PMID- 27554279
OWN - NLM
STAT- MEDLINE
DCOM- 20170327
LR  - 20181202
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 99
DP  - 2016 Oct
TI  - Rotavirus activates dendritic cells derived from umbilical cord blood monocytes.
PG  - 162-172
LID - S0882-4010(16)30132-2 [pii]
LID - 10.1016/j.micpath.2016.08.020 [doi]
AB  - Rotavirus is the most common cause of acute infectious diarrhea in human neonates 
      and infants. However, the studies aimed at dissecting the anti-virus immune 
      response have been mainly performed in adults. Dendritic cells (DCs) play a 
      crucial role in innate and acquired immune responses. Therefore, it is very 
      important to determine the response of neonatal and infant DCs to rotavirus and 
      to compare it to the response of adult DCs. Thus, we determined the response of 
      monocyte-derived DCs from umbilical cord blood (UCB) and adult peripheral blood 
      (PB) to rotavirus in vitro. It was found that the rotavirus and its genome, 
      composed of segmented doubled stranded RNA (dsRNA), induced the activation of 
      neonatal DCs, as these cells up-regulated the levels of CD40, CD86, MHC II, TLR-3 
      and TLR-4, the production of cytokines IL-6, IL-12/23p40, IL-10, TGF-beta (but not 
      of IL-12p70), and the message for TNF-alpha and IFN-beta. This activation enabled the 
      neonatal DCs to induce a strong proliferation of allogeneic CD4(+) T cells and 
      the production of IFN-gamma. Moreover, neonatal DCs could be infected by rotavirus 
      and sustain its replication. Neonatal DCs had a similar response as adult DCs 
      towards rotavirus and its genome. However, adult DCs had a biased 
      pro-inflammatory response compared to neonatal DCs, which showed a biased 
      regulatory profile, as they produced higher levels of IL-10 and TGF-beta, and were 
      less efficient in inducing a Th1 type response. So it can be concluded that 
      rotavirus and its genome can induce the activation of neonatal DCs in spite of 
      their tolerogenic bias.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Rosales-Martinez, D
AU  - Rosales-Martinez D
AD  - Laboratorio de Inmunologia Viral, Facultad de Medicina, UAEM, Cuernavaca, 
      Morelos, Mexico; Facultad de Ciencias, UNAM, Mexico, D.F, Mexico.
FAU - Gutierrez-Xicotencatl, L
AU  - Gutierrez-Xicotencatl L
AD  - CISEI-INSP, SSA, Cuernavaca, Morelos, Mexico.
FAU - Badillo-Godinez, O
AU  - Badillo-Godinez O
AD  - Laboratorio de Inmunologia Viral, Facultad de Medicina, UAEM, Cuernavaca, 
      Morelos, Mexico; Centro de Investigaciones en Dinamica Celular, UAEM, Cuernavaca, 
      Morelos, Mexico.
FAU - Lopez-Guerrero, D
AU  - Lopez-Guerrero D
AD  - Laboratorio de Inmunologia Viral, Facultad de Medicina, UAEM, Cuernavaca, 
      Morelos, Mexico.
FAU - Santana-Calderon, A
AU  - Santana-Calderon A
AD  - Centro de Investigaciones en Dinamica Celular, UAEM, Cuernavaca, Morelos, Mexico.
FAU - Cortez-Gomez, R
AU  - Cortez-Gomez R
AD  - HGR 1 del IMSS, Cuernavaca, Morelos, Mexico.
FAU - Ramirez-Pliego, O
AU  - Ramirez-Pliego O
AD  - Centro de Investigaciones en Dinamica Celular, UAEM, Cuernavaca, Morelos, Mexico.
FAU - Esquivel-Guadarrama, F
AU  - Esquivel-Guadarrama F
AD  - Laboratorio de Inmunologia Viral, Facultad de Medicina, UAEM, Cuernavaca, 
      Morelos, Mexico. Electronic address: fernando.esquivel@uaem.mx.
LA  - eng
PT  - Journal Article
DEP - 20160820
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Cytokines)
RN  - 0 (Receptors, Immunologic)
SB  - IM
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Fetal Blood
MH  - Flow Cytometry
MH  - Healthy Volunteers
MH  - Humans
MH  - Monocytes/immunology
MH  - Receptors, Immunologic/analysis
MH  - Rotavirus/*immunology
OTO - NOTNLM
OT  - Dendritic cells (DCs)
OT  - Doubled stranded (dsRNA)
OT  - Peripheral blood (PB)
OT  - Umbilical cord blood (UCB)
EDAT- 2016/08/25 06:00
MHDA- 2017/03/28 06:00
CRDT- 2016/08/25 06:00
PHST- 2016/03/12 00:00 [received]
PHST- 2016/07/05 00:00 [revised]
PHST- 2016/08/18 00:00 [accepted]
PHST- 2016/08/25 06:00 [pubmed]
PHST- 2017/03/28 06:00 [medline]
PHST- 2016/08/25 06:00 [entrez]
AID - S0882-4010(16)30132-2 [pii]
AID - 10.1016/j.micpath.2016.08.020 [doi]
PST - ppublish
SO  - Microb Pathog. 2016 Oct;99:162-172. doi: 10.1016/j.micpath.2016.08.020. Epub 2016 
      Aug 20.