PMID- 27561408
OWN - NLM
STAT- MEDLINE
DCOM- 20180516
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Aug 26
TI  - A novel SoxB2 gene is required for maturation of sperm nucleus during 
      spermiogenesis in the Chinese mitten crab, Eriocheir sinensis.
PG  - 32139
LID - 10.1038/srep32139 [doi]
LID - 32139
AB  - SRY-related HMG box (Sox) genes are characterized by the presence of a 
      DNA-binding HMG domain and involved in a diverse range of developmental 
      processes. In this study, we identified a novel Sox gene, designated as 
      EsSoxB2-1, from the Chinese mitten crab Eriocheir sinensis. The EsSoxB2-1 encodes 
      a protein of 259 amino acids, sharing the highest identity with the beetle 
      Tribolium castaneum SOX21b. Unlike insect Sox21b, however, EsSoxB2-1 is 
      intronless and exhibits a gonad-specific expression pattern at both mRNA and 
      protein level. Two core promoters in 5' flanking region were demonstrated to be 
      essential for inducing transcriptional regulatory activity. The transcription of 
      EsSoxB2-1 mRNA begins in spermatogonia stage, while the translation of EsSOXB2-1 
      protein initiates at spermiogenesis stage. Interestingly, EsSOXB2-1 protein was 
      exclusively localized in the nucleus of spermatid and spermatozoa even at the end 
      of acrosome reaction, and was bound to the uncondensed chromatin in nucleoplasm 
      of mature spermatozoa. Knockdown of EsSoxB2-1 by RNAi leads to abnormal 
      transformation of the nucleus during spermiogenesis. Together, these findings 
      demonstrated the requirement of EsSoxB2-1 for the spermatozoa nucleus maturation 
      and also suggested that EsSoxB2-1 would be delivered into fertilized eggs along 
      with chromatins as a paternal transcription factor for regulating early embryonic 
      development.
FAU - Liu, Zhi-Qiang
AU  - Liu ZQ
AD  - Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources 
      Certificated by Ministry of Education, College of Fisheries and Life Science, 
      Shanghai Ocean University, 999 Hucheng Huan Road, Shanghai, 201306, P. R. China.
FAU - Jiang, Xue-Hui
AU  - Jiang XH
AD  - Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources 
      Certificated by Ministry of Education, College of Fisheries and Life Science, 
      Shanghai Ocean University, 999 Hucheng Huan Road, Shanghai, 201306, P. R. China.
FAU - Qi, Hai-Yan
AU  - Qi HY
AD  - Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources 
      Certificated by Ministry of Education, College of Fisheries and Life Science, 
      Shanghai Ocean University, 999 Hucheng Huan Road, Shanghai, 201306, P. R. China.
FAU - Xiong, Liang-Wei
AU  - Xiong LW
AD  - Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources 
      Certificated by Ministry of Education, College of Fisheries and Life Science, 
      Shanghai Ocean University, 999 Hucheng Huan Road, Shanghai, 201306, P. R. China.
FAU - Qiu, Gao-Feng
AU  - Qiu GF
AD  - Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources 
      Certificated by Ministry of Education, College of Fisheries and Life Science, 
      Shanghai Ocean University, 999 Hucheng Huan Road, Shanghai, 201306, P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160826
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Arthropod Proteins)
RN  - 0 (SOXB2 Transcription Factors)
SB  - IM
MH  - Animals
MH  - Arthropod Proteins/genetics/*metabolism
MH  - Brachyura/genetics/*metabolism
MH  - Cell Nucleus/genetics/*metabolism
MH  - Male
MH  - SOXB2 Transcription Factors/genetics/*metabolism
MH  - Sperm Maturation/*physiology
MH  - Spermatogenesis/*physiology
MH  - Spermatozoa/cytology/*metabolism
PMC - PMC4999818
EDAT- 2016/08/27 06:00
MHDA- 2018/05/17 06:00
PMCR- 2016/08/26
CRDT- 2016/08/27 06:00
PHST- 2016/03/14 00:00 [received]
PHST- 2016/08/03 00:00 [accepted]
PHST- 2016/08/27 06:00 [entrez]
PHST- 2016/08/27 06:00 [pubmed]
PHST- 2018/05/17 06:00 [medline]
PHST- 2016/08/26 00:00 [pmc-release]
AID - srep32139 [pii]
AID - 10.1038/srep32139 [doi]
PST - epublish
SO  - Sci Rep. 2016 Aug 26;6:32139. doi: 10.1038/srep32139.