PMID- 27557669
OWN - NLM
STAT- MEDLINE
DCOM- 20170607
LR  - 20231105
IS  - 1472-6750 (Electronic)
IS  - 1472-6750 (Linking)
VI  - 16
IP  - 1
DP  - 2016 Aug 24
TI  - Characterization of transcription factor response kinetics in parallel.
PG  - 62
LID - 10.1186/s12896-016-0293-6 [doi]
LID - 62
AB  - BACKGROUND: Transcription factors (TFs) are effectors of cell signaling pathways 
      that regulate gene expression. TF networks are highly interconnected; one signal 
      can lead to changes in many TF levels, and one TF level can be changed by many 
      different signals. TF regulation is central to normal cell function, with altered 
      TF function being implicated in many disease conditions. Thus, measuring TF 
      levels in parallel, and over time, is crucial for understanding the impact of 
      stimuli on regulatory networks and on diseases. RESULTS: Here, we report the 
      parallel analysis of temporal TF level changes due to multiple stimuli in 
      distinct cell types. We have analyzed short-term dynamic changes in the levels of 
      nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB), signal 
      transducer and activator of transcription 3 (Stat3), cAMP response 
      element-binding protein (CREB), glucocorticoid receptor (GR), and TATA binding 
      protein (TBP), in breast and liver cancer cells after tumor necrosis factor-alpha 
      (TNF-alpha) and palmitic acid (PA) exposure. In response to both stimuli, NF-kB and 
      CREB levels were increased, Stat3 decreased, and TBP was constant. GR levels were 
      unchanged in response to TNF-alpha stimulation and increased in response to PA 
      treatment. CONCLUSIONS: Our results show significant overlap in signaling 
      initiated by TNF-alpha and by PA, with the exception that the events leading to 
      PA-mediated cytotoxicity likely also include induction of GR signaling. These 
      results further illuminate the dynamics of TF responses to cytokine and fatty 
      acid exposure, while concomitantly demonstrating the utility of parallel TF 
      measurement approaches in the analysis of biological phenomena.
FAU - Bilgin, Betul
AU  - Bilgin B
AD  - Department of Chemical Engineering and Materials Science, Michigan State 
      University, 428 S. Shaw Lane, Room 3249, Engineering Building, East Lansing, MI, 
      48824-1226, USA.
FAU - Nath, Aritro
AU  - Nath A
AD  - Genetics Program, Michigan State University, East Lansing, MI, 48824, USA.
FAU - Chan, Christina
AU  - Chan C
AD  - Department of Chemical Engineering and Materials Science, Michigan State 
      University, 428 S. Shaw Lane, Room 3249, Engineering Building, East Lansing, MI, 
      48824-1226, USA.
AD  - Department of Biochemistry and Molecular Biology, Michigan State University, East 
      Lansing, MI, 48824, USA.
FAU - Walton, S Patrick
AU  - Walton SP
AD  - Department of Chemical Engineering and Materials Science, Michigan State 
      University, 428 S. Shaw Lane, Room 3249, Engineering Building, East Lansing, MI, 
      48824-1226, USA. spwalton@egr.msu.edu.
LA  - eng
GR  - R21 RR024439/RR/NCRR NIH HHS/United States
GR  - R01 GM089866/GM/NIGMS NIH HHS/United States
GR  - R01 GM079688/GM/NIGMS NIH HHS/United States
GR  - R56 DK081768/DK/NIDDK NIH HHS/United States
GR  - R56 DK088251/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20160824
PL  - England
TA  - BMC Biotechnol
JT  - BMC biotechnology
JID - 101088663
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Gene Expression Profiling/*methods
MH  - Hep G2 Cells
MH  - Humans
MH  - Kinetics
MH  - Metabolic Clearance Rate
MH  - Neoplasm Proteins/*metabolism
MH  - Neoplasms, Experimental/*metabolism
MH  - Signal Transduction
MH  - Transcription Factors/*metabolism
MH  - Transcriptome
PMC - PMC4997724
OTO - NOTNLM
OT  - HepG2 cells
OT  - Kinetics
OT  - MDA-MB-231 cells
OT  - Palmitic acid treatment
OT  - Parallel
OT  - Transcription factors
EDAT- 2016/08/26 06:00
MHDA- 2017/06/08 06:00
PMCR- 2016/08/24
CRDT- 2016/08/26 06:00
PHST- 2016/02/25 00:00 [received]
PHST- 2016/08/16 00:00 [accepted]
PHST- 2016/08/26 06:00 [entrez]
PHST- 2016/08/26 06:00 [pubmed]
PHST- 2017/06/08 06:00 [medline]
PHST- 2016/08/24 00:00 [pmc-release]
AID - 10.1186/s12896-016-0293-6 [pii]
AID - 293 [pii]
AID - 10.1186/s12896-016-0293-6 [doi]
PST - epublish
SO  - BMC Biotechnol. 2016 Aug 24;16(1):62. doi: 10.1186/s12896-016-0293-6.