PMID- 27550014 OWN - NLM STAT- MEDLINE DCOM- 20170613 LR - 20181113 IS - 1573-4838 (Electronic) IS - 0957-4530 (Linking) VI - 27 IP - 9 DP - 2016 Sep TI - Novel 3D scaffold with enhanced physical and cell response properties for bone tissue regeneration, fabricated by patterned electrospinning/electrospraying. PG - 143 LID - 10.1007/s10856-016-5748-8 [doi] AB - Developing three dimensional scaffolds mimicking the nanoscale structure of native extracellular matrix is a key parameter in tissue regeneration. In this study, we aimed to introduce a novel 3D structures composed of nanofibers (NF) and micro particles (MP) and compare their efficiency with 2D nanofibrous scaffold. The conventional nanofibrous PCL scaffolds are 2D mats fabricated by the electrospinning technique, whereas the NF/MP and patterned NF/MP PCL scaffolds are three dimensional structures fabricated by a modified electrospinning/electrospraying technique. The mentioned method was carried out by varying the electrospinning solution parameters and use of a metal mesh as the collector. Detailed fabrication process and morphological properties of the fabricated structures is discussed and porosity, pore size and PBS solution absorption value of the prepared structures are reported. Compared with the 2D structure, 3D scaffolds possessed enhanced porosity and pore size which led to the significant increase in their water uptake capacity. In vitro cell experiments were carried out on the prepared structures by the use of MG-63 osteosarcoma cell line. The fabricated 3D structures offered significantly increased cell attachment, spread and diffusion which were confirmed by SEM analysis. In vitro cytocompatibility assessed by MTT colorimetric assay indicated a continuous cell proliferation over 21 days on the innovative 3D structure, while on 2D mat cell proliferation stopped at early time points. Enhanced osteogenic differentiation of the seeded MG-63 cells on 3D scaffold was confirmed by the remarkable ALP activity together with increased and accelerated calcium deposition on this structure compared to 2D mat. Massive and well distributed bone minerals formed on patterned 3D structure were shown by EDX analysis. In comparison between NF/MP quasi-3D and Patterned NF/MP 3D scaffolds, patterned structures proceeded in all of the above properties. As such, the innovative Patterned NF/MP 3D scaffold could be considered as a proper bone graft substitute for bone tissue regeneration. FAU - Hejazi, Fatemeh AU - Hejazi F AD - Department of Polymer Engineering and Color Technology, Amirkabir University of Technology (Tehran Polytechnic), 424 Hafez Avenue, 1591634311, Tehran, Iran. FAU - Mirzadeh, Hamid AU - Mirzadeh H AD - Department of Polymer Engineering and Color Technology, Amirkabir University of Technology (Tehran Polytechnic), 424 Hafez Avenue, 1591634311, Tehran, Iran. mirzadeh@aut.ac.ir. LA - eng PT - Journal Article DEP - 20160822 PL - United States TA - J Mater Sci Mater Med JT - Journal of materials science. Materials in medicine JID - 9013087 RN - 0 (Phosphates) RN - 0 (Polyesters) RN - 059QF0KO0R (Water) RN - 24980-41-4 (polycaprolactone) RN - EC 3.1.3.1 (Alkaline Phosphatase) RN - SY7Q814VUP (Calcium) SB - IM MH - Alkaline Phosphatase/chemistry MH - *Bone Regeneration MH - Bone and Bones/pathology MH - Calcium/chemistry MH - Cell Differentiation MH - Cell Line, Tumor MH - Cell Proliferation MH - Colorimetry MH - Electric Conductivity MH - Humans MH - Microscopy, Electron, Scanning MH - Microspheres MH - Nanofibers MH - Osteoblasts/cytology MH - *Osteogenesis MH - Phosphates/chemistry MH - Polyesters/chemistry MH - Porosity MH - Regeneration MH - Tissue Engineering/*methods MH - Tissue Scaffolds/*chemistry MH - Viscosity MH - Water/chemistry EDAT- 2016/08/24 06:00 MHDA- 2017/06/14 06:00 CRDT- 2016/08/24 06:00 PHST- 2016/04/13 00:00 [received] PHST- 2016/07/05 00:00 [accepted] PHST- 2016/08/24 06:00 [entrez] PHST- 2016/08/24 06:00 [pubmed] PHST- 2017/06/14 06:00 [medline] AID - 10.1007/s10856-016-5748-8 [pii] AID - 10.1007/s10856-016-5748-8 [doi] PST - ppublish SO - J Mater Sci Mater Med. 2016 Sep;27(9):143. doi: 10.1007/s10856-016-5748-8. Epub 2016 Aug 22.