PMID- 27558235 OWN - NLM STAT- MEDLINE DCOM- 20180510 LR - 20181113 IS - 1559-1182 (Electronic) IS - 0893-7648 (Linking) VI - 54 IP - 7 DP - 2017 Sep TI - Acupuncture Improves Cerebral Microenvironment in Mice with Alzheimer's Disease Treated with Hippocampal Neural Stem Cells. PG - 5120-5130 LID - 10.1007/s12035-016-0054-5 [doi] AB - Transplantation with neural stem cells (NSCs) is a promising clinical therapy for Alzheimer's disease (AD). However, the final fate of grafted NSCs is mainly determined by the host microenvironment. Therefore, this study investigated the role of Sanjiao acupuncture in the NSCs-treated hippocampus of a mouse model, senescence-accelerated mouse prone 8 (SAMP8) using Western blot, real-time fluorescent PCR, and immunofluorescence techniques. Meanwhile, we developed a co-culture model of hippocampal tissue specimens and NSCs in vitro, to observe the effects of acupuncture on survival, proliferation and differentiation of grafted NSCs using flow cytometry. Results showed that acupuncture pre- and post-NSCs transplantation significantly improved senescence-induced cognitive dysfunction (P < 0.05); upregulated the expression of basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), and brain-derived neurotrophic factor (BDNF) (P < 0.05); and also increased the count of neuron-specific nuclear protein (NeuN)- and glial fibrillary acidic protein (GFAP)-positive cells (P < 0.05). Therapeutic acupuncture may regulate the cytokine levels associated with survival, proliferation, and differentiation of NSCs in hippocampal microenvironment, to promote the repair of damaged cells, resulting in improved cognitive performance in mice. FAU - Zhao, Lan AU - Zhao L AD - First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China. lanzhao69@163.com. AD - Tianjin Key Laboratory of Acupuncture and Moxibustion, Tianjin, 300193, China. lanzhao69@163.com. FAU - Zhou, Chunlei AU - Zhou C AD - Tianjin First Center Hospital, Tianjin, 300192, China. FAU - Li, Li AU - Li L AD - First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China. AD - Tianjin Key Laboratory of Acupuncture and Moxibustion, Tianjin, 300193, China. FAU - Liu, Jianwei AU - Liu J AD - Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China. FAU - Shi, Huiyan AU - Shi H AD - First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China. AD - Tianjin Key Laboratory of Acupuncture and Moxibustion, Tianjin, 300193, China. FAU - Kan, Bohong AU - Kan B AD - First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China. AD - Tianjin Key Laboratory of Acupuncture and Moxibustion, Tianjin, 300193, China. FAU - Li, Zhen AU - Li Z AD - First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China. AD - Tianjin Key Laboratory of Acupuncture and Moxibustion, Tianjin, 300193, China. FAU - Li, Yunzhu AU - Li Y AD - Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China. FAU - Han, Jingxian AU - Han J AD - First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China. FAU - Yu, Jianchun AU - Yu J AD - First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China. yujianchun2000@hotmail.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160824 PL - United States TA - Mol Neurobiol JT - Molecular neurobiology JID - 8900963 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Glial Fibrillary Acidic Protein) SB - IM MH - *Acupuncture Therapy/methods MH - Alzheimer Disease/*therapy MH - Animals MH - Brain-Derived Neurotrophic Factor/metabolism/pharmacology MH - Cell Differentiation/*drug effects MH - Cell Proliferation/drug effects MH - Coculture Techniques MH - Glial Fibrillary Acidic Protein/metabolism MH - *Hippocampus/drug effects/metabolism MH - Male MH - Mice MH - Neural Stem Cells/*cytology MH - Neurons/metabolism MH - Stem Cell Transplantation/methods OTO - NOTNLM OT - Acupuncture OT - Alzheimer's disease OT - Microenvironment OT - Neural stem cells (NSCs) EDAT- 2016/08/26 06:00 MHDA- 2018/05/11 06:00 CRDT- 2016/08/26 06:00 PHST- 2016/02/23 00:00 [received] PHST- 2016/08/11 00:00 [accepted] PHST- 2016/08/26 06:00 [pubmed] PHST- 2018/05/11 06:00 [medline] PHST- 2016/08/26 06:00 [entrez] AID - 10.1007/s12035-016-0054-5 [pii] AID - 10.1007/s12035-016-0054-5 [doi] PST - ppublish SO - Mol Neurobiol. 2017 Sep;54(7):5120-5130. doi: 10.1007/s12035-016-0054-5. Epub 2016 Aug 24.