PMID- 27553280
OWN - NLM
STAT- MEDLINE
DCOM- 20170529
LR  - 20211204
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 478
IP  - 3
DP  - 2016 Sep 23
TI  - The collagen derived dipeptide hydroxyprolyl-glycine promotes C2C12 myoblast 
      differentiation and myotube hypertrophy.
PG  - 1292-7
LID - S0006-291X(16)31378-X [pii]
LID - 10.1016/j.bbrc.2016.08.114 [doi]
AB  - The majority of studies on possible roles for collagen hydrolysates in human 
      health have focused on their effects on bone and skin. Hydroxyprolyl-glycine 
      (Hyp-Gly) was recently identified as a novel collagen hydrolysate-derived 
      dipeptide in human blood. However, any possible health benefits of Hyp-Gly remain 
      unclear. Here, we report the effects of Hyp-Gly on differentiation and 
      hypertrophy of murine skeletal muscle C2C12 cells. Hyp-Gly increased the fusion 
      index, the myotube size, and the expression of the myotube-specific myosin heavy 
      chain (MyHC) and tropomyosin structural proteins. Hyp-Gly increased the 
      phosphorylation of Akt, mTOR, and p70S6K in myoblasts, whereas the 
      phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 inhibited their 
      phosphorylation by Hyp-Gly. LY294002 and the mammalian target of rapamycin (mTOR) 
      inhibitor rapamycin repressed the enhancing effects of Hyp-Gly on MyHC and 
      tropomyosin expression. The peptide/histidine transporter 1 (PHT1) was highly 
      expressed in both myoblasts and myotubes, and co-administration of histidine 
      inhibited Hyp-Gly-induced phosphorylation of p70S6K in myoblasts and myotubes. 
      These results indicate that Hyp-Gly can induce myogenic differentiation and 
      myotube hypertrophy and suggest that Hyp-Gly promotes myogenic differentiation by 
      activating the PI3K/Akt/mTOR signaling pathway, perhaps depending on PHT1 for 
      entry into cells.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Kitakaze, Tomoya
AU  - Kitakaze T
AD  - Division of Applied Life Sciences, Graduate School of Life and Environmental 
      Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 
      5998531, Japan.
FAU - Sakamoto, Tomotaka
AU  - Sakamoto T
AD  - Division of Applied Life Sciences, Graduate School of Life and Environmental 
      Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 
      5998531, Japan.
FAU - Kitano, Takehiro
AU  - Kitano T
AD  - Division of Applied Life Sciences, Graduate School of Life and Environmental 
      Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 
      5998531, Japan.
FAU - Inoue, Naoki
AU  - Inoue N
AD  - Nitta Gelatin Inc., Peptide Division, 2-22 Futamata, Yao, Osaka, 5810024, Japan.
FAU - Sugihara, Fumihito
AU  - Sugihara F
AD  - Nitta Gelatin Inc., Peptide Division, 2-22 Futamata, Yao, Osaka, 5810024, Japan.
FAU - Harada, Naoki
AU  - Harada N
AD  - Division of Applied Life Sciences, Graduate School of Life and Environmental 
      Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 
      5998531, Japan.
FAU - Yamaji, Ryoichi
AU  - Yamaji R
AD  - Division of Applied Life Sciences, Graduate School of Life and Environmental 
      Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 
      5998531, Japan. Electronic address: yamaji@biochem.osakafu-u.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20160821
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Dipeptides)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Slc15a4 protein, mouse)
RN  - 0 (Tropomyosin)
RN  - 0 (hydroxyprolyl-glycine)
RN  - 4QD397987E (Histidine)
RN  - 9007-34-5 (Collagen)
RN  - 97599-47-8 (peptide permease)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 3.6.4.1 (Myosin Heavy Chains)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*drug effects
MH  - Cell Size/drug effects
MH  - Collagen/*pharmacology
MH  - Dipeptides/*pharmacology
MH  - Histidine/pharmacology
MH  - Hypertrophy
MH  - Male
MH  - Membrane Transport Proteins/metabolism
MH  - Mice
MH  - Muscle Fibers, Skeletal/drug effects/metabolism/*pathology
MH  - Myoblasts/*cytology/drug effects/metabolism
MH  - Myosin Heavy Chains/metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Ribosomal Protein S6 Kinases, 70-kDa/metabolism
MH  - Signal Transduction/drug effects
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Tropomyosin/metabolism
OTO - NOTNLM
OT  - Collagen peptide
OT  - Differentiation
OT  - Hydroxyprolyl-glycine
OT  - Hypertrophy
OT  - Myoblast
OT  - Myotube
EDAT- 2016/08/25 06:00
MHDA- 2017/05/30 06:00
CRDT- 2016/08/25 06:00
PHST- 2016/08/05 00:00 [received]
PHST- 2016/08/19 00:00 [accepted]
PHST- 2016/08/25 06:00 [entrez]
PHST- 2016/08/25 06:00 [pubmed]
PHST- 2017/05/30 06:00 [medline]
AID - S0006-291X(16)31378-X [pii]
AID - 10.1016/j.bbrc.2016.08.114 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2016 Sep 23;478(3):1292-7. doi: 
      10.1016/j.bbrc.2016.08.114. Epub 2016 Aug 21.