PMID- 27556910
OWN - NLM
STAT- MEDLINE
DCOM- 20170417
LR  - 20181202
IS  - 2167-9436 (Electronic)
IS  - 2167-9436 (Linking)
VI  - 35
IP  - 4
DP  - 2016 Aug
TI  - Screening and Identification of Highly Specific MAbs for Discovering Novel 
      Biomarkers of Bone Marrow Stromal Cells.
PG  - 199-211
LID - 10.1089/mab.2016.0004 [doi]
AB  - Bone marrow stromal cells (BMSCs) are very useful model systems for a better 
      understanding of cell behavior and differential gene expression. Up to now, there 
      have not been specific markers and MAbs for BMSCs that hamper the identification 
      and isolation of BMSCs populations. In this study, chicken BMSCs were isolated 
      from 1-day-old Beijing fatty chickens by adherent culture. After biological 
      characteristics were detected, the chicken BMSCs were used to immunize BALB/c 
      mice to prepare BMSCs-specific monoclonal antibodies (MAbs) by the routine 
      hybridoma technique. These MAbs were characterized by FACS analysis, 
      immunocytochemistry, immunohistochemistry, subtype identification, and Western 
      blotting assay and were used to explore markers of chicken BMSCs. Our data showed 
      that BMSCs expressing antigens CD29, CD44, and CD105, but not expressing antigens 
      CD34, CD45, and CD11b, could be isolated from postnatal chicken bone marrow and 
      hold great potential for multiline age differentiation. Meanwhile, we obtained 
      two hybridoma cell lines secreting chicken BMSCs-specific MAbs (named CHK1 and 
      CHK2), which specifically recognized the surface antigens expressed on chicken 
      BMSCs. According to our subtype identification, heavy chains of CHK1 and CHK2 
      were typed as IgG1 and IgG2b, respectively; all the light strands were kappa 
      subtype. MAbs CHK1 and CHK2 can be used to develop the detection assay and to 
      discover novel biomarkers of chicken BMSCs.
FAU - Ma, Xingyuan
AU  - Ma X
AD  - 1 School of Biotechnology and State Key Laboratory of Bioreactor Engineering, 
      East China University of Science and Technology , Shanghai, China .
FAU - Lin, Nanjing
AU  - Lin N
AD  - 1 School of Biotechnology and State Key Laboratory of Bioreactor Engineering, 
      East China University of Science and Technology , Shanghai, China .
AD  - 2 State Key Laboratory of Oncogenes and Related Genes, Renji-Med X Clinical Stem 
      Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong 
      University, Shanghai, China .
FAU - Kang, Yanyan
AU  - Kang Y
AD  - 1 School of Biotechnology and State Key Laboratory of Bioreactor Engineering, 
      East China University of Science and Technology , Shanghai, China .
FAU - Li, Linfeng
AU  - Li L
AD  - 1 School of Biotechnology and State Key Laboratory of Bioreactor Engineering, 
      East China University of Science and Technology , Shanghai, China .
AD  - 2 State Key Laboratory of Oncogenes and Related Genes, Renji-Med X Clinical Stem 
      Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong 
      University, Shanghai, China .
FAU - Zheng, Wenyun
AU  - Zheng W
AD  - 3 School of Pharmacy and Shanghai Key Laboratory of New Drug Design, East China 
      University of Science and Technology , Shanghai, China .
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Monoclon Antib Immunodiagn Immunother
JT  - Monoclonal antibodies in immunodiagnosis and immunotherapy
JID - 101590955
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/biosynthesis/*immunology
MH  - Antibody Specificity/*immunology
MH  - Antigens, CD/biosynthesis/*immunology
MH  - Biomarkers
MH  - Cell Differentiation/immunology
MH  - Chickens/immunology
MH  - Gene Expression Regulation
MH  - Humans
MH  - Mesenchymal Stem Cells/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
EDAT- 2016/08/25 06:00
MHDA- 2017/04/18 06:00
CRDT- 2016/08/25 06:00
PHST- 2016/08/25 06:00 [entrez]
PHST- 2016/08/25 06:00 [pubmed]
PHST- 2017/04/18 06:00 [medline]
AID - 10.1089/mab.2016.0004 [doi]
PST - ppublish
SO  - Monoclon Antib Immunodiagn Immunother. 2016 Aug;35(4):199-211. doi: 
      10.1089/mab.2016.0004.