PMID- 27559314
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160825
LR  - 20210109
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 7
DP  - 2016
TI  - Airway and Parenchymal Strains during Bronchoconstriction in the Precision Cut 
      Lung Slice.
PG  - 309
LID - 10.3389/fphys.2016.00309 [doi]
LID - 309
AB  - The precision-cut lung slice (PCLS) is a powerful tool for studying airway 
      reactivity, but biomechanical measurements to date have largely focused on 
      changes in airway caliber. Here we describe an image processing tool that reveals 
      the associated spatio-temporal changes in airway and parenchymal strains. 
      Displacements of sub-regions within the PCLS are tracked in phase-contrast movies 
      acquired after addition of contractile and relaxing drugs. From displacement 
      maps, strains are determined across the entire PCLS or along user-specified 
      directions. In a representative mouse PCLS challenged with 10(-4)M methacholine, 
      as lumen area decreased, compressive circumferential strains were highest in the 
      50 mum closest to the airway lumen while expansive radial strains were highest in 
      the region 50-100 mum from the lumen. However, at any given distance from the 
      airway the strain distribution varied substantially in the vicinity of 
      neighboring small airways and blood vessels. Upon challenge with the relaxant 
      agonist chloroquine, although most strains disappeared, residual positive strains 
      remained a long time after addition of chloroquine, predominantly in the radial 
      direction. Taken together, these findings establish strain mapping as a new tool 
      to elucidate local dynamic mechanical events within the constricting airway and 
      its supporting parenchyma.
FAU - Hiorns, Jonathan E
AU  - Hiorns JE
AD  - School of Mathematical Sciences, University of Nottingham Nottingham, UK.
FAU - Bidan, Cecile M
AU  - Bidan CM
AD  - Laboratoire Interdisciplinaire de Physique, Centre National de la Recherche 
      Scientifique, Universite Grenoble AlpesGrenoble, France; Department of Molecular 
      Pharmacology, University of GroningenGroningen, Netherlands; Department of 
      Emergency Medicine, Beth Israel Deaconess Medical Center, Harvard Medical 
      SchoolBoston, MA, USA.
FAU - Jensen, Oliver E
AU  - Jensen OE
AD  - School of Mathematics, University of Manchester Manchester, UK.
FAU - Gosens, Reinoud
AU  - Gosens R
AD  - Department of Molecular Pharmacology, University of Groningen Groningen, 
      Netherlands.
FAU - Kistemaker, Loes E M
AU  - Kistemaker LE
AD  - Department of Molecular Pharmacology, University of Groningen Groningen, 
      Netherlands.
FAU - Fredberg, Jeffrey J
AU  - Fredberg JJ
AD  - Department of Environmental Health, Harvard School of Public Health Boston, MA, 
      USA.
FAU - Butler, Jim P
AU  - Butler JP
AD  - Department of Environmental Health, Harvard School of Public Health Boston, MA, 
      USA.
FAU - Krishnan, Ramaswamy
AU  - Krishnan R
AD  - Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Harvard 
      Medical School Boston, MA, USA.
FAU - Brook, Bindi S
AU  - Brook BS
AD  - School of Mathematical Sciences, University of Nottingham Nottingham, UK.
LA  - eng
GR  - G0901174/MRC_/Medical Research Council/United Kingdom
GR  - MR/M004643/1/MRC_/Medical Research Council/United Kingdom
GR  - P01 HL120839/HL/NHLBI NIH HHS/United States
GR  - R21 HL123522/HL/NHLBI NIH HHS/United States
PT  - Journal Article
DEP - 20160721
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
EIN - Front Physiol. 2017 Feb 28;8:117. PMID: 28261114
PMC - PMC4989902
OTO - NOTNLM
OT  - PCLS
OT  - airway smooth muscle
OT  - circumferential strain
OT  - contraction
OT  - displacements
OT  - radial strain
EDAT- 2016/08/26 06:00
MHDA- 2016/08/26 06:01
PMCR- 2016/07/21
CRDT- 2016/08/26 06:00
PHST- 2016/01/05 00:00 [received]
PHST- 2016/07/07 00:00 [accepted]
PHST- 2016/08/26 06:00 [entrez]
PHST- 2016/08/26 06:00 [pubmed]
PHST- 2016/08/26 06:01 [medline]
PHST- 2016/07/21 00:00 [pmc-release]
AID - 10.3389/fphys.2016.00309 [doi]
PST - epublish
SO  - Front Physiol. 2016 Jul 21;7:309. doi: 10.3389/fphys.2016.00309. eCollection 
      2016.