PMID- 2755701
OWN - NLM
STAT- MEDLINE
DCOM- 19890907
LR  - 20071114
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 4
IP  - 7
DP  - 1989 Jul
TI  - Studies of the retinoblastoma gene in human sarcomas.
PG  - 839-43
AB  - The retinoblastoma susceptibility gene, (RB), is a tumor suppressor gene which, 
      when deleted is associated with the development of retinoblastoma. The 
      observation that children with heritable retinoblastoma frequently develop second 
      malignancies, principally sarcomas, led to the detection of similar RB gene 
      deletions in some osteosarcomas. We studied 44 unselected sarcomas from patients 
      with no antecedent retinoblastoma to determine the prevalence and nature of RB 
      gene alterations. DNA and RNA were extracted from fresh tumors and analysed by 
      Southern and Northern blotting. Three of nine osteosarcomas and 4 of 29 
      soft-tissue sarcomas had deletions of the RB gene. Four of these were 
      full-length, and three were partial deletions of the gene. RNA from 5 of the 7 
      deleted tumors was studied, and 4 cases completely lacked the RB transcript. 
      Transcripts were found in 19 of 20 tumors with an apparently intact RB gene, and 
      in all the normal tissues studied. An additional tumor lacked the RB transcript, 
      but the gene appeared intact at the DNA level. In total, 8 of 38 sarcomas were 
      found to have alterations of the RB gene. These data indicate that the RB gene is 
      inactivated in a significant number of sarcomas unrelated to retinoblastoma, and 
      that the potential role for the gene in the pathogenesis of human malignancy may 
      not be limited to retinoblastoma.
FAU - Reissmann, P T
AU  - Reissmann PT
AD  - Department of Medicine, UCLA School of Medicine.
FAU - Simon, M A
AU  - Simon MA
FAU - Lee, W H
AU  - Lee WH
FAU - Slamon, D J
AU  - Slamon DJ
LA  - eng
GR  - CA36827/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (DNA, Neoplasm)
RN  - 0 (RNA, Neoplasm)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child
MH  - Chromosome Deletion
MH  - DNA, Neoplasm/analysis
MH  - Eye Neoplasms/*genetics
MH  - Humans
MH  - Middle Aged
MH  - RNA, Neoplasm/analysis
MH  - Retinoblastoma/*genetics
MH  - Sarcoma/*genetics
MH  - *Suppression, Genetic
EDAT- 1989/07/01 00:00
MHDA- 1989/07/01 00:01
CRDT- 1989/07/01 00:00
PHST- 1989/07/01 00:00 [pubmed]
PHST- 1989/07/01 00:01 [medline]
PHST- 1989/07/01 00:00 [entrez]
PST - ppublish
SO  - Oncogene. 1989 Jul;4(7):839-43.