PMID- 27556678
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20161230
IS  - 1807-3107 (Electronic)
IS  - 1806-8324 (Linking)
VI  - 30
IP  - 1
DP  - 2016 Aug 22
TI  - Longitudinal study on clinical and microbial analysis of periodontal status in 
      pregnancy.
PG  - e87
LID - S1806-83242016000100279 [pii]
LID - 10.1590/1807-3107BOR-2016.vol30.0087 [doi]
AB  - This study was aimed to provide a longitudinal overview of the subgingival 
      bacterial microbiome using fluorescence in situ hybridization (FISH) technique, 
      in women in the second trimester of pregnancy (between 14 and 24 weeks), and 48 h 
      and 8 weeks postpartum. Of 31 women evaluated during pregnancy, 24 returned for 
      the 48-h and 18 for their 8-week exams postpartum. Probing depth (PD), bleeding 
      on probing, clinical attachment level, and presence of calculus were recorded. 
      Subgingival plaque samples were collected, and FISH was used to identify the 
      numbers of eight periodontal pathogens. Friedman test was used to compare 
      differences between follow-up examinations, followed by a multiple comparison 
      test for a post hoc pairwise comparison. Clinically, a significantly greater 
      number of teeth with PD = 4-5 mm were found during pregnancy than on postpartum 
      examinations. Microbial analysis showed a statistically significant decrease in 
      cell count over the study period for Prevotella nigrescens. P. intermedia, 
      Campylobacter rectus, and Porphyromonas gingivalis also decrease, although not 
      significantly, and Aggregatibacter actinomycetemcomitans increased. No 
      significant changes were found for Fusobacterium nucleatum, Treponema denticola, 
      or Tannerella forsythia. Our data demonstrate a change in the subgingival 
      microbiota during pregnancy, at least for P. nigrescens.
FAU - Machado, Fernanda Campos
AU  - Machado FC
AD  - Centro Universitario Estacio Juiz de Fora, School of Dentistry, Juiz de Fora, MG, 
      Brazil.
FAU - Cesar, Dioneia Evangelista
AU  - Cesar DE
AD  - Universidade Federal de Juiz de Fora - UFJF, Biological Sciences Institute, 
      Department of Ecology, Juiz de Fora, MG, Brazil.
FAU - Apolonio, Ana Carolina Morais
AU  - Apolonio AC
AD  - Universidade Federal de Juiz de Fora - UFJF, Biological Sciences Institute, 
      Department of Parasitology, Microbiology and Immunology, Juiz de Fora, MG, 
      Brazil.
FAU - Ribeiro, Luiz Claudio
AU  - Ribeiro LC
AD  - Universidade Federal de Juiz de Fora - UFJF, Department of Statistics, Juiz de 
      Fora, MG, Brazil.
FAU - Ribeiro, Rosangela Almeida
AU  - Ribeiro RA
AD  - Universidade Federal de Juiz de Fora - UFJF, School of Dentistry, Department of 
      Social and Child Dentistry, Juiz de Fora, MG, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20160822
PL  - Brazil
TA  - Braz Oral Res
JT  - Brazilian oral research
JID - 101307187
SB  - IM
MH  - Adult
MH  - Bacterial Load
MH  - Biofilms/growth & development
MH  - Dental Plaque/microbiology
MH  - Female
MH  - Gestational Age
MH  - Gingiva/*microbiology
MH  - Gram-Negative Bacteria/*isolation & purification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Longitudinal Studies
MH  - Microbiota
MH  - Periodontal Index
MH  - Periodontitis/microbiology
MH  - Periodontium/*microbiology
MH  - Postpartum Period
MH  - Pregnancy
MH  - Pregnancy Complications, Infectious/microbiology
MH  - Reference Values
MH  - Statistics, Nonparametric
MH  - Tannerella forsythia/isolation & purification
MH  - Time Factors
MH  - Young Adult
EDAT- 2016/08/25 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/08/25 06:00
PHST- 2015/10/14 00:00 [received]
PHST- 2016/05/24 00:00 [accepted]
PHST- 2016/08/25 06:00 [entrez]
PHST- 2016/08/25 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - S1806-83242016000100279 [pii]
AID - 10.1590/1807-3107BOR-2016.vol30.0087 [doi]
PST - epublish
SO  - Braz Oral Res. 2016 Aug 22;30(1):e87. doi: 10.1590/1807-3107BOR-2016.vol30.0087.