PMID- 27553478
OWN - NLM
STAT- MEDLINE
DCOM- 20170804
LR  - 20181113
IS  - 1573-6849 (Electronic)
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 24
IP  - 4
DP  - 2016 Dec
TI  - Centromere inactivation on a neo-Y fusion chromosome in threespine stickleback 
      fish.
PG  - 437-450
AB  - Having one and only one centromere per chromosome is essential for proper 
      chromosome segregation during both mitosis and meiosis. Chromosomes containing 
      two centromeres are known as dicentric and often mis-segregate during cell 
      division, resulting in aneuploidy or chromosome breakage. Dicentric chromosome 
      can be stabilized by centromere inactivation, a process which reestablishes 
      monocentric chromosomes. However, little is known about this process in naturally 
      occurring dicentric chromosomes. Using a combination of fluorescence in situ 
      hybridization (FISH) and immunofluorescence combined with FISH (IF-FISH) on 
      metaphase chromosome spreads, we demonstrate that centromere inactivation has 
      evolved on a neo-Y chromosome fusion in the Japan Sea threespine stickleback fish 
      (Gasterosteus nipponicus). We found that the centromere derived from the 
      ancestral Y chromosome has been inactivated. Our data further suggest that there 
      have been genetic changes to this centromere in the two million years since the 
      formation of the neo-Y chromosome, but it remains unclear whether these genetic 
      changes are a cause or consequence of centromere inactivation.
FAU - Cech, Jennifer N
AU  - Cech JN
AD  - Divisions of Basic Sciences and Human Biology, Fred Hutchinson Cancer Research 
      Center, 1100 Fairview Ave North, Mailstop C2-023, Seattle, WA, 98109, USA.
AD  - Graduate Program in Molecular and Cellular Biology, University of Washington, 
      Seattle, WA, 98195, USA.
FAU - Peichel, Catherine L
AU  - Peichel CL
AD  - Institute of Ecology and Evolution, University of Bern, Baltzerstrasse 6, 3012, 
      Bern, Switzerland. cpeichel@fhcrc.org.
LA  - eng
GR  - P30 CA015704/CA/NCI NIH HHS/United States
GR  - R01 GM116853/GM/NIGMS NIH HHS/United States
GR  - T32 CA009657/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20160823
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
SB  - IM
MH  - Animals
MH  - Centromere/*genetics
MH  - Epigenesis, Genetic
MH  - Evolution, Molecular
MH  - In Situ Hybridization, Fluorescence
MH  - Metaphase/genetics
MH  - Smegmamorpha/*genetics
MH  - Y Chromosome/*genetics
PMC - PMC5173417
MID - NIHMS812522
OTO - NOTNLM
OT  - CENP-A
OT  - Centromere inactivation
OT  - ChIP-seq
OT  - Dicentric chromosome fusion
OT  - Gasterosteus aculeatus
OT  - Gasterosteus nipponicus
EDAT- 2016/08/25 06:00
MHDA- 2017/08/05 06:00
PMCR- 2017/12/01
CRDT- 2016/08/25 06:00
PHST- 2016/06/21 00:00 [received]
PHST- 2016/08/16 00:00 [accepted]
PHST- 2016/08/14 00:00 [revised]
PHST- 2016/08/25 06:00 [pubmed]
PHST- 2017/08/05 06:00 [medline]
PHST- 2016/08/25 06:00 [entrez]
PHST- 2017/12/01 00:00 [pmc-release]
AID - 10.1007/s10577-016-9535-7 [pii]
AID - 10.1007/s10577-016-9535-7 [doi]
PST - ppublish
SO  - Chromosome Res. 2016 Dec;24(4):437-450. doi: 10.1007/s10577-016-9535-7. Epub 2016 
      Aug 23.