PMID- 27565232
OWN - NLM
STAT- MEDLINE
DCOM- 20171213
LR  - 20211204
IS  - 1879-0054 (Electronic)
IS  - 0739-7240 (Linking)
VI  - 57
DP  - 2016 Oct
TI  - Different dietary energy intake affects skeletal muscle development through an 
      Akt-dependent pathway in Dorper x Small Thin-Tailed crossbred ewe lambs.
PG  - 63-70
LID - S0739-7240(16)30062-5 [pii]
LID - 10.1016/j.domaniend.2016.05.010 [doi]
AB  - The objective of this experiment was to investigate the mechanisms through which 
      different levels of dietary energy affect postnatal skeletal muscle development 
      in ewe lambs. Twelve Dorper x Small Thin-Tailed crossbred ewe lambs (100 d of 
      age; 20 +/- 0.5 kg BW) were selected randomly and divided into 2 groups in a 
      completely randomized design. Animals were offered identical diets at 100% or 65% 
      of ad libitum intake. Lambs were euthanized when BW in the ad libitum group 
      reached 35 kg and the semitendinosus muscle was sampled. Final BW and skeletal 
      muscle weight were decreased (P < 0.01) by feed restriction. Both muscle fiber 
      size distribution and myofibril cross-sectional area were altered by feed 
      restriction. Insulin-like growth factor 1 (IGF-1) messenger RNA (mRNA) content 
      was decreased (P < 0.05) when lambs were underfed, whereas no difference for 
      IGF-2 mRNA expression was observed (P > 0.05). Feed restriction altered 
      phosphor-Akt protein abundance (P < 0.01). Moreover, the mammalian target of 
      rapamycin (mTOR) pathway was inhibited by feed restriction, which was associated 
      with decreased phosphor-mTOR, phosphorylated eukaryotic initiation factor 4E 
      binding protein 1 (phosphor-4EBP1), and phosphorylated ribosomal protein S6 
      kinase (phosphor-S6K). Both mRNA expression of myostatin and its protein content 
      were elevated in feed-restricted ewe lambs (P < 0.05). In addition, mRNA 
      expression of both muscle RING finger 1 and muscle atrophy F-box was increased 
      when ewe lambs were underfed. In summary, feed restriction in young growing ewe 
      lambs attenuates skeletal muscle hypertrophy by inhibiting protein synthesis and 
      increasing protein degradation, which may act through the Akt-dependent pathway.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Zhao, J X
AU  - Zhao JX
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, Shanxi 030801, P. R. China.
FAU - Liu, X D
AU  - Liu XD
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, Shanxi 030801, P. R. China.
FAU - Li, K
AU  - Li K
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, Shanxi 030801, P. R. China.
FAU - Liu, W Z
AU  - Liu WZ
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, Shanxi 030801, P. R. China.
FAU - Ren, Y S
AU  - Ren YS
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, Shanxi 030801, P. R. China.
FAU - Zhang, J X
AU  - Zhang JX
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, Shanxi 030801, P. R. China. Electronic address: 
      sxndzjx@163.com.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
DEP - 20160527
PL  - United States
TA  - Domest Anim Endocrinol
JT  - Domestic animal endocrinology
JID - 8505191
RN  - 0 (Muscle Proteins)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.3 (AMP-Activated Protein Kinase Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinase Kinases
MH  - Animal Feed/*analysis
MH  - Animal Nutritional Physiological Phenomena
MH  - Animals
MH  - Diet/*veterinary
MH  - *Energy Intake
MH  - Female
MH  - Metabolic Networks and Pathways
MH  - Muscle Proteins/genetics/metabolism
MH  - Muscle, Skeletal/*growth & development
MH  - Protein Kinases/genetics/metabolism
MH  - Proto-Oncogene Proteins c-akt/drug effects/*metabolism
MH  - Sheep/*physiology
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
OTO - NOTNLM
OT  - Akt
OT  - Feed restriction
OT  - Lamb
OT  - Skeletal muscle
OT  - mTOR
EDAT- 2016/08/28 06:00
MHDA- 2017/12/14 06:00
CRDT- 2016/08/28 06:00
PHST- 2016/02/06 00:00 [received]
PHST- 2016/05/19 00:00 [revised]
PHST- 2016/05/19 00:00 [accepted]
PHST- 2016/08/28 06:00 [entrez]
PHST- 2016/08/28 06:00 [pubmed]
PHST- 2017/12/14 06:00 [medline]
AID - S0739-7240(16)30062-5 [pii]
AID - 10.1016/j.domaniend.2016.05.010 [doi]
PST - ppublish
SO  - Domest Anim Endocrinol. 2016 Oct;57:63-70. doi: 10.1016/j.domaniend.2016.05.010. 
      Epub 2016 May 27.