PMID- 27554322
OWN - NLM
STAT- MEDLINE
DCOM- 20170911
LR  - 20230322
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 25
IP  - 21
DP  - 2016 Nov 1
TI  - Perivascular Mesenchymal Stem Cells in Sheep: Characterization and Autologous 
      Transplantation in a Model of Articular Cartilage Repair.
PG  - 1659-1669
LID - 10.1089/scd.2016.0165 [doi]
AB  - Previous research has indicated that purified perivascular stem cells (PSCs) have 
      increased chondrogenic potential compared to conventional mesenchymal stem cells 
      (MSCs) derived in culture. This study aimed to develop an autologous large animal 
      model for PSC transplantation and to specifically determine if implanted cells 
      are retained in articular cartilage defects. Immunohistochemistry and 
      fluorescence-activated cell sorting were used to ascertain the reactivity of 
      anti-human and anti-ovine antibodies, which were combined and used to identify 
      and isolate pericytes (CD34(-)CD45(-)CD146(+)) and adventitial cells 
      (CD34(+)CD45(-)CD146(-)). The purified cells demonstrated osteogenic, adipogenic, 
      and chondrogenic potential in culture. Autologous ovine PSCs (oPSCs) were 
      isolated, cultured, and efficiently transfected using a green fluorescence 
      protein (GFP) encoding lentivirus. The cells were implanted into articular 
      cartilage defects on the medial femoral condyle using hydrogel and collagen 
      membranes. Four weeks following implantation, the condyle was explanted and 
      confocal laser scanning microscopy demonstrated the presence of oPSCs in the 
      defect repaired with the hydrogel. These data suggest the testability in a large 
      animal of native MSC autologous grafting, thus avoiding possible biases 
      associated with xenotransplantation. Such a setting will be used in priority for 
      indications in orthopedics, at first to model articular cartilage repair.
FAU - Hindle, Paul
AU  - Hindle P
AD  - 1 MRC Centre for Regenerative Medicine, The University of Edinburgh , Edinburgh, 
      United Kingdom .
AD  - 2 Department of Trauma and Orthopaedic Surgery, The Royal Infirmary of Edinburgh 
      , Edinburgh, United Kingdom .
FAU - Baily, James
AU  - Baily J
AD  - 1 MRC Centre for Regenerative Medicine, The University of Edinburgh , Edinburgh, 
      United Kingdom .
FAU - Khan, Nusrat
AU  - Khan N
AD  - 1 MRC Centre for Regenerative Medicine, The University of Edinburgh , Edinburgh, 
      United Kingdom .
FAU - Biant, Leela C
AU  - Biant LC
AD  - 2 Department of Trauma and Orthopaedic Surgery, The Royal Infirmary of Edinburgh 
      , Edinburgh, United Kingdom .
FAU - Simpson, A Hamish R
AU  - Simpson AH
AD  - 2 Department of Trauma and Orthopaedic Surgery, The Royal Infirmary of Edinburgh 
      , Edinburgh, United Kingdom .
FAU - Peault, Bruno
AU  - Peault B
AD  - 1 MRC Centre for Regenerative Medicine, The University of Edinburgh , Edinburgh, 
      United Kingdom .
AD  - 3 The University of California , Los Angeles, Los Angeles, California.
LA  - eng
GR  - G1000816/MRC_/Medical Research Council/United Kingdom
GR  - MR/K015710/1/MRC_/Medical Research Council/United Kingdom
GR  - MR/K017047/1/MRC_/Medical Research Council/United Kingdom
GR  - MR/L012766/1/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
DEP - 20160823
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Antibodies)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate)
SB  - IM
MH  - Animals
MH  - Antibodies/immunology
MH  - Blood Vessels/*cytology
MH  - Cartilage, Articular/drug effects/*pathology
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Cross Reactions/immunology
MH  - Disease Models, Animal
MH  - Flow Cytometry
MH  - Green Fluorescent Proteins/metabolism
MH  - Hydrogel, Polyethylene Glycol Dimethacrylate/pharmacology
MH  - Immunohistochemistry
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology/drug effects
MH  - Pericytes/cytology/drug effects
MH  - Sheep
MH  - Transfection
MH  - Transplantation, Autologous
MH  - *Wound Healing/drug effects
OTO - NOTNLM
OT  - CD34+
OT  - autologous stem cell transplantation
OT  - green fluorescent protein
OT  - in vivo tracking
OT  - pericytes
OT  - sheep model
EDAT- 2016/08/25 06:00
MHDA- 2017/09/12 06:00
CRDT- 2016/08/25 06:00
PHST- 2016/08/25 06:00 [pubmed]
PHST- 2017/09/12 06:00 [medline]
PHST- 2016/08/25 06:00 [entrez]
AID - 10.1089/scd.2016.0165 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2016 Nov 1;25(21):1659-1669. doi: 10.1089/scd.2016.0165. Epub 
      2016 Aug 23.