PMID- 27542821
OWN - NLM
STAT- MEDLINE
DCOM- 20171018
LR  - 20191210
IS  - 1532-2750 (Electronic)
IS  - 1098-612X (Linking)
VI  - 19
IP  - 8
DP  - 2017 Aug
TI  - Determination of reference intervals and comparison of venous blood gas 
      parameters using standard and non-standard collection methods in 24 cats.
PG  - 831-840
LID - 10.1177/1098612X16663269 [doi]
AB  - Objectives The aim of this study was to determine in-house reference intervals 
      (RIs) for venous blood analysis with the RAPIDPoint 500 blood gas analyser using 
      blood gas syringes (BGSs) and to determine whether immediate analysis of venous 
      blood collected into lithium heparin (LH) tubes can replace anaerobic blood 
      sampling into BGSs. Methods Venous blood was collected from 24 healthy cats and 
      directly transferred into a BGS and an LH tube. The BGS was immediately analysed 
      on the RAPIDPoint 500 followed by the LH tube. The BGSs and LH tubes were 
      compared using paired t-test or Wilcoxon matched-pairs signed-rank test, 
      Bland-Altman and Passing-Bablok analysis. To assess clinical relevance, bias or 
      percentage bias between BGSs and LH tubes was compared with the allowable total 
      error (TEa) recommended for the respective parameter. Results Based on the values 
      obtained from the BGSs, RIs were calculated for the evaluated parameters, 
      including blood gases, electrolytes, glucose and lactate. Values derived from LH 
      tubes showed no significant difference for standard bicarbonate, whole blood base 
      excess, haematocrit, total haemoglobin, sodium, potassium, chloride, glucose and 
      lactate, while pH, partial pressure of carbon dioxide and oxygen, actual 
      bicarbonate, extracellular base excess, ionised calcium and anion gap were 
      significantly different to the samples collected in BGSs ( P <0.05). Furthermore, 
      pH, partial pressure of carbon dioxide and oxygen, extracellular base excess, 
      ionised calcium and anion gap exceeded the recommended TEa. Conclusions and 
      relevance Assessment of actual and standard bicarbonate, whole blood base excess, 
      haematocrit, total haemoglobin, sodium, potassium, chloride, glucose and lactate 
      can be made based on blood collected in LH tubes and analysed within 5 mins. For 
      pH, partial pressure of carbon dioxide and oxygen, extracellular base excess, 
      anion gap and ionised calcium the clinically relevant alterations have to be 
      considered if analysed in LH tubes.
FAU - Bachmann, Karin
AU  - Bachmann K
AD  - 1 Department for Small Animals, Vetsuisse Faculty, University of Zurich, Zurich, 
      Switzerland.
FAU - Kutter, Annette Pn
AU  - Kutter AP
AD  - 2 Section of Anaesthesiology, Equine Department, Vetsuisse Faculty, University of 
      Zurich, Zurich, Switzerland.
FAU - Schefer, Rahel Jud
AU  - Schefer RJ
AD  - 1 Department for Small Animals, Vetsuisse Faculty, University of Zurich, Zurich, 
      Switzerland.
FAU - Marly-Voquer, Charlotte
AU  - Marly-Voquer C
AD  - 2 Section of Anaesthesiology, Equine Department, Vetsuisse Faculty, University of 
      Zurich, Zurich, Switzerland.
FAU - Sigrist, Nadja
AU  - Sigrist N
AD  - 1 Department for Small Animals, Vetsuisse Faculty, University of Zurich, Zurich, 
      Switzerland.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20160801
PL  - England
TA  - J Feline Med Surg
JT  - Journal of feline medicine and surgery
JID - 100897329
RN  - 0 (Blood Glucose)
RN  - 142M471B3J (Carbon Dioxide)
RN  - 33X04XA5AT (Lactic Acid)
RN  - RWP5GA015D (Potassium)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Blood Gas Analysis/instrumentation/*veterinary
MH  - Blood Glucose
MH  - Blood Specimen Collection/*veterinary
MH  - Calcium/blood
MH  - Carbon Dioxide/blood
MH  - Cats/*blood
MH  - Female
MH  - Hydrogen-Ion Concentration
MH  - Lactic Acid/blood
MH  - Male
MH  - Potassium/blood
MH  - Reference Values
EDAT- 2016/08/21 06:00
MHDA- 2017/10/19 06:00
CRDT- 2016/08/21 06:00
PHST- 2016/08/21 06:00 [pubmed]
PHST- 2017/10/19 06:00 [medline]
PHST- 2016/08/21 06:00 [entrez]
AID - 1098612X16663269 [pii]
AID - 10.1177/1098612X16663269 [doi]
PST - ppublish
SO  - J Feline Med Surg. 2017 Aug;19(8):831-840. doi: 10.1177/1098612X16663269. Epub 
      2016 Aug 1.