PMID- 27542839
OWN - NLM
STAT- MEDLINE
DCOM- 20171128
LR  - 20181113
IS  - 1471-2474 (Electronic)
IS  - 1471-2474 (Linking)
VI  - 17
DP  - 2016 Aug 19
TI  - The effects of dopamine receptor 2 expression on B cells on bone metabolism and 
      TNF-alpha levels in rheumatoid arthritis.
PG  - 352
LID - 10.1186/s12891-016-1220-7 [doi]
LID - 352
AB  - BACKGROUND: Dopamine receptor 2 (DR2) expressions on B cells from Rheumatoid 
      arthritis (RA) patients has been found to be negatively correlated with disease 
      activity and can potentially predict the response to treatment. This study aimed 
      to investigate the role of B cell DR2 expression on bone remodeling in RA. 
      METHODS: Patients with RA (n = 14) or osteoarthritis (OA; n = 12), and healthy 
      controls (n = 12) were recruited for this study. Dopamine receptor (DR) 2 
      expression was assessed using flow cytometry. Pro-inflammatory cytokines, 
      including interleuin(IL)-1beta, IL-6, IL-17, and tumor necrosis factor(TNF)-alpha, and 
      bone turnovers, including osteocalcin (OC),serum procollagen type I N propeptide 
      (PINP), C-terminal telopeptide of type I collagen (beta-CTX), collagen type I 
      cross-linked telopeptide (ICTP), as well as matrix metalloproteinase-3 (MMP-3) 
      and osteoprotegerin (OPG) were measured by electrochemiluminescence, 
      chemiluminescence, or enzyme-linked immunosorbent assay. DR2 expression on 
      synovial B cells from 4 RA patients and 3 OA patients was detected by 
      immunofluorescence. RESULTS: There were more DR2(+)CD19(+) B cells in synovial 
      tissues from RA patients than in those from OA patients. The frequency of 
      peripheral B cells that expressed DR2 was positively correlated with plasma TNF-alpha 
      level. Levels of ICTP and MMP-3 were significantly higher, and OPG were lower in 
      RA patients compared to those in the OA group and healthy controls (all 
      P < 0.05). CONCLUSION: The frequency of B cells that expressed DR2 showed a 
      correlation with levels of the pro-inflammatory cytokine TNF-alpha. DR2(+)CD19(+) B 
      cells in synovial tissues might have a role in bone metabolism and TNF-alpha 
      production.
FAU - Wei, Lei
AU  - Wei L
AD  - Department of Rheumatology, Zhongshan Hospital, Fudan University, No. 180, Road 
      Fenglin, Shanghai, 200032, People's Republic of China.
FAU - Sun, Ying
AU  - Sun Y
AD  - Department of Rheumatology, Zhongshan Hospital, Fudan University, No. 180, Road 
      Fenglin, Shanghai, 200032, People's Republic of China.
FAU - Kong, Xiu-Fang
AU  - Kong XF
AD  - Department of Rheumatology, Zhongshan Hospital, Fudan University, No. 180, Road 
      Fenglin, Shanghai, 200032, People's Republic of China.
FAU - Zhang, Chi
AU  - Zhang C
AD  - Department of Orthopedics, Zhongshan Hospital, Fudan University, Shanghai, China.
FAU - Yue, Tao
AU  - Yue T
AD  - Department of Rheumatology, Guanghua Integrative Medicine Hospital, Shanghai, 
      China.
FAU - Zhu, Qi
AU  - Zhu Q
AD  - Department of Rheumatology, Guanghua Integrative Medicine Hospital, Shanghai, 
      China.
FAU - He, Dong-Yi
AU  - He DY
AD  - Department of Rheumatology, Guanghua Integrative Medicine Hospital, Shanghai, 
      China.
FAU - Jiang, Lin-Di
AU  - Jiang LD
AD  - Department of Rheumatology, Zhongshan Hospital, Fudan University, No. 180, Road 
      Fenglin, Shanghai, 200032, People's Republic of China. zsh-rheum@hotmail.com.
AD  - Center of evidence based medicine, Fudan University, Shanghai, China. 
      zsh-rheum@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160819
PL  - England
TA  - BMC Musculoskelet Disord
JT  - BMC musculoskeletal disorders
JID - 100968565
RN  - 0 (Antirheumatic Agents)
RN  - 0 (Cytokines)
RN  - 0 (Receptors, Dopamine D2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antirheumatic Agents/therapeutic use
MH  - Arthritis, Rheumatoid/drug therapy/immunology/*metabolism
MH  - B-Lymphocytes/*metabolism
MH  - *Bone Remodeling
MH  - Case-Control Studies
MH  - Cytokines/blood
MH  - Female
MH  - Humans
MH  - Joint Capsule/metabolism
MH  - Male
MH  - Middle Aged
MH  - Osteoarthritis/metabolism
MH  - Receptors, Dopamine D2/*metabolism
MH  - Young Adult
PMC - PMC4992283
OTO - NOTNLM
OT  - B cells
OT  - Bone metabolism
OT  - Dopamine receptor
OT  - Rheumatoid arthritis
EDAT- 2016/08/21 06:00
MHDA- 2017/11/29 06:00
PMCR- 2016/08/19
CRDT- 2016/08/21 06:00
PHST- 2015/12/17 00:00 [received]
PHST- 2016/08/13 00:00 [accepted]
PHST- 2016/08/21 06:00 [entrez]
PHST- 2016/08/21 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2016/08/19 00:00 [pmc-release]
AID - 10.1186/s12891-016-1220-7 [pii]
AID - 1220 [pii]
AID - 10.1186/s12891-016-1220-7 [doi]
PST - epublish
SO  - BMC Musculoskelet Disord. 2016 Aug 19;17:352. doi: 10.1186/s12891-016-1220-7.