PMID- 27563878
OWN - NLM
STAT- MEDLINE
DCOM- 20170501
LR  - 20220330
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 17
IP  - 9
DP  - 2016 Aug 23
TI  - Anti-Inflammatory Mechanism of Neural Stem Cell Transplantation in Spinal Cord 
      Injury.
LID - 10.3390/ijms17091380 [doi]
LID - 1380
AB  - Neural stem cell (NSC) transplantation has been proposed to promote functional 
      recovery after spinal cord injury. However, a detailed understanding of the 
      mechanisms of how NSCs exert their therapeutic plasticity is lacking. We 
      transplanted mouse NSCs into the injured spinal cord seven days after SCI, and 
      the Basso Mouse Scale (BMS) score was performed to assess locomotor function. The 
      anti-inflammatory effects of NSC transplantation was analyzed by 
      immunofluorescence staining of neutrophil and macrophages and the detection of 
      mRNA levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), 
      interleukin-6 (IL-6) and interleukin-12 (IL-12). Furthermore, bone marrow-derived 
      macrophages (BMDMs) were co-cultured with NSCs and followed by analyzing the mRNA 
      levels of inducible nitric oxide synthase (iNOS), TNF-alpha, IL-1beta, IL-6 and IL-10 
      with quantitative real-time PCR. The production of TNF-alpha and IL-1beta by BMDMs was 
      examined using the enzyme-linked immunosorbent assay (ELISA). Transplanted NSCs 
      had significantly increased BMS scores (p < 0.05). Histological results showed 
      that the grafted NSCs migrated from the injection site toward the injured area. 
      NSCs transplantation significantly reduced the number of neutrophils and 
      iNOS+/Mac-2+ cells at the epicenter of the injured area (p < 0.05). Meanwhile, 
      mRNA levels of TNF-alpha, IL-1beta, IL-6 and IL-12 in the NSCs transplantation group 
      were significantly decreased compared to the control group. Furthermore, NSCs 
      inhibited the iNOS expression of BMDMs and the release of inflammatory factors by 
      macrophages in vitro (p < 0.05). These results suggest that NSC transplantation 
      could modulate SCI-induced inflammatory responses and enhance neurological 
      function after SCI via reducing M1 macrophage activation and infiltrating 
      neutrophils. Thus, this study provides a new insight into the mechanisms 
      responsible for the anti-inflammatory effect of NSC transplantation after SCI.
FAU - Cheng, Zhijian
AU  - Cheng Z
AD  - Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710004, China. czj.0606@stu.xjtu.edu.cn.
FAU - Zhu, Wen
AU  - Zhu W
AD  - Intensive Care Unit, The First People's Hospital of Xianyang City, Xianyang 
      710021, China. zhuwen841008@163.com.
FAU - Cao, Kai
AU  - Cao K
AD  - Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710004, China. caokai@foxmail.com.
FAU - Wu, Fei
AU  - Wu F
AD  - Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710004, China. wfhnsx@163.com.
FAU - Li, Jin
AU  - Li J
AD  - Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710004, China. jin_lee1218@163.com.
FAU - Wang, Guoyu
AU  - Wang G
AD  - Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710004, China. wgy1509@126.com.
FAU - Li, Haopen
AU  - Li H
AD  - Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710004, China. lhp-3993@163.com.
FAU - Lu, Ming
AU  - Lu M
AD  - Neurosurgery Department, The Second Affiliated Hospital of Hunan Normal 
      University, Changsha 410003, China. lumingcs163@163.com.
FAU - Ren, Yi
AU  - Ren Y
AD  - Department of Biomedical Sciences, Florida State University, College of Medicine, 
      Tallahassee, FL 32306, USA. yren055@gmail.com.
FAU - He, Xijing
AU  - He X
AD  - Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710004, China. xijing_h@vip.tom.com.
LA  - eng
PT  - Journal Article
DEP - 20160823
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Female
MH  - Immunohistochemistry
MH  - Interleukin-10/metabolism
MH  - Interleukin-12/metabolism
MH  - Interleukin-1beta/metabolism
MH  - Interleukin-6/metabolism
MH  - Macrophages/cytology/physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neural Stem Cells/cytology/*physiology
MH  - Neutrophils/cytology/physiology
MH  - Spinal Cord Injuries/immunology/*therapy
MH  - Stem Cell Transplantation/*methods
MH  - Tumor Necrosis Factor-alpha/metabolism
PMC - PMC5037660
OTO - NOTNLM
OT  - inflammatory cytokine
OT  - macrophage
OT  - neural stem cells
OT  - spinal cord injury
COIS- The authors declare no conflict of interest.
EDAT- 2016/08/27 06:00
MHDA- 2017/05/02 06:00
PMCR- 2016/09/01
CRDT- 2016/08/27 06:00
PHST- 2016/06/20 00:00 [received]
PHST- 2016/08/08 00:00 [revised]
PHST- 2016/08/11 00:00 [accepted]
PHST- 2016/08/27 06:00 [entrez]
PHST- 2016/08/27 06:00 [pubmed]
PHST- 2017/05/02 06:00 [medline]
PHST- 2016/09/01 00:00 [pmc-release]
AID - ijms17091380 [pii]
AID - ijms-17-01380 [pii]
AID - 10.3390/ijms17091380 [doi]
PST - epublish
SO  - Int J Mol Sci. 2016 Aug 23;17(9):1380. doi: 10.3390/ijms17091380.