PMID- 27543614
OWN - NLM
STAT- MEDLINE
DCOM- 20170808
LR  - 20180122
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 197
IP  - 6
DP  - 2016 Sep 15
TI  - Diverse HLA-I Peptide Repertoires of the APC Lines MUTZ3-Derived Immature and 
      Mature Dendritic Cells and THP1-Derived Macrophages.
PG  - 2102-9
LID - 10.4049/jimmunol.1600762 [doi]
AB  - Dendritic cells (DCs) and macrophages are specialized APCs that process and 
      present self-Ags for induction of tolerance and foreign Ags to initiate T 
      cell-mediated immunity. Related to differentiation states they have specific 
      phenotypes and functions. However, the impact of these differentiations on Ag 
      processing and presentation remains poorly defined. To gain insight into this, we 
      analyzed and compared the HLA-I peptidomes of MUTZ3-derived human immature and 
      mature DC lines and THP1-derived macrophages by liquid chromatography tandem mass 
      spectrometry. We found that the HLA-I peptidomes were heterogeneous and 
      individualized and were dominated by nonapeptides with similar HLA-I binding 
      affinities and anchor residues. MUTZ3-derived DCs and THP1-derived macrophages 
      were able to sample peptides from source proteins of almost all subcellular 
      locations and were involved in various cellular functions in similar proportion, 
      with preference to proteins involved in cell communication, signal transduction, 
      protein metabolism, and transcription factor/regulator activity.
CI  - Copyright (c) 2016 by The American Association of Immunologists, Inc.
FAU - Nyambura, Lydon Wainaina
AU  - Nyambura LW
AUID- ORCID: 0000-0003-2197-6048
AD  - Klinische Forschergruppe Tumorimmunologie, Klinik fur Dermatologie, Venerologie 
      and Allergologie, Charite-Universitatsmedizin Berlin, 10098 Berlin, Germany; and 
      Humboldt Universitat zu Berlin, Institut fur Biologie, Lebenswissenschaftliche 
      Fakultat, 10115 Berlin, Germany.
FAU - Jarmalavicius, Saulius
AU  - Jarmalavicius S
AD  - Klinische Forschergruppe Tumorimmunologie, Klinik fur Dermatologie, Venerologie 
      and Allergologie, Charite-Universitatsmedizin Berlin, 10098 Berlin, Germany; and.
FAU - Baleeiro, Renato Brito
AU  - Baleeiro RB
AUID- ORCID: 0000-0001-7568-7570
AD  - Klinische Forschergruppe Tumorimmunologie, Klinik fur Dermatologie, Venerologie 
      and Allergologie, Charite-Universitatsmedizin Berlin, 10098 Berlin, Germany; and.
FAU - Walden, Peter
AU  - Walden P
AUID- ORCID: 0000-0002-8986-3667
AD  - Klinische Forschergruppe Tumorimmunologie, Klinik fur Dermatologie, Venerologie 
      and Allergologie, Charite-Universitatsmedizin Berlin, 10098 Berlin, Germany; and 
      peter.walden@charite.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160819
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Peptides)
SB  - IM
MH  - Antigen-Presenting Cells/metabolism
MH  - Binding Sites
MH  - Cell Line, Tumor
MH  - Dendritic Cells/*metabolism
MH  - Histocompatibility Antigens Class I/*metabolism
MH  - Humans
MH  - Macrophages/*metabolism
MH  - Peptides/*metabolism
EDAT- 2016/08/21 06:00
MHDA- 2017/08/09 06:00
CRDT- 2016/08/21 06:00
PHST- 2016/04/29 00:00 [received]
PHST- 2016/07/15 00:00 [accepted]
PHST- 2016/08/21 06:00 [entrez]
PHST- 2016/08/21 06:00 [pubmed]
PHST- 2017/08/09 06:00 [medline]
AID - jimmunol.1600762 [pii]
AID - 10.4049/jimmunol.1600762 [doi]
PST - ppublish
SO  - J Immunol. 2016 Sep 15;197(6):2102-9. doi: 10.4049/jimmunol.1600762. Epub 2016 
      Aug 19.