PMID- 27565609
OWN - NLM
STAT- MEDLINE
DCOM- 20170821
LR  - 20171105
IS  - 1522-2683 (Electronic)
IS  - 0173-0835 (Linking)
VI  - 37
IP  - 23-24
DP  - 2016 Dec
TI  - Enhanced sensitivity and resolution for the analysis of paralytic shellfish 
      poisoning toxins in water using capillary electrophoresis with amperometric 
      detection and field-amplified sample injection.
PG  - 3109-3117
LID - 10.1002/elps.201600140 [doi]
AB  - The profiling of the most lethal paralytic shellfish poisoning toxins (PSTs) in 
      freshwater has increased the need to establish an alternative analytical method 
      with high sensitivity and resolution. In this paper, a coupling technique of 
      field-amplified sample injection (FASI) and CE with end-column amperometric 
      detection (CE-AD) was developed to improve the detection sensitivity and 
      separation of PSTs by electrokinetically injecting a water plug of analytes to 
      the capillary filled with a high-conductivity BGE. Parameters affecting FASI and 
      CE process were carefully adjusted to achieve the highest response and 
      resolution. Separation selectivity for PSTs, especially for the analogues and 
      epimers, was greatly enhanced by using 40 mM Britton-Robinson buffer (pH 9.5) as 
      BGE, which altered the EOF and mobility of the analytes that interacted with 
      polyborate ions. Satisfactory linear relationship between peak current and 
      concentration of toxins were gained over a wide range of 1.95-254 mug/L. The 
      detection limits (S/N = 3) for five PSTs ranged from 0.63 to 3.11 mug/L, which are 
      below the health alert level in drinking water. In comparison with the up-to-date 
      reporting chromatographic methods, the FASI-CE-AD method was simple, low-cost, 
      selective, and sensitive enough for direct quantification of PSTs at very low 
      levels, implying a potential for screening and monitoring of PSTs in surface 
      waters.
CI  - (c) 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Li, Minsheng
AU  - Li M
AD  - Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & 
      Fujian Province), College of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R. 
      China.
FAU - Chen, Xiaoyan
AU  - Chen X
AD  - Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & 
      Fujian Province), College of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R. 
      China.
AD  - Jinjiang Environmental Monitoring Centre, Jinjiang, Fujian, P. R. China.
FAU - Guo, Yuan
AU  - Guo Y
AD  - Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & 
      Fujian Province), College of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R. 
      China.
FAU - Zhang, Bingyu
AU  - Zhang B
AD  - Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & 
      Fujian Province), College of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R. 
      China.
FAU - Tang, Fengxiang
AU  - Tang F
AD  - Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & 
      Fujian Province), College of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R. 
      China.
FAU - Wu, Xiaoping
AU  - Wu X
AD  - Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & 
      Fujian Province), College of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R. 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161004
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Marine Toxins)
SB  - IM
MH  - Electrophoresis, Capillary/*methods
MH  - Limit of Detection
MH  - Linear Models
MH  - Marine Toxins/*analysis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - *Shellfish Poisoning
OTO - NOTNLM
OT  - Capillary electrophoresis
OT  - End-cloumn amperometric detection
OT  - Field-amplified sample injection
OT  - Freshwater
OT  - Shellfish toxins
EDAT- 2016/08/28 06:00
MHDA- 2017/08/22 06:00
CRDT- 2016/08/28 06:00
PHST- 2016/03/16 00:00 [received]
PHST- 2016/08/22 00:00 [revised]
PHST- 2016/08/22 00:00 [accepted]
PHST- 2016/08/28 06:00 [pubmed]
PHST- 2017/08/22 06:00 [medline]
PHST- 2016/08/28 06:00 [entrez]
AID - 10.1002/elps.201600140 [doi]
PST - ppublish
SO  - Electrophoresis. 2016 Dec;37(23-24):3109-3117. doi: 10.1002/elps.201600140. Epub 
      2016 Oct 4.