PMID- 27543737
OWN - NLM
STAT- MEDLINE
DCOM- 20190628
LR  - 20211204
IS  - 0125-877X (Print)
IS  - 0125-877X (Linking)
VI  - 35
IP  - 2
DP  - 2017 Jun
TI  - Frequency of HLA-DQB1*0201/02 and DQB1*0302 alleles and tissue transglutaminase 
      antibody seropositivity in children with type 1 diabetes mellitus.
PG  - 82-85
LID - 10.12932/AP0751 [doi]
AB  - BACKGROUND: Patients with type 1 diabetes mellitus (T1DM) have an increased risk 
      of celiac disease (CD). Both diseases have a common genetic susceptibility locus 
      in the human leukocyte antigen (HLA) class II alleles. Testing for tissue 
      transglutaminase antibodies (anti-tTG) is highly accurate for a CD diagnosis. 
      OBJECTIVE: To determine the frequency of HLA-DQB1*0201/02 and DQB1*0302 alleles 
      and anti-tTG seropositivity in children with T1DM. METHOD: Forty-six children 
      with T1DM (male:female=24:22; mean age 12+/-3.7 years) without significant 
      digestive symptoms were enrolled. The mean duration of diabetes was 5+/-3.5 years. 
      Serum anti-tTG IgA and IgG as well as HLA-DQ2 (DQB1*0201/02) and -DQ8 (DQB1*0302) 
      alleles were analyzed. The allele frequencies were compared with those in 
      controls, which included 124 normal Thai individuals, as reported in our previous 
      study. RESULTS: All subjects were negative for anti-tTG IgG. Only one patient 
      (2.2%) was positive for anti-tTG IgA (38.5 U/mL; cut-off 15 U/mL). Although this 
      patient was also heterozygous for HLA-DQ2 and was asymptomatic for CD, he 
      declined endoscopic confirmation. Twenty-nine of 46 patients carried HLA-DQ2 
      and/or -DQ8 heterodimers. HLA-DQB1*0201/02 and HLA-DQB1*0302 allele frequencies 
      were significantly higher (27% and 14%) in T1DM patients compared with normal 
      controls (13.3% and 7.3%; P<0.001 and P=0.002, respectively). CONCLUSIONS: A 
      significantly greater frequency of DQB1*0201/02 and DQB1*0302 alleles were 
      present in children with T1DM compared with the control group. This indicates a 
      potentially important role of these alleles in the development of T1DM. The 
      prevalence of CD screening by serologic testing is negligible in Thai children 
      with T1DM.
FAU - Thammarakcharoen, Thanita
AU  - Thammarakcharoen T
AD  - Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, 
      Thailand.
FAU - Hirankarn, Nattiya
AU  - Hirankarn N
AD  - Center of Excellence in Immunology and Immune-mediated Diseases, Department of 
      Microbiology, Faculty of Medicine, Chulalongkorn University.
FAU - Sahakitrungruang, Taninee
AU  - Sahakitrungruang T
AD  - Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, 
      Thailand.
FAU - Thongmee, Thanunrat
AU  - Thongmee T
AD  - Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, 
      Thailand.
FAU - Kuptawintu, Pavinee
AU  - Kuptawintu P
AD  - National Blood Center, The Thai Red Cross Society.
FAU - Kanoonthong, Sriprapai
AU  - Kanoonthong S
AD  - National Blood Center, The Thai Red Cross Society.
FAU - Chongsrisawat, Voranush
AU  - Chongsrisawat V
AD  - Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, 
      Thailand.
LA  - eng
GR  - T32 DK007161/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PL  - Thailand
TA  - Asian Pac J Allergy Immunol
JT  - Asian Pacific journal of allergy and immunology
JID - 8402034
RN  - 0 (Autoantibodies)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ beta-Chains)
RN  - 0 (HLA-DQ2 antigen)
RN  - 0 (HLA-DQB1 antigen)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - EC 2.3.2.13 (Protein Glutamine gamma Glutamyltransferase 2)
RN  - EC 2.3.2.13 (Transglutaminases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Adolescent
MH  - Alleles
MH  - Autoantibodies/*blood
MH  - Celiac Disease/immunology
MH  - Child
MH  - Child, Preschool
MH  - Diabetes Mellitus, Type 1/*genetics/*immunology
MH  - Female
MH  - GTP-Binding Proteins/*immunology
MH  - Gene Frequency
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - HLA-DQ Antigens/genetics
MH  - HLA-DQ beta-Chains/*genetics
MH  - Haplotypes
MH  - Histocompatibility Testing
MH  - Humans
MH  - Immunoglobulin A/blood
MH  - Immunoglobulin G/blood
MH  - Male
MH  - Protein Glutamine gamma Glutamyltransferase 2
MH  - Transglutaminases/*immunology
EDAT- 2016/08/21 06:00
MHDA- 2019/06/30 06:00
CRDT- 2016/08/21 06:00
PHST- 2016/08/21 06:00 [pubmed]
PHST- 2019/06/30 06:00 [medline]
PHST- 2016/08/21 06:00 [entrez]
AID - 10.12932/AP0751 [doi]
PST - ppublish
SO  - Asian Pac J Allergy Immunol. 2017 Jun;35(2):82-85. doi: 10.12932/AP0751.