PMID- 27565230
OWN - NLM
STAT- MEDLINE
DCOM- 20171213
LR  - 20180507
IS  - 1879-0054 (Electronic)
IS  - 0739-7240 (Linking)
VI  - 57
DP  - 2016 Oct
TI  - Downregulation of LH and FSH receptors after hCG and eCG treatments in the 
      porcine oviduct.
PG  - 48-54
LID - S0739-7240(16)30076-5 [pii]
LID - 10.1016/j.domaniend.2016.06.003 [doi]
AB  - The influence of induction of ovulation and superovulation with eCG and hCG on LH 
      and FSH receptor levels in porcine oviducts on day 3 postcoitum was studied. In 
      experiment I, gilts were assigned into cyclic (control; n = 5) and inseminated (n 
      = 5) groups. In experiment II, there were 3 groups of animals: inseminated (n = 
      5), induced ovulation/inseminated (750 IU eCG, 500 IU hCG; n = 5) and 
      superovulated/inseminated (1500 IU eCG, 1000 IU hCG; n = 5) gilts. Oviduct 
      tissues were collected 3 d after insemination or PBS infusion. The messenger RNA 
      (mRNA) expression of FSH receptor (FSHR) and luteinizing hormone/chorionic 
      gonadotropin receptor (LH/CGR) was measured by real-time reverse transcription 
      PCR and protein levels using Western blots. Localization of LH/CGR and 
      FSHR-positive cells was studied by immunohistochemical staining. Insemination by 
      itself did not influence mRNA and protein levels of LH/CGR. However, FSHR mRNA 
      expression in the isthmus and ampulla of the oviduct was affected by insemination 
      (P < 0.05). Similarly, insemination decreased FSHR protein level in the isthmus 
      (P < 0.05). Stimulation with hCG and eCG did not affect LH/CGR and FSHR mRNA 
      expression, either in the isthmus or in the ampulla. Nevertheless, superovulation 
      decreased LH/CGR protein level in the oviductal ampulla (P < 0.05) in comparison 
      with inseminated gilts. Similarly, protein levels of FSHR in the oviductal 
      ampulla decreased after superovulation (P < 0.05). LH/CGR-positive cells were 
      observed in the mucosa as well as in smooth muscle cells of both parts of the 
      oviduct. Follicle-stimulating hormone receptor-positive cells were observed in 
      smooth muscle cells and blood vessels of the isthmus. In the ampulla, 
      FSHR-positive cells were observed in the smooth muscle as well as in the mucosa. 
      Summarizing, the present study revealed for the first time that stimulation with 
      eCG and hCG, especially in high doses, can change LH/CGR and FSHR levels in 
      porcine oviducts. This may in turn alter many signaling pathways, eg, PGs or 
      vascular endothelial growth factor synthesis, and consequently disturb the 
      oviductal environment, with possible detrimental effects on fertilization and/or 
      embryonic development.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Malysz-Cymborska, I
AU  - Malysz-Cymborska I
AD  - Department of Hormonal Action Mechanisms, Institute of Animal Reproduction and 
      Food Research of the Polish Academy of Sciences, Olsztyn, Poland.
FAU - Andronowska, A
AU  - Andronowska A
AD  - Department of Hormonal Action Mechanisms, Institute of Animal Reproduction and 
      Food Research of the Polish Academy of Sciences, Olsztyn, Poland. Electronic 
      address: a.andronowska@pan.olsztyn.pl.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20160616
PL  - United States
TA  - Domest Anim Endocrinol
JT  - Domestic animal endocrinology
JID - 8505191
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, FSH)
RN  - 0 (Receptors, LH)
SB  - IM
MH  - Animals
MH  - Chorionic Gonadotropin/*pharmacology
MH  - Down-Regulation
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Horses
MH  - Humans
MH  - Oviducts/*drug effects/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, FSH/genetics/*metabolism
MH  - Receptors, LH/genetics/*metabolism
MH  - Swine/*physiology
OTO - NOTNLM
OT  - FSH receptor
OT  - LHCGR
OT  - Oviduct
OT  - eCG
OT  - hCG
EDAT- 2016/08/28 06:00
MHDA- 2017/12/14 06:00
CRDT- 2016/08/28 06:00
PHST- 2015/11/14 00:00 [received]
PHST- 2016/05/30 00:00 [revised]
PHST- 2016/06/07 00:00 [accepted]
PHST- 2016/08/28 06:00 [entrez]
PHST- 2016/08/28 06:00 [pubmed]
PHST- 2017/12/14 06:00 [medline]
AID - S0739-7240(16)30076-5 [pii]
AID - 10.1016/j.domaniend.2016.06.003 [doi]
PST - ppublish
SO  - Domest Anim Endocrinol. 2016 Oct;57:48-54. doi: 10.1016/j.domaniend.2016.06.003. 
      Epub 2016 Jun 16.