PMID- 27546822
OWN - NLM
STAT- MEDLINE
DCOM- 20170727
LR  - 20171030
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 633
DP  - 2016 Oct 28
TI  - Melatonin as a potential counter-effect of hyperalgesia induced by neonatal 
      morphine exposure.
PG  - 77-81
LID - S0304-3940(16)30606-1 [pii]
LID - 10.1016/j.neulet.2016.08.027 [doi]
AB  - Morphine administration in the neonatal period can induce long-term effects in 
      pain circuitry leading to hyperalgesia induced by the opioid in adult life. This 
      study explored a new pharmacological approach for reversing this effect of 
      morphine. We focused on melatonin owing its well-known antinociceptive and 
      anti-inflammatory effects, and its ability to interact with the opioid system. We 
      used the formalin test to assess the medium and long-term effects of melatonin 
      administration on hyperalgesia induced by morphine in early life. Newborn rats 
      were divided into two groups: the control group, which received saline, and the 
      morphine group, which received morphine (5mug subcutaneously [s.c.]) in the 
      mid-scapular area, once daily for 7days, from P8 (postnatal day 8) until P14. At 
      postnatal days 30 (P30) and 60 (P60), both groups were divided in two subgroups, 
      which received melatonin or melatonin vehicle 30min before the formalin test. The 
      nociceptive responses were assessed by analyzing the total time spent biting, 
      flicking, and licking the formalin-injected hind paw; these responses were 
      recorded during the first 5min (neurogenic/acute phase) and from 15 to 30min 
      (inflammatory/tonic phase). Initially, animals in the morphine/vehicle group 
      showed increased nociceptive behavior in phase II (inflammatory) of the formalin 
      test at P30, and in the neurogenic and inflammatory phases at P60. These 
      increased nociceptive responses were fully reversed by melatonin administration 
      at either age. These findings show that melatonin administration is a potential 
      means for countering hyperalgesia induced by neonatal morphine exposure in young 
      and adult rats.
CI  - Copyright (c) 2016 Elsevier Ireland Ltd. All rights reserved.
FAU - Rozisky, Joanna Ripoll
AU  - Rozisky JR
AD  - Pain Pharmacology and Neuromodulation Laboratory, Preclinical Research, 
      Department of Pharmacology, Institute of Basic Health Sciences, Universidade 
      Federal do Rio Grande do Sul, Porto Alegre, RS, 90050-170, Brazil; Postgraduate 
      Program in Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto 
      Alegre, RS, 90035-003, Brazil; Animal Experimentation Unit, Graduate Research 
      Group, Hospital de Clinicas de Porto Alegre, RS, 90035-003, Brazil.
FAU - Scarabelot, Vanessa Leal
AU  - Scarabelot VL
AD  - Pain Pharmacology and Neuromodulation Laboratory, Preclinical Research, 
      Department of Pharmacology, Institute of Basic Health Sciences, Universidade 
      Federal do Rio Grande do Sul, Porto Alegre, RS, 90050-170, Brazil; Postgraduate 
      Program in Biological Sciences: Physiology, Institute of Health Sciences, 
      Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, 90050-170, Brazil; 
      Animal Experimentation Unit, Graduate Research Group, Hospital de Clinicas de 
      Porto Alegre, RS, 90035-003, Brazil.
FAU - Oliveira, Carla de
AU  - Oliveira C
AD  - Pain Pharmacology and Neuromodulation Laboratory, Preclinical Research, 
      Department of Pharmacology, Institute of Basic Health Sciences, Universidade 
      Federal do Rio Grande do Sul, Porto Alegre, RS, 90050-170, Brazil; Postgraduate 
      Program in Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto 
      Alegre, RS, 90035-003, Brazil; Animal Experimentation Unit, Graduate Research 
      Group, Hospital de Clinicas de Porto Alegre, RS, 90035-003, Brazil.
