PMID- 27554985
OWN - NLM
STAT- MEDLINE
DCOM- 20180918
LR  - 20181004
IS  - 1521-3773 (Electronic)
IS  - 1433-7851 (Linking)
VI  - 55
IP  - 39
DP  - 2016 Sep 19
TI  - Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in 
      Micelles.
PG  - 12068-72
LID - 10.1002/anie.201606609 [doi]
AB  - Dengue virus nonstructural protein 4B (NS4B) is a membrane protein consisting of 
      248 residues with a crucial role in virus replication and interference with the 
      host innate immunity. The dengue virus serotype 3 NS4B was reconstituted into 
      lyso-myristoyl phosphatidylglycerol (LMPG) micelles. Backbone resonance 
      assignment of NS4B was obtained using conventional solution NMR experiments. 
      Further studies suggested that NS4B contained eleven helices and six of them form 
      five potential transmembrane regions. This study provides atomic level 
      information for an important drug target to control flavivirus infections.
CI  - (c) 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Li, Yan
AU  - Li Y
AD  - Experimental Therapeutics Centre, Agency for Science, Technology and Research 
      (A*STAR), 31 Biopolis Way, Nanos, #03-01, Singapore, 138669, Singapore.
FAU - Wong, Ying Lei
AU  - Wong YL
AD  - Experimental Therapeutics Centre, Agency for Science, Technology and Research 
      (A*STAR), 31 Biopolis Way, Nanos, #03-01, Singapore, 138669, Singapore.
FAU - Lee, Michelle Yueqi
AU  - Lee MY
AD  - Experimental Therapeutics Centre, Agency for Science, Technology and Research 
      (A*STAR), 31 Biopolis Way, Nanos, #03-01, Singapore, 138669, Singapore.
FAU - Li, Qingxin
AU  - Li Q
AD  - Institute of Chemical & Engineering Sciences, Agency for Science, Technology, and 
      Research, 1 Pesek Road, Jurong Island, Singapore, 627833, Singapore.
FAU - Wang, Qing-Yin
AU  - Wang QY
AD  - Novartis Institute for Tropical Diseases, Singapore, Singapore.
FAU - Lescar, Julien
AU  - Lescar J
AD  - School of Biological Sciences, Nanyang Technological University, Singapore, 
      Singapore.
FAU - Shi, Pei-Yong
AU  - Shi PY
AD  - Department of Biochemistry & Molecular Biology, Department of Pharmacology & 
      Toxicology, Sealy Center for Structural Biology & Molecular Biophysics, 
      University of Texas Medical Branch, Galveston, TX, USA.
FAU - Kang, CongBao
AU  - Kang C
AUID- ORCID: 0000-0002-9886-9374
AD  - Experimental Therapeutics Centre, Agency for Science, Technology and Research 
      (A*STAR), 31 Biopolis Way, Nanos, #03-01, Singapore, 138669, Singapore. 
      cbkang@etc.a-star.edu.sg.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160824
PL  - Germany
TA  - Angew Chem Int Ed Engl
JT  - Angewandte Chemie (International ed. in English)
JID - 0370543
RN  - 0 (Membrane Proteins)
RN  - 0 (Micelles)
RN  - 0 (NS4B protein, Dengue virus)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Dengue/*virology
MH  - Dengue Virus/*chemistry
MH  - Humans
MH  - Membrane Proteins/*chemistry
MH  - Micelles
MH  - Nuclear Magnetic Resonance, Biomolecular
MH  - Protein Structure, Secondary
MH  - Viral Nonstructural Proteins/*chemistry
OTO - NOTNLM
OT  - NMR spectroscopy
OT  - NS4B
OT  - dengue virus
OT  - membrane protein
OT  - membrane topology
EDAT- 2016/08/25 06:00
MHDA- 2018/09/19 06:00
CRDT- 2016/08/25 06:00
PHST- 2016/07/07 00:00 [received]
PHST- 2016/08/25 06:00 [entrez]
PHST- 2016/08/25 06:00 [pubmed]
PHST- 2018/09/19 06:00 [medline]
AID - 10.1002/anie.201606609 [doi]
PST - ppublish
SO  - Angew Chem Int Ed Engl. 2016 Sep 19;55(39):12068-72. doi: 10.1002/anie.201606609. 
      Epub 2016 Aug 24.