii] AID - 10.1186/s12920-016-0197-2 [doi] PST - epublish SO - BMC Med Genomics. 2016 Aug 12;9 Suppl 1(Suppl 1):37. doi: 10.1186/s12920-016-0197-2.