PMID- 27560095
OWN - NLM
STAT- MEDLINE
DCOM- 20170905
LR  - 20190116
IS  - 1525-6014 (Electronic)
IS  - 0148-0545 (Linking)
VI  - 40
IP  - 3
DP  - 2017 Jul
TI  - Assessment of mutagenic, hematological and oxidative stress biomarkers in liver 
      of Nile tilapia, Oreochromis niloticus (Linnaeus, 1758) in response to sublethal 
      verapamil exposure.
PG  - 286-294
LID - 10.1080/01480545.2016.1219914 [doi]
AB  - The influx of pharmaceutical drugs and their metabolites have been reported to 
      cause negative impact on aquatic biota. In this study, effects of long-term 
      exposure of verapamil on mutagenic, hematological parameters and activities of 
      the oxidative enzymes of Nile tilapia, Oreochromis niloticus were investigated 
      for 60 days exposure at the concentrations of 0.29, 0.58 and 1.15 mg L(-1) in the 
      fish liver. The exposure resulted in significantly high (p < 0.05) micronuclei 
      induction of peripheral blood cells at the peak on day 30 at 1.15 mg L(-1). 
      Compared with the control, there was significant increase (p < 0.05) in white 
      blood cell counts and red blood cell distribution width (RDW), with a reduction 
      in hemoglobin (Hb), red blood cell counts (RBCs), mean corpuscular volume (MCV) 
      and mean corpuscular hemoglobin concentration (MCHC) level as the concentration 
      of the drug increased. The indices of oxidative stress biomarkers (lipid 
      peroxidation and carbonyl protein) showed elevated level, depicting a positive 
      correlation with both time and concentration. More so, the activity of 
      energy-related parameter (Na(+ )-K(+)- ATPase) in the tissue was significantly 
      inhibited (p < 0.05) at the end of 60 days exposure period. Further, the activity 
      of catalase (CAT) was inhibited while reduced glutathione (GSH) level was 
      decreased in the liver tissue. There was increase in the activities of superoxide 
      dismutase (SOD), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) 
      after 30 days at 0.29 mg L(-1). The study demonstrated that prolonged exposure to 
      verapamil at sublethal concentration can result in mutagenic effects and 
      oxidative dysfunctions in O. niloticus.
FAU - Ajima, Malachy N O
AU  - Ajima MNO
AD  - a Department of Fisheries and Aquaculture Technology , Federal University of 
      Technology , Owerri , Nigeria and.
AD  - b Aquatic Environment and Health Management Division, ICAR - Central Institute of 
      Fisheries Education , Mumbai , India.
FAU - Pandey, Pramod K
AU  - Pandey PK
AD  - b Aquatic Environment and Health Management Division, ICAR - Central Institute of 
      Fisheries Education , Mumbai , India.
FAU - Kumar, Kundan
AU  - Kumar K
AD  - b Aquatic Environment and Health Management Division, ICAR - Central Institute of 
      Fisheries Education , Mumbai , India.
FAU - Poojary, Nalini
AU  - Poojary N
AD  - b Aquatic Environment and Health Management Division, ICAR - Central Institute of 
      Fisheries Education , Mumbai , India.
LA  - eng
PT  - Journal Article
DEP - 20160825
PL  - United States
TA  - Drug Chem Toxicol
JT  - Drug and chemical toxicology
JID - 7801723
RN  - 0 (Antioxidants)
RN  - 0 (Biomarkers)
RN  - 0 (Hemoglobins)
RN  - 0 (Mutagens)
RN  - 0 (Water Pollutants, Chemical)
RN  - CJ0O37KU29 (Verapamil)
SB  - IM
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Biomarkers/analysis
MH  - Blood Cell Count
MH  - Cichlids/*blood/genetics
MH  - Dose-Response Relationship, Drug
MH  - Hemoglobins/analysis
MH  - Lipid Peroxidation/drug effects
MH  - Micronuclei, Chromosome-Defective/*chemically induced
MH  - Micronucleus Tests
MH  - Mutagens/*toxicity
MH  - Oxidative Stress/*drug effects
MH  - Protein Carbonylation/drug effects
MH  - Verapamil/*toxicity
MH  - Water Pollutants, Chemical/*toxicity
OTO - NOTNLM
OT  - Blood parameters
OT  - fish
OT  - micronuclei
OT  - oxidative stress
OT  - toxicity
OT  - verapamil
EDAT- 2016/08/26 06:00
MHDA- 2017/09/07 06:00
CRDT- 2016/08/26 06:00
PHST- 2016/08/26 06:00 [pubmed]
PHST- 2017/09/07 06:00 [medline]
PHST- 2016/08/26 06:00 [entrez]
AID - 10.1080/01480545.2016.1219914 [doi]
PST - ppublish
SO  - Drug Chem Toxicol. 2017 Jul;40(3):286-294. doi: 10.1080/01480545.2016.1219914. 
      Epub 2016 Aug 25.