PMID- 27544315
OWN - NLM
STAT- MEDLINE
DCOM- 20170314
LR  - 20191210
IS  - 1423-0291 (Electronic)
IS  - 1015-2008 (Linking)
VI  - 84
IP  - 2
DP  - 2017
TI  - Clinical Usefulness of a One-Tube Nested Reverse Transcription Quantitative 
      Polymerase Chain Reaction Assay for Evaluating Human Epidermal Growth Factor 
      Receptor 2 mRNA Overexpression in Formalin-Fixed and Paraffin-Embedded Breast 
      Cancer Tissue Samples.
PG  - 57-70
LID - 10.1159/000447301 [doi]
AB  - BACKGROUND: Currently, the two main methods used to analyze human epidermal 
      growth factor receptor 2 (HER2) amplification or overexpression have a limited 
      accuracy and high costs. These limitations can be overcome by the development of 
      complementary quantitative methods. METHODS: In this study, we analyzed HER2 mRNA 
      expression in clinical formalin-fixed and paraffin-embedded (FFPE) samples using 
      a one-tube nested reverse transcription quantitative polymerase chain reaction 
      (RT-qPCR) assay. We measured expression relative to 3 reference genes and 
      compared the results to those obtained by conventional immunohistochemistry (IHC) 
      and fluorescence in situ hybridization (FISH) assays with 226 FFPE breast cancer 
      tissue samples. RESULTS: The one-tube nested RT-qPCR assay proved to be highly 
      sensitive and specific based on comparisons with IHC (96.9 and 97.7%, 
      respectively) and FISH (92.4 and 92.9%, respectively) obtained with the 
      validation set. Comparisons with clinicopathological data revealed significant 
      associations between HER2 overexpression and TNM stage (p < 0.01), histological 
      type (p < 0.01), ER status (p < 0.001), PR status (p < 0.05), HER2 status (p < 
      0.001), and molecular subtypes (p < 0.001). CONCLUSION: Based on these findings, 
      our one-tube nested RT-qPCR assay is a potentially useful and complementary 
      screening tool for the detection of HER2 mRNA overexpression.
CI  - (c) 2016 S. Karger AG, Basel.
FAU - Wang, Hye-Young
AU  - Wang HY
AD  - Optipharm M&amp;D, Inc., Wonju Eco Environmental Technology Center, Wonju, 
      Republic of Korea.
FAU - Ahn, Sungwoo
AU  - Ahn S
FAU - Park, Sunyoung
AU  - Park S
FAU - Kim, SeungIl
AU  - Kim S
FAU - Lee, Hyeyoung
AU  - Lee H
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20160820
PL  - Switzerland
TA  - Pathobiology
JT  - Pathobiology : journal of immunopathology, molecular and cellular biology
JID - 9007504
RN  - 0 (Biomarkers, Tumor)
RN  - 1HG84L3525 (Formaldehyde)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Biomarkers, Tumor
MH  - Breast/*metabolism/pathology
MH  - Breast Neoplasms/*genetics/metabolism/pathology
MH  - Cell Line, Tumor
MH  - Female
MH  - Formaldehyde
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Paraffin Embedding
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptor, ErbB-2/*genetics/metabolism
MH  - Sensitivity and Specificity
MH  - Tissue Fixation
EDAT- 2016/08/22 06:00
MHDA- 2017/03/16 06:00
CRDT- 2016/08/22 06:00
PHST- 2016/03/14 00:00 [received]
PHST- 2016/05/31 00:00 [accepted]
PHST- 2016/08/22 06:00 [pubmed]
PHST- 2017/03/16 06:00 [medline]
PHST- 2016/08/22 06:00 [entrez]
AID - 000447301 [pii]
AID - 10.1159/000447301 [doi]
PST - ppublish
SO  - Pathobiology. 2017;84(2):57-70. doi: 10.1159/000447301. Epub 2016 Aug 20.