PMID- 27557695
OWN - NLM
STAT- MEDLINE
DCOM- 20171229
LR  - 20180404
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1469
DP  - 2016
TI  - Detection of Transgenes on DNA Fibers.
PG  - 181-8
LID - 10.1007/978-1-4939-4931-1_14 [doi]
AB  - Fluorescence in situ hybridization (FISH) was developed for detecting specific 
      DNA sequences directly on mitotic or meiotic chromosomes. However, the resolution 
      of FISH on chromosomes is limited by condensed structure of chromatin, and it is 
      difficult to differentiate two target sites close to each other. To overcome this 
      issue, the objects was changed to stretched DNA fibers, and this fiber FISH 
      technique has now been used for revealing genome structure at molecular level. 
      Hybridization and detection procedures of fiber FISH are common with FISH on 
      chromosomes. Therefore, application of fiber FISH is not difficult for the 
      researchers of some experience in ordinary FISH. DNA fibers can be released from 
      nuclei fixed on glass slides using a detergent. The DNA fibers were shred in FISH 
      procedure, and the resultant fragments became small bead-like shape. This makes 
      FISH signals on DNA fibers a series of dots. The size of DNA in the dot is 
      estimated to be approximately 1 kb, it corresponding to the resolution of fiber 
      FISH. This makes it possible to analyze structures of transgenes on DNA fibers in 
      detail.
FAU - Shibata, Fukashi
AU  - Shibata F
AD  - Faculty of Education, Ehime University, Matsuyama, 790-8577, Japan. 
      shibatafukashi@hotmail.com.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Antibodies)
RN  - 0 (Immunoconjugates)
RN  - 6SO6U10H04 (Biotin)
RN  - 9007-49-2 (DNA)
RN  - 9013-20-1 (Streptavidin)
SB  - IM
MH  - Antibodies/chemistry/immunology
MH  - Arabidopsis/*genetics
MH  - Biotin/chemistry
MH  - DNA/*chemistry
MH  - Immunoconjugates/chemistry/immunology
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Plants, Genetically Modified
MH  - Streptavidin/immunology
MH  - *Transgenes
OTO - NOTNLM
OT  - DNA fiber
OT  - FISH
OT  - T-DNA
OT  - Transgene
EDAT- 2016/08/26 06:00
MHDA- 2017/12/30 06:00
CRDT- 2016/08/26 06:00
PHST- 2016/08/26 06:00 [entrez]
PHST- 2016/08/26 06:00 [pubmed]
PHST- 2017/12/30 06:00 [medline]
AID - 10.1007/978-1-4939-4931-1_14 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2016;1469:181-8. doi: 10.1007/978-1-4939-4931-1_14.