PMID- 27542932
OWN - NLM
STAT- MEDLINE
DCOM- 20171027
LR  - 20181113
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 82
IP  - 21
DP  - 2016 Nov 1
TI  - Simultaneous Transformation of Commingled Trichloroethylene, Tetrachloroethylene, 
      and 1,4-Dioxane by a Microbially Driven Fenton Reaction in Batch Liquid Cultures.
PG  - 6335-6343
AB  - Improper disposal of 1,4-dioxane and the chlorinated organic solvents 
      trichloroethylene (TCE) and tetrachloroethylene (also known as perchloroethylene 
      [PCE]) has resulted in widespread contamination of soil and groundwater. In the 
      present study, a previously designed microbially driven Fenton reaction system 
      was reconfigured to generate hydroxyl (HO ) radicals for simultaneous 
      transformation of source zone levels of single, binary, and ternary mixtures of 
      TCE, PCE, and 1,4-dioxane. The reconfigured Fenton reaction system was driven by 
      fed batch cultures of the Fe(III)-reducing facultative anaerobe Shewanella 
      oneidensis amended with lactate, Fe(III), and contaminants and exposed to 
      alternating anaerobic and aerobic conditions. To avoid contaminant loss due to 
      volatility, the Fe(II)-generating, hydrogen peroxide-generating, and contaminant 
      transformation phases of the microbially driven Fenton reaction system were 
      separated. The reconfigured Fenton reaction system transformed TCE, PCE, and 
      1,4-dioxane either as single contaminants or as binary and ternary mixtures. In 
      the presence of equimolar concentrations of PCE and TCE, the ratio of the 
      experimentally derived rates of PCE and TCE transformation was nearly identical 
      to the ratio of the corresponding HO  radical reaction rate constants. The 
      reconfigured Fenton reaction system may be applied as an ex situ platform for 
      simultaneous degradation of commingled TCE, PCE, and 1,4-dioxane and provides 
      valuable information for future development of in situ remediation technologies. 
      IMPORTANCE: A microbially driven Fenton reaction system [driven by the 
      Fe(III)-reducing facultative anaerobe S. oneidensis] was reconfigured to 
      transform source zone levels of TCE, PCE, and 1,4-dioxane as single contaminants 
      or as binary and ternary mixtures. The microbially driven Fenton reaction may 
      thus be applied as an ex situ platform for simultaneous degradation of at least 
      three (and potentially more) commingled contaminants. Additional targets for ex 
      situ and in situ degradation by the microbially driven Fenton reaction developed 
      in the present study include multiple combinations of environmental contaminants 
      susceptible to attack by Fenton reaction-generated HO  radicals, including 
      commingled plumes of 1,4-dioxane, pentachlorophenol (PCP), PCE, TCE, 
      1,1,2-trichloroethane (TCA), and perfluoroalkylated substances (PFAS).
CI  - Copyright (c) 2016, American Society for Microbiology. All Rights Reserved.
FAU - Sekar, Ramanan
AU  - Sekar R
AD  - School of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA.
FAU - Taillefert, Martial
AU  - Taillefert M
AD  - School of Earth and Atmospheric Sciences, Georgia Institute of Technology, 
      Atlanta, Georgia, USA.
FAU - DiChristina, Thomas J
AU  - DiChristina TJ
AD  - School of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA 
      thomas.dichristina@biology.gatech.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20161014
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Dioxanes)
RN  - 0 (Fenton's reagent)
RN  - 0 (Water Pollutants, Chemical)
RN  - 290YE8AR51 (Trichloroethylene)
RN  - 3352-57-6 (Hydroxyl Radical)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - E1UOL152H7 (Iron)
RN  - J8A3S10O7S (1,4-dioxane)
RN  - TJ904HH8SN (Tetrachloroethylene)
SB  - IM
MH  - Aerobiosis
MH  - Anaerobiosis
MH  - Batch Cell Culture Techniques
MH  - *Biodegradation, Environmental
MH  - Dioxanes/*metabolism
MH  - Environmental Restoration and Remediation
MH  - Hydrogen Peroxide
MH  - Hydroxyl Radical
MH  - Iron
MH  - Shewanella/growth & development/*metabolism
MH  - Tetrachloroethylene/*metabolism
MH  - Trichloroethylene/*metabolism
MH  - Water Pollutants, Chemical/metabolism
PMC - PMC5066350
EDAT- 2016/08/21 06:00
MHDA- 2017/10/28 06:00
PMCR- 2017/04/14
CRDT- 2016/08/21 06:00
PHST- 2016/08/07 00:00 [received]
PHST- 2016/08/10 00:00 [accepted]
PHST- 2016/08/21 06:00 [pubmed]
PHST- 2017/10/28 06:00 [medline]
PHST- 2016/08/21 06:00 [entrez]
PHST- 2017/04/14 00:00 [pmc-release]
AID - AEM.02325-16 [pii]
AID - 02325-16 [pii]
AID - 10.1128/AEM.02325-16 [doi]
PST - epublish
SO  - Appl Environ Microbiol. 2016 Oct 14;82(21):6335-6343. doi: 10.1128/AEM.02325-16. 
      Print 2016 Nov 1.