PMID- 27554596
OWN - NLM
STAT- MEDLINE
DCOM- 20170307
LR  - 20211109
IS  - 1873-6351 (Electronic)
IS  - 0278-6915 (Linking)
VI  - 97
DP  - 2016 Nov
TI  - Increase in antioxidant activity by sheep/goat whey protein through nuclear 
      factor-like 2 (Nrf2) is cell type dependent.
PG  - 47-56
LID - S0278-6915(16)30288-5 [pii]
LID - 10.1016/j.fct.2016.08.022 [doi]
AB  - The aim of the present study was to investigate the molecular mechanisms through 
      which sheep/goat whey protein exerts its antioxidant activity. Thus, it was 
      examined whey protein's effects on the expression of transcription factor, 
      nuclear factor-like 2 (Nrf2) and on the expression and activity of a number of 
      antioxidant and phase II enzymes, superoxide dismutase (SOD), catalase (CAT), 
      heme oxygenase 1 (HO-1), synthase glutamyl cysteine (GCS) and 
      glutathione-s-transferase (GST), in muscle C2C12 and EA.hy926 endothelial cells. 
      C2C12 and EA.hy926 cells were treated with sheep/goat whey protein (0.78 and 
      3.12 mg/ml) and incubated for 3, 6, 12, 18 and 24 h. Whey protein increased 
      significantly the expression of Nrf2 only in EA.hy926 cells. Also, the expression 
      of SOD, HO-1, CAT and the activity of SOD, CAT and GST were increased 
      significantly in both cells types. The expression of GCS was increased 
      significantly only in C2C12 cells. Sheep/goat whey protein was shown for the 
      first time to exert its antioxidant activity through Nrf2-dependent mechanism in 
      endothelial cells and Nrf2-independent mechanism in muscle cells. Thus, Nrf2 
      could be a target for food supplements containing whey protein in order to 
      prevent oxidative stress damages and diseases related to endothelium.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Kerasioti, Efthalia
AU  - Kerasioti E
AD  - Department of Biochemistry-Biotechnology, University of Thessaly, Larissa 41221, 
      Greece. Electronic address: e-f-thalia@hotmail.com.
FAU - Stagos, Dimitrios
AU  - Stagos D
AD  - Department of Biochemistry-Biotechnology, University of Thessaly, Larissa 41221, 
      Greece. Electronic address: stagkos@uth.gr.
FAU - Tzimi, Aggeliki
AU  - Tzimi A
AD  - Department of Biochemistry-Biotechnology, University of Thessaly, Larissa 41221, 
      Greece. Electronic address: angetzimi@hotmail.com.
FAU - Kouretas, Dimitrios
AU  - Kouretas D
AD  - Department of Biochemistry-Biotechnology, University of Thessaly, Larissa 41221, 
      Greece. Electronic address: dkouret@uth.gr.
LA  - eng
PT  - Journal Article
DEP - 20160820
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Antioxidants)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Whey Proteins)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Cells, Cultured
MH  - Endothelium, Vascular/*drug effects/metabolism
MH  - Gene Expression Regulation/*drug effects
MH  - Goats
MH  - Myoblasts/*drug effects/metabolism
MH  - NF-E2-Related Factor 2/*genetics/metabolism
MH  - Oxidative Stress/*drug effects
MH  - Sheep
MH  - Whey Proteins/*metabolism
OTO - NOTNLM
OT  - Antioxidant
OT  - C2C12
OT  - EA.hy926
OT  - Nrf2
OT  - Whey protein
EDAT- 2016/10/30 06:00
MHDA- 2017/03/08 06:00
CRDT- 2016/08/25 06:00
PHST- 2016/07/20 00:00 [received]
PHST- 2016/07/30 00:00 [revised]
PHST- 2016/08/18 00:00 [accepted]
PHST- 2016/10/30 06:00 [pubmed]
PHST- 2017/03/08 06:00 [medline]
PHST- 2016/08/25 06:00 [entrez]
AID - S0278-6915(16)30288-5 [pii]
AID - 10.1016/j.fct.2016.08.022 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 2016 Nov;97:47-56. doi: 10.1016/j.fct.2016.08.022. Epub 2016 
      Aug 20.