PMID- 27551314
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160823
LR  - 20200930
IS  - 1555-8932 (Print)
IS  - 1865-3499 (Electronic)
IS  - 1555-8932 (Linking)
VI  - 11
DP  - 2016
TI  - Differences in genome-wide gene expression response in peripheral blood 
      mononuclear cells between young and old men upon caloric restriction.
PG  - 13
LID - 10.1186/s12263-016-0528-0 [doi]
LID - 13
AB  - BACKGROUND: Caloric restriction (CR) is considered to increase lifespan and to 
      prevent various age-related diseases in different nonhuman organisms. Only a 
      limited number of CR studies have been performed on humans, and results put CR as 
      a beneficial tool to decrease risk factors in several age-related diseases. The 
      question remains at what age CR should be implemented to be most effective with 
      respect to healthy aging. The aim of our study was to elucidate the role of age 
      in the transcriptional response to a completely controlled 30 % CR diet on immune 
      cells, as immune response is affected during aging. Ten healthy young men, aged 
      20-28, and nine healthy old men, aged 64-85, were subjected to a 2-week weight 
      maintenance diet, followed by 3 weeks of 30 % CR. Before and after 30 % CR, the 
      whole genome gene expression in peripheral blood mononuclear cells (PBMCs) was 
      assessed. RESULTS: Expression of 554 genes showed a different response between 
      young and old men upon CR. Gene set enrichment analysis revealed a downregulation 
      of gene sets involved in the immune response in young but not in old men. At 
      baseline, immune response-related genes were higher expressed in old compared to 
      young men. Upstream regulator analyses revealed that most potential regulators 
      were controlling the immune response. CONCLUSIONS: Based on the gene expression 
      data, we theorise that a short period of CR is not effective in old men regarding 
      immune-related pathways while it is effective in young men. TRIAL REGISTRATION: 
      ClinicalTrials.gov, NCT00561145.
FAU - Van Bussel, I P G
AU  - Van Bussel IP
AD  - Division of Human Nutrition, Wageningen University, Bomenweg 2, 6703 HD 
      Wageningen, The Netherlands.
FAU - Jolink-Stoppelenburg, A
AU  - Jolink-Stoppelenburg A
AD  - Division of Human Nutrition, Wageningen University, Bomenweg 2, 6703 HD 
      Wageningen, The Netherlands.
FAU - De Groot, C P G M
AU  - De Groot CP
AD  - Division of Human Nutrition, Wageningen University, Bomenweg 2, 6703 HD 
      Wageningen, The Netherlands.
FAU - Muller, M R
AU  - Muller MR
AD  - Division of Human Nutrition, Wageningen University, Bomenweg 2, 6703 HD 
      Wageningen, The Netherlands ; Current Address: Norwich Medical School, University 
      of East Anglia, Norwich, NR4 7TJ UK.
FAU - Afman, L A
AU  - Afman LA
AD  - Division of Human Nutrition, Wageningen University, Bomenweg 2, 6703 HD 
      Wageningen, The Netherlands ; Division of Human Nutrition, Wageningen University 
      & Research centre, PO BOX 8129, NL-6700 EV Wageningen, The Netherlands.
LA  - eng
SI  - ClinicalTrials.gov/NCT00561145
PT  - Journal Article
DEP - 20160506
PL  - Germany
TA  - Genes Nutr
JT  - Genes & nutrition
JID - 101280108
PMC - PMC4968441
OTO - NOTNLM
OT  - Age
OT  - Caloric restriction
OT  - Gene expression
OT  - Microarray
OT  - Peripheral blood mononuclear cells
EDAT- 2016/08/24 06:00
MHDA- 2016/08/24 06:01
PMCR- 2016/05/06
CRDT- 2016/08/24 06:00
PHST- 2015/12/08 00:00 [received]
PHST- 2016/04/19 00:00 [accepted]
PHST- 2016/08/24 06:00 [entrez]
PHST- 2016/08/24 06:00 [pubmed]
PHST- 2016/08/24 06:01 [medline]
PHST- 2016/05/06 00:00 [pmc-release]
AID - 528 [pii]
AID - 10.1186/s12263-016-0528-0 [doi]
PST - epublish
SO  - Genes Nutr. 2016 May 6;11:13. doi: 10.1186/s12263-016-0528-0. eCollection 2016.