PMID- 27542617
OWN - NLM
STAT- MEDLINE
DCOM- 20170302
LR  - 20170922
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 57
DP  - 2016 Oct
TI  - Amyloodinum ocellatum in Dicentrarchus labrax: Study of infection in salt water 
      and freshwater aquaponics.
PG  - 179-185
LID - S1050-4648(16)30471-5 [pii]
LID - 10.1016/j.fsi.2016.07.036 [doi]
AB  - This investigation is aimed to improve the knowledge on the physiological 
      alterations occurring at morphological and molecular level in European sea bass 
      naturally infected by A. ocellatum and reared at different salinities. European 
      sea bass juveniles (Dicentrarchus labrax) weighing 20 +/- 0.5 g were divided in 
      three aquaponics systems: CTRL, reared at 20 ppt salinity; AFI, reared in 
      freshwater (0 ppt) and infected with the dinoflagellate Amyloodinium ocellatum; 
      ASI, reared at 20 ppt salinity and infected with A. ocellatum. Beta vulgaris 
      plants were introduced in each of the aquaponic systems. Temperature was 
      increased 1  degrees C every second day from 18 to 25  degrees C during the experiment. At the 
      end of the trial, liver, brain, intestine and gills were sampled for molecular 
      and histological analyses. A. ocellatum affected D. labrax growth (insulin-like 
      growth factor I, IGF-I) and appetite (Neuropeptide Y, NPY) signals in ASI. Immune 
      system was activated in ASI by the presence of parasites by producing higher 
      levels of Interleukin-1 (IL-1) and Tumor Necrosis Factor alpha (TNFalpha). Peroxisome 
      proliferator-activated receptor alpha (PPAR alpha), codifying for a protein involved in 
      lipid metabolism, was upregulated in ASI because of the necessity to produce 
      energy to maintain homeostasis. On the contrary, A. ocellatum did not cause signs 
      of infection in AFI as confirmed by gene expression and histological analysis, 
      that were similar to CTRL. However, in freshwater reared fish, a modification of 
      lipid metabolism was observed through a reduction in PPARalpha gene expression and 
      hepatic lipid content.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Nozzi, Valentina
AU  - Nozzi V
AD  - Department of Life and Environmental Sciences, Universita Politecnica delle 
      Marche, via Brecce Bianche, 60131, Ancona, Italy.
FAU - Strofaldi, Silvia
AU  - Strofaldi S
AD  - Department of Life and Environmental Sciences, Universita Politecnica delle 
      Marche, via Brecce Bianche, 60131, Ancona, Italy.
FAU - Piquer, Isabel Forner
AU  - Piquer IF
AD  - Department of Life and Environmental Sciences, Universita Politecnica delle 
      Marche, via Brecce Bianche, 60131, Ancona, Italy.
FAU - Di Crescenzo, Davide
AU  - Di Crescenzo D
AD  - Aquaguide s.a.s. Di Davide Di Crescenzo & Co., Via IV novembre 8, 60015, 
      Falconara Marittima, AN, Italy.
FAU - Olivotto, Ike
AU  - Olivotto I
AD  - Department of Life and Environmental Sciences, Universita Politecnica delle 
      Marche, via Brecce Bianche, 60131, Ancona, Italy.
FAU - Carnevali, Oliana
AU  - Carnevali O
AD  - Department of Life and Environmental Sciences, Universita Politecnica delle 
      Marche, via Brecce Bianche, 60131, Ancona, Italy. Electronic address: 
      o.carnevali@univpm.it.
LA  - eng
PT  - Journal Article
DEP - 20160816
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
SB  - IM
MH  - Animals
MH  - *Bass
MH  - Dinoflagellida/*physiology
MH  - Fish Diseases/genetics/immunology/parasitology/*physiopathology
MH  - Fresh Water/*analysis
MH  - Gene Expression
MH  - Protozoan Infections, Animal/genetics/immunology/parasitology/*physiopathology
MH  - Salinity
MH  - Seawater/*analysis
OTO - NOTNLM
OT  - A. ocellatum
OT  - Aquaponics
OT  - D. labrax
OT  - Disease
OT  - Gills
OT  - Hepatic lipid
OT  - IGF
OT  - NPY
OT  - PPARalpha
EDAT- 2016/08/21 06:00
MHDA- 2017/03/03 06:00
CRDT- 2016/08/21 06:00
PHST- 2016/03/29 00:00 [received]
PHST- 2016/07/12 00:00 [revised]
PHST- 2016/07/31 00:00 [accepted]
PHST- 2016/08/21 06:00 [entrez]
PHST- 2016/08/21 06:00 [pubmed]
PHST- 2017/03/03 06:00 [medline]
AID - S1050-4648(16)30471-5 [pii]
AID - 10.1016/j.fsi.2016.07.036 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2016 Oct;57:179-185. doi: 10.1016/j.fsi.2016.07.036. Epub 
      2016 Aug 16.