PMID- 27555783
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160824
LR  - 20211109
IS  - 1178-6930 (Print)
IS  - 1178-6930 (Electronic)
IS  - 1178-6930 (Linking)
VI  - 9
DP  - 2016
TI  - miR-203 is involved in the laryngeal carcinoma pathogenesis via targeting VEGFA 
      and Cox-2.
PG  - 4629-37
LID - 10.2147/OTT.S96053 [doi]
AB  - The development of laryngeal squamous cell carcinoma (LSCC) is a multistep 
      process involving multiple factors. MicroRNAs, a group of important negative 
      regulators of gene expression, have also been confirmed to be involved in the 
      LSCC pathogenesis. In the present study, we compared the expression of nine 
      selected microRNAs in the LSCC tissues and adjacent nontumor tissues. We found 
      that the expression of miR-203 was significantly reduced in the LSCC tissues. 
      Predicted by using bioinformatics tools, we found that VEGFA and cyclooxygenase-2 
      (Cox-2) may be direct targets of miR-203. By subsequent determination through 
      dual-luciferase assay and Western blot, we confirmed that miR-203 suppresses the 
      expression of VEGFA and Cox-2 by directly targeting 3'-untranslated region. 
      Meanwhile, by analyzing the relationship between miR-203 and VEGFA in clinical 
      tissue samples, we found that a negative correlation existed in the expression of 
      miR-203 and VEGFA (P=0.0096, r=-0.33). Similarly, the expression of miR-203 and 
      Cox-2 also has a negative correlation (P=0.0019, r=-0.46). Subsequently, in vitro 
      functional study indicated that miR-203 played as a tumor suppressor by 
      repressing proliferation, migration, and invasion of Hep-2 cells. The 
      overexpression of VEGFA partially rescued the effect of overexpressed miR-203. 
      Overexpressed Cox-2 partially rescued the effect of miR-203 on Hep-2 cell 
      proliferation but not on the cell migration and invasion capacity. These findings 
      suggest that miR-203 plays as a tumor suppressor in LSCC, partially by regulating 
      VEGFA and Cox-2, and may serve as a potential target for therapeutic 
      intervention.
FAU - Xu, Lin
AU  - Xu L
AD  - Department of Otolaryngology, Second Affiliated Hospital of Zhejiang University 
      Medical College, Hangzhou.
FAU - Shen, Bin
AU  - Shen B
AD  - Department of Otolaryngology-Head & Neck Surgery, Shanghai Jiaotong University 
      Affiliated First People's Hospital, Shanghai.
FAU - Chen, Tingting
AU  - Chen T
AD  - Lishui Central Hospital, Lishui, Zhejiang Province, People's Republic of China.
FAU - Dong, Pin
AU  - Dong P
AD  - Department of Otolaryngology-Head & Neck Surgery, Shanghai Jiaotong University 
      Affiliated First People's Hospital, Shanghai.
LA  - eng
PT  - Journal Article
DEP - 20160726
PL  - New Zealand
TA  - Onco Targets Ther
JT  - OncoTargets and therapy
JID - 101514322
PMC - PMC4968859
OTO - NOTNLM
OT  - correlation analysis
OT  - laryngeal squamous cell carcinoma
OT  - non-coding RNA
EDAT- 2016/08/25 06:00
MHDA- 2016/08/25 06:01
PMCR- 2016/07/26
CRDT- 2016/08/25 06:00
PHST- 2016/08/25 06:00 [entrez]
PHST- 2016/08/25 06:00 [pubmed]
PHST- 2016/08/25 06:01 [medline]
PHST- 2016/07/26 00:00 [pmc-release]
AID - ott-9-4629 [pii]
AID - 10.2147/OTT.S96053 [doi]
PST - epublish
SO  - Onco Targets Ther. 2016 Jul 26;9:4629-37. doi: 10.2147/OTT.S96053. eCollection 
      2016.