PMID- 27555804
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160824
LR  - 20200930
IS  - 1476-9255 (Print)
IS  - 1476-9255 (Electronic)
IS  - 1476-9255 (Linking)
VI  - 13
IP  - 1
DP  - 2016
TI  - Monocyte chemoattractant protein-1 is not required for liver regeneration after 
      partial hepatectomy.
PG  - 28
LID - 10.1186/s12950-016-0136-1 [doi]
LID - 28
AB  - BACKGROUND: Liver regeneration following 70 % partial hepatectomy (PH) requires 
      the coordinated expression of soluble mediators produced by macrophages. Monocyte 
      chemoattractant protein-1 (MCP-1) is a potent stimulus of monocyte recruitment 
      and macrophage activation. The goal of this study was to determine how MCP-1 
      contributes to liver regeneration. METHODS: PH was performed on anesthetized 
      C57Bl/6 (wild type) and MCP-1 knockout mice, and macrophage-produced cytokines 
      and hepatocyte proliferation were measured. RESULTS: In wild type mice, hepatic 
      MCP-1 protein levels increased 4-6 h after PH, and elevated plasma MCP-1 levels 
      were detected 12 h after PH. Hepatocyte proliferation was comparable in MCP-1 
      knockout and wild type mice, as was the expression of macrophage-derived 
      cytokines, TNFalpha and IL-6, and levels of phosphorylated STAT3. The number of 
      CCR2(+) cells in the liver was similar in MCP-1 knockout and wild type mice, 
      which suggests that other chemokines may recruit CCR2(+) cells in the absence of 
      MCP-1. Studies with CCR2 knockout mice revealed that hepatocyte proliferation was 
      suppressed ~40 % compared to wild type mice 36 h after PH, but proliferation and 
      liver-body-weight ratios were similar at 48 h. CONCLUSION: These findings suggest 
      that MCP-1 is not required for PH-induced liver regeneration, yet the role of 
      CCR2 warrants further study.
FAU - Wyler, Stephanie L
AU  - Wyler SL
AD  - Department of Biological Sciences, Boise State University, 1910 University Drive, 
      Boise, ID 83725-1515 USA.
FAU - D'Ingillo, Shawna L
AU  - D'Ingillo SL
AD  - Department of Biological Sciences, Boise State University, 1910 University Drive, 
      Boise, ID 83725-1515 USA.
FAU - Lamb, Cheri L
AU  - Lamb CL
AD  - Biomolecular Sciences Ph.D. Program, Boise State University, Boise, ID 83725 USA.
FAU - Mitchell, Kristen A
AU  - Mitchell KA
AD  - Department of Biological Sciences, Boise State University, 1910 University Drive, 
      Boise, ID 83725-1515 USA ; Biomolecular Sciences Ph.D. Program, Boise State 
      University, Boise, ID 83725 USA.
LA  - eng
GR  - P20 GM103408/GM/NIGMS NIH HHS/United States
GR  - P20 GM109095/GM/NIGMS NIH HHS/United States
GR  - UL1 RR025014/RR/NCRR NIH HHS/United States
GR  - R15 DK088749/DK/NIDDK NIH HHS/United States
GR  - UL1 TR002319/TR/NCATS NIH HHS/United States
PT  - Journal Article
DEP - 20160822
PL  - England
TA  - J Inflamm (Lond)
JT  - Journal of inflammation (London, England)
JID - 101232234
PMC - PMC4994209
OTO - NOTNLM
OT  - CCR2
OT  - Kupffer cells
OT  - Liver regeneration
OT  - MCP-1
OT  - Macrophages
OT  - Partial hepatectomy
EDAT- 2016/08/25 06:00
MHDA- 2016/08/25 06:01
PMCR- 2016/08/22
CRDT- 2016/08/25 06:00
PHST- 2015/12/30 00:00 [received]
PHST- 2016/08/16 00:00 [accepted]
PHST- 2016/08/25 06:00 [entrez]
PHST- 2016/08/25 06:00 [pubmed]
PHST- 2016/08/25 06:01 [medline]
PHST- 2016/08/22 00:00 [pmc-release]
AID - 136 [pii]
AID - 10.1186/s12950-016-0136-1 [doi]
PST - epublish
SO  - J Inflamm (Lond). 2016 Aug 22;13(1):28. doi: 10.1186/s12950-016-0136-1. 
      eCollection 2016.