FAU - Macedo, Isabel Cristina de
AU  - Macedo IC
AD  - Pain Pharmacology and Neuromodulation Laboratory, Preclinical Research, 
      Department of Pharmacology, Institute of Basic Health Sciences, Universidade 
      Federal do Rio Grande do Sul, Porto Alegre, RS, 90050-170, Brazil; Postgraduate 
      Program in Biological Sciences: Physiology, Institute of Health Sciences, 
      Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, 90050-170, Brazil; 
      Animal Experimentation Unit, Graduate Research Group, Hospital de Clinicas de 
      Porto Alegre, RS, 90035-003, Brazil.
FAU - Deitos, Alicia
AU  - Deitos A
AD  - Pain Pharmacology and Neuromodulation Laboratory, Preclinical Research, 
      Department of Pharmacology, Institute of Basic Health Sciences, Universidade 
      Federal do Rio Grande do Sul, Porto Alegre, RS, 90050-170, Brazil; Postgraduate 
      Program in Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto 
      Alegre, RS, 90035-003, Brazil; Animal Experimentation Unit, Graduate Research 
      Group, Hospital de Clinicas de Porto Alegre, RS, 90035-003, Brazil.
FAU - Laste, Gabriela
AU  - Laste G
AD  - Pain Pharmacology and Neuromodulation Laboratory, Preclinical Research, 
      Department of Pharmacology, Institute of Basic Health Sciences, Universidade 
      Federal do Rio Grande do Sul, Porto Alegre, RS, 90050-170, Brazil; Postgraduate 
      Program in Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto 
      Alegre, RS, 90035-003, Brazil; Animal Experimentation Unit, Graduate Research 
      Group, Hospital de Clinicas de Porto Alegre, RS, 90035-003, Brazil.
FAU - Caumo, Wolnei
AU  - Caumo W
AD  - Pain Pharmacology and Neuromodulation Laboratory, Preclinical Research, 
      Department of Pharmacology, Institute of Basic Health Sciences, Universidade 
      Federal do Rio Grande do Sul, Porto Alegre, RS, 90050-170, Brazil; Postgraduate 
      Program in Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto 
      Alegre, RS, 90035-003, Brazil.
FAU - Torres, Iraci L S
AU  - Torres IL
AD  - Pain Pharmacology and Neuromodulation Laboratory, Preclinical Research, 
      Department of Pharmacology, Institute of Basic Health Sciences, Universidade 
      Federal do Rio Grande do Sul, Porto Alegre, RS, 90050-170, Brazil; Postgraduate 
      Program in Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto 
      Alegre, RS, 90035-003, Brazil; Postgraduate Program in Biological Sciences: 
      Physiology, Institute of Health Sciences, Universidade Federal do Rio Grande do 
      Sul, Porto Alegre, RS, 90050-170, Brazil. Electronic address: 
      iltorres@hcpa.edu.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160818
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Analgesics, Non-Narcotic)
RN  - 0 (Narcotics)
RN  - 76I7G6D29C (Morphine)
RN  - JL5DK93RCL (Melatonin)
SB  - IM
MH  - Analgesics, Non-Narcotic/*pharmacology/therapeutic use
MH  - Animals
MH  - Animals, Newborn
MH  - Hyperalgesia/chemically induced/*drug therapy
MH  - Male
MH  - Melatonin/*pharmacology/therapeutic use
MH  - Morphine/*adverse effects
MH  - Narcotics/*adverse effects
MH  - Rats, Wistar
OTO - NOTNLM
OT  - Formalin test
OT  - Hyperalgesia
OT  - Melatonin
OT  - Morphine
OT  - Neonatal rats
EDAT- 2016/10/22 06:00
MHDA- 2017/07/28 06:00
CRDT- 2016/08/23 06:00
PHST- 2016/05/20 00:00 [received]
PHST- 2016/08/11 00:00 [revised]
PHST- 2016/08/15 00:00 [accepted]
PHST- 2016/10/22 06:00 [pubmed]
PHST- 2017/07/28 06:00 [medline]
PHST- 2016/08/23 06:00 [entrez]
AID - S0304-3940(16)30606-1 [pii]
AID - 10.1016/j.neulet.2016.08.027 [doi]
PST - ppublish
SO  - Neurosci Lett. 2016 Oct 28;633:77-81. doi: 10.1016/j.neulet.2016.08.027. Epub 
      2016 Aug 18.