PMID- 27549169
OWN - NLM
STAT- MEDLINE
DCOM- 20171002
LR  - 20180123
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 197
IP  - 7
DP  - 2016 Oct 1
TI  - Dendritic Cells Are Dispensable for T Cell Priming and Control of Acute 
      Lymphocytic Choriomeningitis Virus Infection.
PG  - 2780-6
LID - 10.4049/jimmunol.1502582 [doi]
AB  - Dendritic cells (DCs) are considered to be the major APCs with potent activity 
      for priming of naive CD4 and CD8 T cells. However, T cell priming can also be 
      achieved by other APCs including macrophages, B cells, or even nonhematopoietic 
      cell types. Systemic low-dose infection of mice with lymphocytic choriomeningitis 
      virus (LCMV) results in massive expansion of virus-specific CD4 and CD8 T cells. 
      To determine the role of DCs as APCs and source of type I IFNs in this infection 
      model, we used DeltaDC mice in which DCs are constitutively ablated because of 
      expression of the diphtheria toxin alpha subunit within developing DCs. DeltaDC mice 
      showed lower serum concentrations of IFN-beta and IL-12p40, but normal IFN-alpha levels 
      during the first days postinfection. No differences were found for proliferation 
      of transferred TCR-transgenic cells during the early phase of infection, 
      suggesting that T cell priming occurred with the same efficiency in wild-type and 
      DeltaDC mice. Expansion and cytokine expression of endogenous LCMV-specific T cells 
      was comparable between wild-type and DeltaDC mice during primary infection and upon 
      rechallenge of memory mice. In both strains of infected mice the viral load was 
      reduced below the limit of detection with the same kinetic. Further, germinal 
      center formation and LCMV-specific Ab responses were not impaired in DeltaDC mice. 
      This indicates that DCs are dispensable as APCs for protective immunity against 
      LCMV infection.
CI  - Copyright (c) 2016 by The American Association of Immunologists, Inc.
FAU - Hilpert, Cornelia
AU  - Hilpert C
AUID- ORCID: 0000-0002-8761-9206
AD  - Department of Infection Biology, University Hospital Erlangen and Friedrich 
      Alexander University Erlangen-Nuremberg, 91054 Erlangen, Germany.
FAU - Sitte, Selina
AU  - Sitte S
AD  - Department of Infection Biology, University Hospital Erlangen and Friedrich 
      Alexander University Erlangen-Nuremberg, 91054 Erlangen, Germany.
FAU - Matthies, Alexander
AU  - Matthies A
AD  - Department of Infection Biology, University Hospital Erlangen and Friedrich 
      Alexander University Erlangen-Nuremberg, 91054 Erlangen, Germany.
FAU - Voehringer, David
AU  - Voehringer D
AD  - Department of Infection Biology, University Hospital Erlangen and Friedrich 
      Alexander University Erlangen-Nuremberg, 91054 Erlangen, Germany 
      david.voehringer@uk-erlangen.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160822
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Antigen Presentation
MH  - *Dendritic Cells/immunology
MH  - Lymphocyte Activation
MH  - Lymphocytic Choriomeningitis/*immunology/*virology
MH  - Lymphocytic choriomeningitis virus/*immunology
MH  - Macrophages/immunology
MH  - Mice
MH  - Mice, Congenic
MH  - Mice, Transgenic
MH  - T-Lymphocytes/*immunology
EDAT- 2016/08/24 06:00
MHDA- 2017/10/03 06:00
CRDT- 2016/08/24 06:00
PHST- 2015/12/11 00:00 [received]
PHST- 2016/07/22 00:00 [accepted]
PHST- 2016/08/24 06:00 [entrez]
PHST- 2016/08/24 06:00 [pubmed]
PHST- 2017/10/03 06:00 [medline]
AID - jimmunol.1502582 [pii]
AID - 10.4049/jimmunol.1502582 [doi]
PST - ppublish
SO  - J Immunol. 2016 Oct 1;197(7):2780-6. doi: 10.4049/jimmunol.1502582. Epub 2016 Aug 
      22.