PMID- 27545763
OWN - NLM
STAT- MEDLINE
DCOM- 20170410
LR  - 20211203
IS  - 1439-0531 (Electronic)
IS  - 0936-6768 (Linking)
VI  - 51
IP  - 5
DP  - 2016 Oct
TI  - Mechanistic target of rapamycin is activated in bovine granulosa cells after LH 
      surge but is not essential for ovulation.
PG  - 766-73
LID - 10.1111/rda.12745 [doi]
AB  - The LH surge induces functional and morphological changes in granulosa cells. 
      Mechanistic target of rapamycin (mTOR) is an integrator of signalling pathways in 
      multiple cell types. We hypothesized that mTOR kinase activity integrates and 
      modulates molecular pathways induced by LH in granulosa cells during the 
      preovulatory period. Cows were ovariectomized and granulosa cells collected at 0, 
      3, 6, 12 and 24 hr after GnRH injection. While RHEB mRNA levels increased at 3 
      and 6 hr, returning to basal levels by 12 hr after GnRH treatment, RHOA mRNA 
      levels increased at 6 hr and remained high thereafter. Western blot analyses 
      revealed increased S6K phosphorylation at 3 and 6 hr after GnRH injection. 
      Similarly, mRNA levels of ERK1/2, STAR and EGR-1 were higher 3 hr after GnRH 
      treatment. Rapamycin treatment inhibited mTOR activity and increased AKT 
      activity, but did not alter ERK1/2 phosphorylation and EGR1 protein levels in 
      cultured bovine granulosa cells. Rapamycin also inhibited LH-induced increase in 
      EREG mRNA abundance in granulosa cells in vitro. However, intrafollicular 
      injection of rapamycin did not suppress ovulation. These findings suggest that 
      mTOR is involved in the control of EREG expression in cattle, which may be 
      triggered by LH surge stimulating RHEB and S6K activity.
CI  - (c) 2016 Blackwell Verlag GmbH.
FAU - da Rosa, Pra
AU  - da Rosa P
AD  - Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University 
      of Santa Maria, Santa Maria, RS, Brazil.
FAU - Dau, Amp
AU  - Dau A
AD  - Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University 
      of Santa Maria, Santa Maria, RS, Brazil.
FAU - De Cesaro, M P
AU  - De Cesaro MP
AD  - Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University 
      of Santa Maria, Santa Maria, RS, Brazil.
FAU - Dos Santos, J T
AU  - Dos Santos JT
AD  - Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University 
      of Santa Maria, Santa Maria, RS, Brazil.
FAU - Gasperin, B G
AU  - Gasperin BG
AD  - Laboratory of Animal Reproduction - ReproPEL, Federal University of Pelotas, 
      Pelotas, RS, Brazil.
FAU - Duggavathi, R
AU  - Duggavathi R
AD  - Department of Animal Science, McGill University, Sainte-Anne-de-Bellevue, QC, 
      Canada.
FAU - Bordignon, V
AU  - Bordignon V
AD  - Department of Animal Science, McGill University, Sainte-Anne-de-Bellevue, QC, 
      Canada. vilceu.bordignon@mcgill.ca.
FAU - Goncalves, Pbd
AU  - Goncalves P
AD  - Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University 
      of Santa Maria, Santa Maria, RS, Brazil. bayard@ufsm.br.
LA  - eng
SI  - GENBANK/NM_001031764.1
SI  - GENBANK/NM_174381.1
SI  - GENBANK/NM_174445.2
SI  - GENBANK/NM_176645.3
SI  - GENBANK/XM_010806226.1
PT  - Journal Article
DEP - 20160822
PL  - Germany
TA  - Reprod Domest Anim
JT  - Reproduction in domestic animals = Zuchthygiene
JID - 9015668
RN  - 0 (RNA, Messenger)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 3.6.5.2 (Monomeric GTP-Binding Proteins)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Female
MH  - Gene Expression Regulation/*physiology
MH  - Granulosa Cells/*physiology
MH  - Luteinizing Hormone/*physiology
MH  - Monomeric GTP-Binding Proteins/genetics/metabolism
MH  - Ovulation/*physiology
MH  - RNA, Messenger/genetics/metabolism
MH  - Signal Transduction
MH  - Sirolimus/administration & dosage/pharmacology
MH  - TOR Serine-Threonine Kinases/genetics/*metabolism
EDAT- 2016/08/23 06:00
MHDA- 2017/04/11 06:00
CRDT- 2016/08/23 06:00
PHST- 2016/03/18 00:00 [received]
PHST- 2016/07/01 00:00 [accepted]
PHST- 2016/08/23 06:00 [entrez]
PHST- 2016/08/23 06:00 [pubmed]
PHST- 2017/04/11 06:00 [medline]
AID - 10.1111/rda.12745 [doi]
PST - ppublish
SO  - Reprod Domest Anim. 2016 Oct;51(5):766-73. doi: 10.1111/rda.12745. Epub 2016 Aug 
      22.