PMID- 27544306
OWN - NLM
STAT- MEDLINE
DCOM- 20170626
LR  - 20180814
IS  - 1090-2163 (Electronic)
IS  - 0008-8749 (Linking)
VI  - 310
DP  - 2016 Dec
TI  - Role of Wnt3a expressed by dendritic cells in the activation of canonical Wnt 
      signaling and generation of memory T cells during primary immune responses.
PG  - 99-107
LID - S0008-8749(16)30077-6 [pii]
LID - 10.1016/j.cellimm.2016.08.005 [doi]
AB  - The presence of memory T cells (T(M)s) hinders transplant survival. Dendritic 
      cells (DCs) induce the generation of T(M)s during primary immune responses. 
      However, the specific mechanisms are unclear. In this study, we constructed a 
      Wnt3a-expressing adenovirus and used small interfering RNA (siRNA) targeting 
      Wnt3a to investigate the influence of Wnt3a expression in DCs on the generation 
      of T(M)s during primary immune responses. Our results demonstrated that the Wnt3a 
      expression levels in DCs influenced the generation of T(M)s after 5days in 
      co-culture with naive T cells through activation of the Wnt canonical pathway. 
      Interleukin-7 secretion levels in supernatants of DC/T(N)s co-cultures showed a 
      similar pattern of Wnt3a expression levels in DCs. These findings provide a 
      better understanding of T(M)s generation mechanisms that might be useful to 
      improve transplant outcomes.
CI  - Copyright A(c) 2016 Elsevier Inc. All rights reserved.
FAU - Luo, Lei
AU  - Luo L
AD  - Department of Research and Education, Guizhou Province People's Hospital, Guiyang 
      550002, China.
FAU - Li, Zhengyu
AU  - Li Z
AD  - Department of Thoracic Surgery, Guizhou Province People's Hospital, Guiyang 
      550002, China.
FAU - Luo, Guangheng
AU  - Luo G
AD  - Department of Urology, Guizhou Province People's Hospital, Guiyang 550002, China.
FAU - Zhao, Yingting
AU  - Zhao Y
AD  - Department of Research and Education, Guizhou Province People's Hospital, Guiyang 
      550002, China.
FAU - Yang, Jing
AU  - Yang J
AD  - Department of Cardiology, Guizhou Province People's Hospital, Guiyang 550002, 
      China.
FAU - Chen, Hui
AU  - Chen H
AD  - Department of Research and Education, Guizhou Province People's Hospital, Guiyang 
      550002, China. Electronic address: huichen7710@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160813
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Interleukin-7)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Wnt3A Protein)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/*immunology
MH  - Graft Rejection/*immunology/prevention & control
MH  - Immunologic Memory
MH  - Interleukin-7/metabolism
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - RNA, Small Interfering/genetics
MH  - Signal Transduction/immunology
MH  - T-Lymphocytes/*immunology
MH  - Wnt3A Protein/genetics/*metabolism
OTO - NOTNLM
OT  - Adenoviral expression vector
OT  - Canonical Wnt pathway
OT  - Dendritic cells
OT  - Memory T cells
OT  - Small interfering RNA
OT  - Wnt3a
EDAT- 2016/08/22 06:00
MHDA- 2017/06/27 06:00
CRDT- 2016/08/22 06:00
PHST- 2016/06/06 00:00 [received]
PHST- 2016/08/12 00:00 [revised]
PHST- 2016/08/13 00:00 [accepted]
PHST- 2016/08/22 06:00 [pubmed]
PHST- 2017/06/27 06:00 [medline]
PHST- 2016/08/22 06:00 [entrez]
AID - S0008-8749(16)30077-6 [pii]
AID - 10.1016/j.cellimm.2016.08.005 [doi]
PST - ppublish
SO  - Cell Immunol. 2016 Dec;310:99-107. doi: 10.1016/j.cellimm.2016.08.005. Epub 2016 
      Aug 13.