PMID- 27555966
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160824
LR  - 20200930
IS  - 2090-004X (Print)
IS  - 2090-0058 (Electronic)
IS  - 2090-004X (Linking)
VI  - 2016
DP  - 2016
TI  - The Blockade of IL6 Counterparts the Osmolar Stress-Induced Apoptosis in Human 
      Conjunctival Epithelial Cells.
PG  - 8350134
LID - 10.1155/2016/8350134 [doi]
LID - 8350134
AB  - To determine the effect of hyperosmolarity on cell survival/apoptosis of 
      conjunctival epithelial cells and evaluate the possible role of IL6, 
      Wong-Kilbourne derivative of Chang conjunctival cell line (WKD) was used in this 
      study. Confluent cells were incubated under different osmolarity (290 mOsm and 
      500 mOsm) with or without neutralizing IL6 antibody (50 ng/mL). The expression of 
      IL6 level was measured in the supernatant of each conditioned medium. Cell 
      viability/apoptosis assay was performed using Annexin V/Propidium Iodide (PI) and 
      Cell Counting Kit-8 (CCK-8). Western blot was conducted to measure the abundance 
      of apoptotic markers and IL6 related downstream signaling pathway. The 
      concentration of IL6 showed time-dependent increase in cells treated with 
      500 mOsm. Although apoptosis of WKD cell is increased in treated 500 mOsm for 
      24 h, apoptosis reduced in WKD cell treated 500 mOsm with anti-IL6 for 24 h. 
      Anti-IL6 inhibited the activation of JAK-STAT signaling pathway, which was 
      induced by hyperosmolarity. Hyperosmolar condition induced apoptosis in 
      conjunctival epithelial cells, along with increase of IL6 production. IL6 
      neutralizing antibody inhibited apoptosis and JAK-STAT signaling in hyperosmolar 
      condition. These findings suggested that IL6 may be involved in apoptotic change 
      and in hyperosmolarity.
FAU - Ju, Hee-Jung
AU  - Ju HJ
AD  - Catholic Institute for Visual Science, Seoul St. Mary's Hospital, Seoul 06591, 
      Republic of Korea.
FAU - Byun, Yong-Soo
AU  - Byun YS
AD  - Catholic Institute for Visual Science, Seoul St. Mary's Hospital, Seoul 06591, 
      Republic of Korea; Department of Ophthalmology, Seoul St. Mary's Hospital, 
      College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of 
      Korea.
FAU - Mok, Jee-Won
AU  - Mok JW
AD  - Catholic Institute for Visual Science, Seoul St. Mary's Hospital, Seoul 06591, 
      Republic of Korea.
FAU - Joo, Choun-Ki
AU  - Joo CK
AUID- ORCID: 0000-0003-0535-4118
AD  - Catholic Institute for Visual Science, Seoul St. Mary's Hospital, Seoul 06591, 
      Republic of Korea; Department of Ophthalmology, Seoul St. Mary's Hospital, 
      College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of 
      Korea.
LA  - eng
PT  - Journal Article
DEP - 20160731
PL  - United States
TA  - J Ophthalmol
JT  - Journal of ophthalmology
JID - 101524199
PMC - PMC4983368
EDAT- 2016/08/25 06:00
MHDA- 2016/08/25 06:01
PMCR- 2016/07/31
CRDT- 2016/08/25 06:00
PHST- 2016/01/12 00:00 [received]
PHST- 2016/05/04 00:00 [accepted]
PHST- 2016/08/25 06:00 [entrez]
PHST- 2016/08/25 06:00 [pubmed]
PHST- 2016/08/25 06:01 [medline]
PHST- 2016/07/31 00:00 [pmc-release]
AID - 10.1155/2016/8350134 [doi]
PST - ppublish
SO  - J Ophthalmol. 2016;2016:8350134. doi: 10.1155/2016/8350134. Epub 2016 Jul 31.