PMID- 27542262
OWN - NLM
STAT- MEDLINE
DCOM- 20180222
LR  - 20220321
IS  - 1949-2553 (Electronic)
IS  - 1949-2553 (Linking)
VI  - 7
IP  - 36
DP  - 2016 Sep 6
TI  - Elevated eukaryotic elongation factor 2 expression is involved in proliferation 
      and invasion of lung squamous cell carcinoma.
PG  - 58470-58482
LID - 10.18632/oncotarget.11298 [doi]
AB  - Eukaryotic elongation factor 2 (EF2), is a critical enzyme solely responsible for 
      catalyzing the translocation of the elongated peptidyl-tRNA from the A to P sites 
      of the ribosome during the process of protein synthesis. EF2 is found to be 
      highly expressed in a variety of malignant tumors and is correlated with cancer 
      cell progression and recurrence. The present study was designed to uncover the 
      function of EF2 on lung squamous cell carcinoma (LSCC) cancer cell growth and 
      progression. Our results from clinical tissue studies showed that EF2 protein was 
      significantly overexpressed in LSCC tissues, compared with the adjacent normal 
      lung tissues, which was confirmed by western blotting and tissue microarray. 
      Forced expression of EF2 resulted in the enhancement of lung squamous carcinoma 
      NCI-H520 cells growth through promotion of G2/M progression in cell cycle, 
      activating Akt and Cdc2/Cyclin B1. In nude mice cancer xenograft model, 
      overexpression of EF2 significantly facilitated cell proliferation in vivo. 
      Furthermore, forced expression of EF2 in the cells increased the capabilities of 
      migration and invasion by changing the expressions of EMT-related proteins and 
      genes. These results provided novel insights into the role of EF2 in 
      tumorigenesis and progression in LSCC. EF2-targeted therapy could become a good 
      strategy for the clinical treatment of LSCC.
FAU - Song, Yang
AU  - Song Y
AD  - Department of Histology and Embryology, Dalian Medical University, Dalian, China.
FAU - Sun, Bing
AU  - Sun B
AD  - Department of Chest Surgery, The First Affiliated Hospital of Dalian Medical 
      University, Dalian, China.
FAU - Hao, LiHong
AU  - Hao L
AD  - Department of Histology and Embryology, Dalian Medical University, Dalian, China.
FAU - Hu, Jun
AU  - Hu J
AD  - Department of Histology and Embryology, Dalian Medical University, Dalian, China.
FAU - Du, Sha
AU  - Du S
AD  - Institute of Cancer Stem Cell, Dalian Medical University Cancer Center, Liaoning, 
      China.
FAU - Zhou, Xin
AU  - Zhou X
AD  - Department of Histology and Embryology, Dalian Medical University, Dalian, China.
FAU - Zhang, LiYuan
AU  - Zhang L
AD  - Department of Histology and Embryology, Dalian Medical University, Dalian, China.
FAU - Liu, Lu
AU  - Liu L
AD  - Department of Histology and Embryology, Dalian Medical University, Dalian, China.
FAU - Gong, LinLin
AU  - Gong L
AD  - Department of Histology and Embryology, Dalian Medical University, Dalian, China.
FAU - Chi, XinMing
AU  - Chi X
AD  - Department of Histology and Embryology, Dalian Medical University, Dalian, China.
FAU - Liu, Qiang
AU  - Liu Q
AD  - Institute of Cancer Stem Cell, Dalian Medical University Cancer Center, Liaoning, 
      China.
FAU - Shao, ShuJuan
AU  - Shao S
AD  - Department of Histology and Embryology, Dalian Medical University, Dalian, China.
AD  - Liaoning Key Laboratory of Proteomics, Dalian Medical University, Liaoning, 
      China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Oncotarget
JT  - Oncotarget
JID - 101532965
RN  - 0 (Eukaryotic Initiation Factor-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Carcinoma, Squamous Cell/*metabolism/pathology
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Disease Progression
MH  - Eukaryotic Initiation Factor-2/*metabolism
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Lung Neoplasms/*metabolism/pathology
MH  - Male
MH  - Mice
MH  - Mice, Nude
MH  - Middle Aged
MH  - Neoplasm Invasiveness
MH  - Neoplasm Recurrence, Local/genetics
MH  - Neoplasm Transplantation
MH  - Proteomics/methods
MH  - Tissue Array Analysis
PMC - PMC5295444
OTO - NOTNLM
OT  - eukaryotic elongation factor 2
OT  - invasion
OT  - lung squamous cell carcinoma
OT  - proliferation
OT  - proteomics
COIS- The authors have declared no conflict of interest.
EDAT- 2016/08/20 06:00
MHDA- 2018/02/23 06:00
PMCR- 2016/09/06
CRDT- 2016/08/20 06:00
PHST- 2016/03/16 00:00 [received]
PHST- 2016/07/27 00:00 [accepted]
PHST- 2016/08/20 06:00 [pubmed]
PHST- 2018/02/23 06:00 [medline]
PHST- 2016/08/20 06:00 [entrez]
PHST- 2016/09/06 00:00 [pmc-release]
AID - 11298 [pii]
AID - 10.18632/oncotarget.11298 [doi]
PST - ppublish
SO  - Oncotarget. 2016 Sep 6;7(36):58470-58482. doi: 10.18632/oncotarget.11298.