PMID- 27541002
OWN - NLM
STAT- MEDLINE
DCOM- 20170523
LR  - 20201215
IS  - 1553-7404 (Electronic)
IS  - 1553-7390 (Print)
IS  - 1553-7390 (Linking)
VI  - 12
IP  - 8
DP  - 2016 Aug
TI  - Investigating the Interplay between Sister Chromatid Cohesion and Homolog Pairing 
      in Drosophila Nuclei.
PG  - e1006169
LID - 10.1371/journal.pgen.1006169 [doi]
LID - e1006169
AB  - Following DNA replication, sister chromatids must stay connected for the 
      remainder of the cell cycle in order to ensure accurate segregation in the 
      subsequent cell division. This important function involves an evolutionarily 
      conserved protein complex known as cohesin; any loss of cohesin causes premature 
      sister chromatid separation in mitosis. Here, we examined the role of cohesin in 
      sister chromatid cohesion prior to mitosis, using fluorescence in situ 
      hybridization (FISH) to assay the alignment of sister chromatids in interphase 
      Drosophila cells. Surprisingly, we found that sister chromatid cohesion can be 
      maintained in G2 with little to no cohesin. This capacity to maintain cohesion is 
      widespread in Drosophila, unlike in other systems where a reduced dependence on 
      cohesin for sister chromatid segregation has been observed only at specific 
      chromosomal regions, such as the rDNA locus in budding yeast. Additionally, we 
      show that condensin II antagonizes the alignment of sister chromatids in 
      interphase, supporting a model wherein cohesin and condensin II oppose each 
      other's functions in the alignment of sister chromatids. Finally, because the 
      maternal and paternal homologs are paired in the somatic cells of Drosophila, and 
      because condensin II has been shown to antagonize this pairing, we consider the 
      possibility that condensin II-regulated mechanisms for aligning homologous 
      chromosomes may also contribute to sister chromatid cohesion.
FAU - Senaratne, T Niroshini
AU  - Senaratne TN
AD  - Department of Genetics, Harvard Medical School, Boston, Massachusetts, United 
      States of America.
FAU - Joyce, Eric F
AU  - Joyce EF
AD  - Department of Genetics, Harvard Medical School, Boston, Massachusetts, United 
      States of America.
FAU - Nguyen, Son C
AU  - Nguyen SC
AUID- ORCID: 0000-0003-2187-1027
AD  - Department of Genetics, Harvard Medical School, Boston, Massachusetts, United 
      States of America.
FAU - Wu, C-Ting
AU  - Wu CT
AD  - Department of Genetics, Harvard Medical School, Boston, Massachusetts, United 
      States of America.
LA  - eng
GR  - F32 CA157188/CA/NCI NIH HHS/United States
GR  - DP1 GM106412/GM/NIGMS NIH HHS/United States
GR  - RM1 HG008525/HG/NHGRI NIH HHS/United States
GR  - T32 GM096911/GM/NIGMS NIH HHS/United States
GR  - R01 GM085169/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20160819
PL  - United States
TA  - PLoS Genet
JT  - PLoS genetics
JID - 101239074
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Multiprotein Complexes)
RN  - 0 (condensin complexes)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
SB  - IM
MH  - Adenosine Triphosphatases/*genetics
MH  - Animals
MH  - Cell Nucleus/*genetics
MH  - Chromatids/genetics
MH  - Chromosome Segregation/genetics
MH  - Chromosomes/*genetics
MH  - DNA Replication/*genetics
MH  - DNA-Binding Proteins/*genetics
MH  - Drosophila melanogaster/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Metaphase/genetics
MH  - Mitosis/genetics
MH  - Multiprotein Complexes/*genetics
MH  - RNA Interference
MH  - Sister Chromatid Exchange/genetics
PMC - PMC4991795
COIS- The authors have declared that no competing interests exist.
EDAT- 2016/08/20 06:00
MHDA- 2017/05/24 06:00
PMCR- 2016/08/19
CRDT- 2016/08/20 06:00
PHST- 2016/01/28 00:00 [received]
PHST- 2016/06/14 00:00 [accepted]
PHST- 2016/08/20 06:00 [entrez]
PHST- 2016/08/20 06:00 [pubmed]
PHST- 2017/05/24 06:00 [medline]
PHST- 2016/08/19 00:00 [pmc-release]
AID - PGENETICS-D-16-00216 [pii]
AID - 10.1371/journal.pgen.1006169 [doi]
PST - epublish
SO  - PLoS Genet. 2016 Aug 19;12(8):e1006169. doi: 10.1371/journal.pgen.1006169. 
      eCollection 2016 Aug.